Posthypoxic Moderate Hypothermia Improves Electrophysiological Recovery in the Rat Hippocampal Slice

Charchaflieh, Jean; Torres, Hector; Cottrell, James E.; Kass, Ira S.

doi:10.1097/ana.0b013e31820592d1

Journal of Neurosurgical Anesthesiology

2011

DOI: 10.1097/ana.0b013e31820592d1

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Posthypoxic Moderate Hypothermia Improves Electrophysiological Recovery in the Rat Hippocampal Slice

Jean Charchaflieh

Hector Torres²,

James E. Cottrell³

et al.

Abstract: Moderate hypothermia (30°C) applied after hypoxia is protective against short periods (3 min) of hypoxia but not after slightly longer periods (3.5 or 4 min) of hypoxia.

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Hypothermic preconditioning but not ketamine reduces oxygen and glucose deprivation induced neuronal injury correlated with downregulation of COX-2 expression in mouse hippocampal slices

Jiang

Fang³

et al. 2018

Journal of Pharmacological Sciences

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Hypothermic preconditioning is an effective treatment for limiting ischemic injury, but the mechanism is poorly understood. This study was aimed to explore the effect of hypothermic and ketamine preconditioning on oxygen and glucose deprivation (OGD) induced neuronal injury in mouse hippocampal slices, and to investigate its possible mechanism. The population spike (PS) was recorded in the CA1 region of mouse hippocampal slices using extracellular recordings, Na/K ATPase activity in slices was determined by spectrophotometer and the expression of Cyclooxygenase-2 (COX-2) was measured by Western blot. Ten min of OGD induced a poor recovery of PS in slices after reoxygenation. Hypothermic (33 °C) preconditioning delayed the appearance of transient recovery (TR) of PS and improved the recovery amplitude of PS after reoxygenation. Hypothermic preconditioning also decreased the expression of COX-2 and increased Na/K ATPase activity in slices. Pretreatment of ketamine, a non-competitive NMDA receptor antagonist at a subanesthetic dose has no effect on OGD induced neuronal injury. Moreover, the protection of hypothermic preconditioning was not added by ketamine. The downregulation of COX-2 expression and the increase of Na/K ATPase activity may be associated with the effectiveness of hypothermic preconditioning in increasing tolerance to an OGD insult.

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Hypothermic preconditioning but not ketamine reduces oxygen and glucose deprivation induced neuronal injury correlated with downregulation of COX-2 expression in mouse hippocampal slices

Jiang

Fang³

et al. 2018

Journal of Pharmacological Sciences

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show abstract

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Posthypoxic Moderate Hypothermia Improves Electrophysiological Recovery in the Rat Hippocampal Slice

Abstract: Moderate hypothermia (30°C) applied after hypoxia is protective against short periods (3 min) of hypoxia but not after slightly longer periods (3.5 or 4 min) of hypoxia.

Cited by 1 publication

References 7 publications

Hypothermic preconditioning but not ketamine reduces oxygen and glucose deprivation induced neuronal injury correlated with downregulation of COX-2 expression in mouse hippocampal slices

Hypothermic preconditioning but not ketamine reduces oxygen and glucose deprivation induced neuronal injury correlated with downregulation of COX-2 expression in mouse hippocampal slices

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