Postmortem cryosectioning as an anatomic reference for human brain mapping

Toga, Arthur W.; Goldkorn, Amir; Ambach, Karen; Chao, Kuo Ching; Quinn, Bruce; Yao, Patrick

doi:10.1016/s0895-6111(96)00072-9

Cited by 36 publications

(29 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, rapid freezing of whole tissue sections might be an alternative approach to chemical methods of fixation. Cryosectioning was demonstrated for the visible human project (14) and for sectioning excised human and animal brains (15)(16)(17). A disadvantage of freezing is that cellular morphology is degraded.…”

Section: Discussionmentioning

confidence: 99%

Three‐dimensional method for comparing in vivo interventional MR images of thermally ablated tissue with tissue response

Breen

Lancaster

Lazebnik

et al. 2003

Magnetic Resonance Imaging

View full text Add to dashboard Cite

Purpose:To investigate the ability of magnetic resonance (MR) to monitor radio-frequency (RF) ablation treatments by comparing MR images of thermal lesions to histologically assayed cellular damage. We developed a new methodology using three-dimensional registration for making spatial correlations. Materials and Methods:A low-field, open MRI system was used to guide an ablation probe into rabbit thigh muscle and acquire MR volumes after ablation. After fixation, we sliced and photographed the tissue at 3-mm intervals, using a specially designed apparatus, to obtain a volume of tissue images. Histologic samples were digitized using a video microscopy system. For our three-dimensional registration method, we used the tissue images as the reference, and registered histology and MR images to them using two different computer alignment steps. First, the MR volume was aligned to the volume of tissue images by registering needle fiducials placed near the tissue of interest. Second, we registered the histology images with the tissue images using a two-dimensional warping technique that aligned internal features and the outside boundary of histology and tissue images. Results:The MR and histology images were very well aligned, and registration accuracy, determined from displacement of needle fiducials, was 1.32 Ϯ 0.39 mm (mean Ϯ SD), which compared favorably to the MR voxel dimensions (0.70 mm in-plane and 3.0 mm thick). A preliminary comparison of MR and tissue response showed that the region inside the elliptical hyperintense rim in MR closely corresponds to the region of necrosis as established by histology, with a mean absolute distance between MR and histology boundaries of 1.17 mm, slightly smaller than the mean registration error. The MR region slightly overestimated the region of necrosis, with a mean signed distance between boundaries of 0.85 mm. Conclusion:Our results suggest that our methodology can be used to achieve three-dimensional registration of histology and in vivo MR images. In MR lesion images, the inner border of the hyperintense region corresponds to the border of irreversible cell damage. This is good evidence that during RF ablation treatments, iMRI lesion images can be used for real-time feedback.

show abstract

Section: Discussionmentioning

confidence: 99%

Three‐dimensional method for comparing in vivo interventional MR images of thermally ablated tissue with tissue response

Breen

Lancaster

Lazebnik

et al. 2003

Magnetic Resonance Imaging

View full text Add to dashboard Cite

show abstract

“…High-resolution anatomical datasets serve as references for the accurate interpretation of clinical data from the PET, computed tomography (CT) and MRI modalities as well as the mapping of transmitters, their receptors (¢gure 8) and other regional biological characteristics. Thus, we have designed a system of histological and digital processing protocols for the acquisition of high resolution, digital imagery from post-mortem cryosectioned whole human brain and head for computer-based, three-dimensional representation and visualization (Cannestra et al 1997;Toga et al 1997). High-resolution (1024 2 pixel) serial images can be captured directly from a cryoplaned blockface using an integrated colour digital camera and ¢bre-optic illumination system mounted over a modi¢ed cryomacrotome.…”

Section: (B) Post-mortem Cryosectioned Materials (I) Data Acquisitionmentioning

confidence: 99%

A probabilistic atlas and reference system for the human brain: International Consortium for Brain Mapping (ICBM)

Mazziotta

Toga²,

Evans

et al. 2001

Phil. Trans. R. Soc. Lond. B

2,325

1,753

View full text Add to dashboard Cite

Motivated by the vast amount of information that is rapidly accumulating about the human brain in digital form, we embarked upon a program in 1992 to develop a four-dimensional probabilistic atlas and reference system for the human brain. Through an International Consortium for Brain Mapping (ICBM) a dataset is being collected that includes 7000 subjects between the ages of eighteen and ninety years and including 342 mono-and dizygotic twins. Data on each subject includes detailed demographic, clinical, behavioural and imaging information. DNA has been collected for genotyping from 5800 subjects. A component of the programme uses post-mortem tissue to determine the probabilistic distribution of microscopic cyto-and chemoarchitectural regions in the human brain. This, combined with macroscopic information about structure and function derived from subjects in vivo, provides the ¢rst large scale opportunity to gain meaningful insights into the concordance or discordance in micro-and macroscopic structure and function. The philosophy, strategy, algorithm development, data acquisition techniques and validation methods are described in this report along with database structures. Examples of results are described for the normal adult human brain as well as examples in patients with Alzheimer's disease and multiple sclerosis. The ability to quantify the variance of the human brain as a function of age in a large population of subjects for whom data is also available about their genetic composition and behaviour will allow for the ¢rst assessment of cerebral genotype^phenotype^behavioural correlations in humans to take place in a population this large. This approach and its application should provide new insights and opportunities for investigators interested in basic neuroscience, clinical diagnostics and the evaluation of neuropsychiatric disorders in patients.

show abstract

“…A 3-D registration from the reconstructed volume with a reference MRI was done using a similar block matching strategy. Toga et al [13] used a fiducial marker based registration process to align serial sections. Their method uses a combination of local and differential scaling to put anatomical data in Talairach space [12].…”

Section: Introductionmentioning

confidence: 99%

The Creation of a Brain Atlas for Image Guided Neurosurgery Using Serial Histological Data

Chakravarty

Bertrand

Descouteaux

et al. 2003

Lecture Notes in Computer Science

122

View full text Add to dashboard Cite

Abstract. Digital atlases of the human brain can help in the specific localization of structures of surgical relevance and interest in Image Guided Neurosurgery (IGNS). This paper outlines one of the steps in the creation of a digital atlas intended for IGNS, using histological data. The acquisition of histological data can include artefacts such as tearing, shearing, stretching, shrinking, as well as inhomogeneous staining leading to structural inhomogeneities. These inconsistencies are reduced using a non-linear intensity based registration procedure where deformations are defined using a maximized correlation coefficient estimate. The intensity artefacts brought about by inhomogeneous staining are reduced by applying a slice to slice intensity inhomogeneity correction by modelling the intensity mapping between slices as a third order polynomial that is estimated with a Least Trimmed Squared fit. The lateral ventricle was then segmented and to demonstrate increased smoothing along its surface.

show abstract

Postmortem cryosectioning as an anatomic reference for human brain mapping

Cited by 36 publications

References 12 publications

Three‐dimensional method for comparing in vivo interventional MR images of thermally ablated tissue with tissue response

Three‐dimensional method for comparing in vivo interventional MR images of thermally ablated tissue with tissue response

A probabilistic atlas and reference system for the human brain: International Consortium for Brain Mapping (ICBM)

The Creation of a Brain Atlas for Image Guided Neurosurgery Using Serial Histological Data

Contact Info

Product

Resources

About