Postmenopausal Osteoporosis Is Associated with Serum Chemerin and Irisin but Not with Apolipoprotein M Levels

Engin-Üstün, Yaprak; Çağlayan, Emel Kıyak; Göçmen, Ayşe Yeşim; Polat, M. Fevzi

doi:10.6118/jmm.2016.22.2.76

Cited by 29 publications

(24 citation statements)

References 12 publications

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“…Engin-Üstün et al (28) reported that serum irisin levels were significantly lower in women with postmenopausal osteoporosis than in postmenopausal controls. Anastasilakis et al (29) and Palermo et al (30) found that a lower level of serum irisin was associated with osteoporotic fractures in postmenopausal women, implying that irisin may suppress osteoclastogenesis because the remarkable pathologic change in postmenopausal osteoporosis is increased osteoclastogenic activity.…”

Section: Discussionmentioning

confidence: 98%

Irisin promotes proliferation but inhibits differentiation in osteoclast precursor cells

et al. 2018

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The receptor activator of NF-κB ligand-induced osteoclast differentiation has a critical role in the process of bone metabolism. Overactivation of osteoclastogenesis may result in a series of diseases. Irisin, a novel myokine, which was first reported in 2012, has been proposed to mediate the beneficial metabolic effects of exercise. Studies have demonstrated that irisin targets osteoblasts by promoting osteoblast proliferation and differentiation; however, the underlying mechanism regarding the effect of irisin on osteoclasts remains elusive. Using 2 types of osteoclast precursor cells, RAW264.7 cells and mouse bone marrow monocytes, we showed that irisin promoted osteoclast precursor cell proliferation but inhibited osteoclast differentiation. Irisin down-regulated the expression of osteoclast differentiation marker genes, including receptor activators of NF-κB, nuclear factor of activated T cells, cytoplasmic 1, cathepsin K, and tartrate-resistant acid phosphatase (TRAP), as well as decreasing the number of TRAP-positive multinucleated cells and hydroxyapatite resorption pits. Furthermore, we showed that irisin suppressed the NF-κB signaling pathway, but activated the p38 and JNK signaling pathways. In the presence of an inhibitor of p38 and JNK, irisin-induced promotion of RAW264.7 cell proliferation was attenuated. However, irisin-induced inhibition of osteoclast differentiation was not affected by either the p38 or JNK signaling pathway. Our study suggested the direct effect of irisin on osteoclastogenesis and revealed the mechanism responsible for the therapeutic potential of irisin in bone metabolism disease.-Ma, Y., Qiao, X., Zeng, R., Cheng, R., Zhang, J., Luo, Y., Nie, Y., Hu, Y., Yang, Z., Zhang, J., Liu, L., Xu, W., Xu, C. C., Xu, L. Irisin promotes proliferation but inhibits differentiation in osteoclast precursor cells.

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Section: Discussionmentioning

confidence: 98%

Irisin promotes proliferation but inhibits differentiation in osteoclast precursor cells

et al. 2018

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“…Osteoporosis is known to be affected by numerous factors, including ethnic origin, socioeconomic status, diet, physical activity, lifestyle, alcohol and drug abuse, and insufficient exposure to sunlight. 1 , 2 Moreover, postmenopausal osteoporosis is associated with adolescent pregnancy, number and frequency of parity, and duration and frequency of lactation. 2 The mainstay diagnosis of osteoporosis is based on the assessment of BMD at the femoral neck and the anterior–posterior lumbar spine using dual-energy X-ray absorptiometry (DXA).…”

Section: Introductionmentioning

confidence: 99%

“…1 , 2 Moreover, postmenopausal osteoporosis is associated with adolescent pregnancy, number and frequency of parity, and duration and frequency of lactation. 2 The mainstay diagnosis of osteoporosis is based on the assessment of BMD at the femoral neck and the anterior–posterior lumbar spine using dual-energy X-ray absorptiometry (DXA). 1 , 2 A T-score ≤−2.5 standard deviation (SD) is considered osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

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Is grand multiparity a risk factor for the development of postmenopausal osteoporosis?

Peker¹,

Tosun²

2018

CIA

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ObjectiveIn this study, we investigated the relationship between the development of postmenopausal osteoporosis and parity.Materials and methodsThe retrospective study included 129 postmenopausal women who were divided into three groups depending on the number of parity: Group I, <5; Group II, 5–9; and Group III, ≥10. The mean age of the subjects was 57.71±5.02 years.ResultsNo significant difference was found among the three groups regarding body mass index values, duration of menopause, mean thyroid stimulating hormone values and frequency of diabetes. Among the three groups, no significant difference was found in terms of the frequency of lumbar osteoporosis (p>0.05), whereas a significant difference was found regarding the frequency of femoral osteoporosis (p=0.012; p<0.05).ConclusionIt was revealed that femoral bone mineral density significantly decreased as the number of parity increased.

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“…Moreover, evidence suggests that irisin also contributes to bone metabolism (4)(5)(6). Both bone mass and bone quality of mice, with or without osteoporosis, were improved after irisin treatment (7).…”

Section: Introductionmentioning

confidence: 99%

The effect of His-tag and point mutation on the activity of irisin on MC3T3-E1 cells

Zeng

Qiao

Zhang

et al. 2018

BST

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Irisin is a myokine secreted from the cleavage of fibronectin type III domain-containing protein 5 (FNDC5) and has an effect on bone formation. There are limited studies about the structure of irisin and its functional unit. In order to clarify the candidate domain responsible for irisin action, we constructed several irisin variants and tested their influence on the proliferation and osteogenesis of MC3T3-E1 cells. On the one hand, His-tag was added to the N terminal or C terminal of irisin. On the other hand, the flexible region or salt bridge site were chosen as the candidate for point mutation. Alkaline phosphatase (ALP), Runt related transcription factor 2 (Runx2) and collagen type I alpha 1 (COL1α1) were chosen to test the differentiation efficiency. We found point mutation on flexible regions, Glu-57 and Ile-107, and adding His-tag on the C-terminal of irisin did affect its action. The osteogenic potential of irisin E57K, irisin I107F and irisin C-His decreased about 90.1%, 88.8% and 96.6% activity of recombinant-irisin (r-irisin) (P < 0.05), respectively. Point mutation on the salt bridge, Arg-75, partly decreased the effect of irisin (45 ± 11.3% of r-irisin) (P < 0.05). N-terminal His-tag showed almost no effect (93.5 ± 25.7% of r-irisin) (P = 0.658). This study suggested that the flexible region of residues 55-58 and 106-108, and C-terminal of irisin are vital for its activity. Disrupting the dimerization of irisin might result in a partly reduced effect on cell differentiation.

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Postmenopausal Osteoporosis Is Associated with Serum Chemerin and Irisin but Not with Apolipoprotein M Levels

Cited by 29 publications

References 12 publications

Irisin promotes proliferation but inhibits differentiation in osteoclast precursor cells

Irisin promotes proliferation but inhibits differentiation in osteoclast precursor cells

Is grand multiparity a risk factor for the development of postmenopausal osteoporosis?

The effect of His-tag and point mutation on the activity of irisin on MC3T3-E1 cells

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