Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats

Teixeira, Antônio Roberto Franchi; Molan, Nilza Trindade; Kubrusly, Márcia Saldanha; Bellodi-Privato, Marta; Coelho, Ana Maria M.; Leite, Kátia R. M.; Machado, Marcel Autran; Bacchella, Telésforo; Machado, Marcel Cerqueira César

doi:10.1590/s0102-86502009000100011

Cited by 26 publications

(35 citation statements)

References 12 publications

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“…As has been demonstrated in situations of intestinal ischemia and reperfusion 12 , renal 13 , cerebral 9 and spinal cord 11 , also in the liver of rats we can see the capacity of IPo of to minimize the damage resulting of this process.…”

Section: Discussionmentioning

confidence: 58%

“…This method was used successfully in myocardial 7 , brain 9 , kidney 10 , spinal cord 11 , intestine 12 and, recently, just one study that demonstrate its efficacy in liver 13 ischemia and reperfusion, which justifies this study.…”

Section: Introductionmentioning

confidence: 83%

“…In Brazil, Teixeira et al 13 also evaluated the postconditioning effect in rat liver, demonstrating its protective capacity. However, using different methods, using 60 minutes of ischemia, 120 minutes of reperfusion and postconditioning with 5 cycles of reperfusion and ischemia with 5 seconds each one.…”

Section: Discussionmentioning

confidence: 99%

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Postconditioning effect in the hepatic ischemia and reperfusion in rats

Santos¹,

Pontes²,

Miiji

et al. 2010

Acta Cir. Bras.

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Purpose:To evaluate the effect of postconditioning protection in liver tissue of rats submitted to ischemia and reperfusion. Methods: 25 Wistar male rats were randomized in three groups: Group A (ischemia and reperfusion -I/R), with 10 rats, which was made ischemia by vascular clamp application in hepatic hilum for 30 minutes and reperfusion for 60 minutes by removal of the clamp; Group B (Postconditioning -IPo), with 10 rats, with same procedure plus postconditioning (3 cycles of reperfusion inserted by 3 cycles of ischemia, 30 seconds for each phase, between ischemia and reperfusion phases); and Group C (Sham), with 5 rats, which took place only laparotomy and manipulation of the hepatic hilum. Specimens were examined (histological evaluation) and dosage of serum AST and ALT was made. The statistical analysis was made with t Student test, with significant difference when p<0.05. Results: In the Sham group there was no histological or enzymatic changes; In group A the mean tissue lesion was 1.6 and in group B 1.1 (p=0.014). In group A the mean ALT level was 355U/l and in group B was 175.9U/l (p=0.016). The mean of AST was 828.8U/l in group A and 295.5U/l in group B (p=0.001). Conclusion: The postconditioning was able to minimize the severity of liver injury in rats submitted to ischemia and reperfusion. Key words: Ischemia. Reperfusion. Liver. Rats. RESUMO Objetivo:Avaliar o efeito do pós-condicionamento isquêmico na proteção tecidual hepática de ratos submetidos à isquemia e reperfusão. Métodos: Foram utilizados 25 ratos da linhagem Wistar provenientes do Biotério da Universidade Federal de Mato Grosso do Sul, distribuídos em três grupos: A) grupo isquemia/reperfusão (I/R) (10 ratos), em que foi realizada isquemia e reperfusão hepática pela aplicação e retirada de clampe do hilo hepático durante 30 e 60 minutos, respectivamente; B) grupo pós-condicionamento isquêmico (PoCI) (10 ratos), em que foi realizado procedimento semelhante ao anterior, exceto pela realização do pós-condicionamento isquêmico entre as fases de isquemia e reperfusão, consistindo na retirada e colocação do clampe do hilo hepático por 30 segundos cada fase, durante 3 ciclos; grupo C (Sham) (5 ratos), em que realizou-se apenas laparotomia e manipulação do hilo hepático. Foram analisados os espécimes (avaliação histológica), dosagem sorológica de AST e ALT. Resultados: No grupo Sham não se observou alterações histológicas nem enzimáticas; no grupo A a média de lesão tecidual foi 1,6 e no grupo B 1,1 (p=0,014). No grupo A a média de ALT foi 355U/l e no grupo B 175,9U/l (p=0,016). A média de AST foi de 582,8U/l no grupo A e 295,5U/l no grupo B (p=0,001). Conclusão: O pós-condicionamento isquêmico foi capaz de minimizar a intensidade das lesões hepáticas de ratos submetidos à isquemia e reperfusão. Descritores: Isquemia. Reperfusão. Fígado. Ratos.

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Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 83%

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Postconditioning effect in the hepatic ischemia and reperfusion in rats

Santos¹,

Pontes²,

Miiji

et al. 2010

Acta Cir. Bras.

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“…Previous experiments involving hepatic ischemia demonstrated that postconditioning caused a decrease in lipid peroxidation 10 . In the present study, muscular glycogen levels in Pre and Pre-post groups were significantly greater than in the control group.…”

Section: Ischemic Pre and Postconditioning In Skeletal Muscle Injury mentioning

confidence: 99%

“…In humans, this effect is described in the heart 5 , brain 6 , kidneys 7 , liver 8 and skeletal muscle 9 . Ischemic postconditioning, which consists on producing short periods of ischemia and reperfusion just before sustained reperfusion, also produced protective effects in rats 10 , rabbits 11 , pigs 12 and humans 13 .…”

Section: Introductionmentioning

confidence: 99%

Ischemic pre and postconditioning in skeletal muscle injury produced by ischemia and reperfusion in rats

Lintz¹,

Dalio²,

Joviliano³

et al. 2013

Acta Cir. Bras.

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PURPOSE:To investigate the protective effects of ischemic pre and postconditioning, as well as the association of both methods, in skeletal muscle injury produced by ischemia and reperfusion in rats. METHODS:An experimental study was designed using 40 Wistar rats divided in four groups (n=10): Control -rats submitted to ischemia for 240 minutes (min) and reperfusion for 60 min; Ischemic preconditioning (Pre) -animals submitted to three cycles of clamping and releasing the aorta for five min before being submitted to the ischemia/reperfusion procedure; Ischemic postconditioning (Post) -rats submitted to three cycles of clamping and releasing the aorta for one min after the 240-minute ischemic phase; Ischemic pre and postconditioning (Pre-post) -animals submitted to the same procedures of Pre and Post groups. Skeletal muscle injury was evaluated by measuring serum levels of aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK); and muscular levels of malondialdehyde (MDA) and glycogen. RESULTS:AST levels were significantly higher in Pre and Pre-post groups (P<.01). There were no differences in LDH and CPK levels. Muscular MDA levels were similar. Glycogen levels were significantly higher in Pre and Pre-post groups (P<.01). CONCLUSIONS:Both preconditioning and its association with postconditioning had a protective effect by avoiding glycogen depletion in skeletal muscle in rats submitted to ischemia and reperfusion. Association of pre and postconditioning did not show advantage compared to preconditioning alone. Postconditioning alone did not show protective effect.

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Liver regeneration following partial hepatectomy is improved by enhancing the HGF/Met axis and Akt and Erk pathways after low-power laser irradiation in rats

et al. 2013

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A simple, easy, and safe procedure aiming to improve liver regeneration could be of great clinical benefit in critical situations such as major hepatectomy, trauma, or hemorrhage. Low-power laser irradiation (LPLI) has come into a wide range of use in clinical practice by inducing regeneration in healthy and injured tissues. However, the effect of LPLI on the process of liver regeneration, especially those related to the molecular mechanisms, is not fully understood. Thus, the aim of the present study was to investigate the main molecular mechanisms involved in liver regeneration of partially hepatectomized rats exposed to LPLI. We used Wistar male rats, which had their remaining liver irradiated or not with LPLI (wavelength of 632.8 nm and fluence of 65 mW/cm(2)) for 15 min after a 70% hepatectomy. We subsequently investigated hepatocyte growth factor (HGF), Met, Akt, and Erk 1/2 signaling pathways through protein expression and phosphorylation analyses along with cell proliferation (proliferating cell nuclear antigen (PCNA) and Ki-67) using immunoblotting and histological studies. Our results show that LPLI can improve liver regeneration as shown by increased HGF protein expression and the phosphorylation levels of Met, Akt, and Erk 1/2 accompanied by higher levels of the PCNA and Ki-67 protein in the remnant livers. In summary, our results suggest that LPLI may play a clinical role as a simple, fast, and easy-to-perform strategy in order to enhance the liver regenerative capacity of a small liver remnant after hepatectomy.

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Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats

Cited by 26 publications

References 12 publications

Postconditioning effect in the hepatic ischemia and reperfusion in rats

Postconditioning effect in the hepatic ischemia and reperfusion in rats

Ischemic pre and postconditioning in skeletal muscle injury produced by ischemia and reperfusion in rats

Liver regeneration following partial hepatectomy is improved by enhancing the HGF/Met axis and Akt and Erk pathways after low-power laser irradiation in rats

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