Postconditioning with inhaled hydrogen promotes survival of retinal ganglion cells in a rat model of retinal ischemia/reperfusion injury

Wang, Ruobing; Wu, Jiawen; Chen, Zeli; Xia, Fangzhou; Sun, Qi; Liu, Lin

doi:10.1016/j.brainres.2015.12.015

Cited by 37 publications

(25 citation statements)

References 33 publications

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“…For examples, The down-regulatory effects of hydrogen on TNF-α, IL-1β, and IL-6 levels are hypothesized as the reason for its protective role against ultraviolet light radiation or lipopolysaccharide. Hydrogen could attenuate TNF-α-induced activation of the NF-κB pathway 36 , 50 , 57 . H 2 protects neural cells and stimulates energy metabolism by stimulating the hormonal expression of ghrelin and fibroblast growth factor 21 (FGF 21 ) respectively.…”

Section: Discussionmentioning

confidence: 99%

“…for 14 consecutive days. As previously reported 32 , 33 , 36 , the 67% H 2 /33% O 2 mixed gas (V/V) was produced using an AMS-H-3 hydrogen-oxygen nebulizer Machine (ASCLEPIUS MEDITEC, Shanghai, China), which decomposed water via electrolysis to produce H 2 and O 2 gas. A transparent closed box (20 × 18 × 15-cm, length × width × height) was used as the gas inhalation room for the mice.…”

Section: Methodsmentioning

confidence: 98%

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Molecular hydrogen increases resilience to stress in mice

Gao

Song

Wang

et al. 2017

Sci Rep

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The inability to successfully adapt to stress produces pathological changes that can lead to depression. Molecular hydrogen has anti-oxidative and anti-inflammatory activities and neuroprotective effects. However, the potential role of molecular hydrogen in stress-related disorders is still poorly understood. The present study aims to investigate the effects of hydrogen gas on resilience to stress in mice. The results showed that repeated inhalation of hydrogen-oxygen mixed gas [67%:33% (V/V)] significantly decreased both the acute and chronic stress-induced depressive- and anxiety-like behaviors of mice, assessed by tail suspension test (TST), forced swimming test (FST), novelty suppressed feeding (NSF) test, and open field test (OFT). ELISA analyses showed that inhalation of hydrogen-oxygen mixed gas blocked CMS-induced increase in the serum levels of corticosterone, adrenocorticotropic hormone, interleukin-6, and tumor necrosis factor-α in mice exposed to chronic mild stress. Finally, inhalation of hydrogen gas in adolescence significantly increased the resilience to acute stress in early adulthood, which illustrates the long-lasting effects of hydrogen on stress resilience in mice. This was likely mediated by inhibiting the hypothalamic-pituitary-adrenal axis and inflammatory responses to stress. These results warrant further exploration for developing molecular hydrogen as a novel strategy to prevent the occurrence of stress-related disorders.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 98%

Molecular hydrogen increases resilience to stress in mice

Gao

Song

Wang

et al. 2017

Sci Rep

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“…It provided an output for the H 2 -O 2 mixture comprising 33% oxygen and 66% of 6 L per minute by electrolysis of pure water, as previous reported [9][10][11][12][13]17]. The generator with anti-static precautions and a one-way fire-retarding valve delivered the gas mixture to the patient through a nasal cannula immediately to avoid gas storage in the generator or pipeline.…”

Section: Generation and Inhalation Of Gas Mixturesmentioning

confidence: 99%

Breathing Hydrogen-Oxygen Mixture Decreases Inspiratory Effort in Patients with Tracheal Stenosis

Zhou

Zhong

et al. 2018

Respiration

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Background: Hydrogen-oxygen mixture (H₂-O₂) may reduce airway resistance in patients with acute severe tracheal stenosis, yet data supporting the clinical use of H₂-O₂ are insufficient. Objectives: To evaluate the efficacy and safety of breathing H₂-O₂ in acute severe tracheal stenosis. Methods: Thirty-five consecutive patients with severe acute tracheal stenosis were recruited in this prospective self-control study. Air, H₂-O₂ and O₂ inhalation was given in 4 consecutive breathing steps: air for 15 min, H₂-O₂ (6 L per min, H₂:O₂ = 2: 1) for 15 min, oxygen (3 L per min) for 15 min, and H₂-O₂ for 120 min. The primary endpoint was inspiratory effort as assessed by diaphragm electromyography (EMGdi); the secondary endpoints were transdiaphragmatic pressure (Pdi), Borg score, vital signs, and impulse oscillometry (IOS). The concentration of H₂ in the ambient environment was obtained with 12 monitors. Adverse reactions during the inhalation were recorded. Results: The mean reduction in the EMGdi under H₂-O₂ was 10.53 ± 6.83%. The EMGdi significantly decreased during 2 H₂-O₂ inhalation steps (Steps 2 and 4) compared with air (Step 1) and O₂ (Step 3) (52.95 ± 15.00 vs. 42.46 ± 13.90 vs. 53.20 ± 14.74 vs. 42.50 ± 14.12% for Steps 1 through 4, p < 0.05). The mean reduction in the Pdi under H₂-O₂ was 4.77 ± 3.51 cmH₂O. Breathing H₂-O₂ significantly improved the Borg score and resistance parameters of IOS but not vital signs. No adverse reactions occurred. H₂ was undetectable in the environment throughout the procedure. Conclusions: Breathing H₂-O₂ may reduce the inspiratory effort in patients with acute severe tracheal stenosis and can be used for this purpose safely.

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“…The interruption of blood flow to the retina results in the progressive loss of retinal ganglion cells (RGCs)[1] and plays the fundamental role in many ocular degenerative diseases such as diabetic retinopathy and glaucoma[2]. RGCs transmit visual information from the retina to the brain in the form of action potentials[3].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury

Zhang

Xie

et al. 2016

PLoS ONE

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Background: Brain-derived neurotrophic factor (BDNF) protects retinal ganglion cells against ischemia in ocular degenerative diseases. We aimed to determine the effect of BDNF-AS on the ischemic injury of retinal ganglion cells. Methods: The levels of BDNF and BDNF-AS were measured in retinal ganglion cells subjected to oxygen and glucose deprivation. The lentiviral vectors were constructed to either overexpress or knock out BDNF-AS. The luciferase reporter gene assay was used to determine whether BDNF-AS could target its seed sequence on BDNF mRNA. The methyl thiazolyl tetrazolium assay was used to determine cell viability, and TUNEL staining was used for cell apoptosis. Results: The levels of BDNF-AS were negatively correlated with BDNF in ischemic retinal ganglion cells. BDNF-AS directly targeted its complementary sequences on BDNF mRNA. BDNF-AS regulated the expression of BDNF and its related genes in retinal ganglion cells. Down-regulation of BDNF-AS increased cell viability and decreased the number of TUNEL-positive retinal ganglion cells under oxygen and glucose deprivation conditions. Conclusion: Inhibition of BDNF-AS protected retinal ganglion cells against ischemia by increasing the levels of BDNF.

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Postconditioning with inhaled hydrogen promotes survival of retinal ganglion cells in a rat model of retinal ischemia/reperfusion injury

Cited by 37 publications

References 33 publications

Molecular hydrogen increases resilience to stress in mice

Molecular hydrogen increases resilience to stress in mice

Breathing Hydrogen-Oxygen Mixture Decreases Inspiratory Effort in Patients with Tracheal Stenosis

Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury

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