Postnatal changes in the distribution and density of neuronal nuclei and doublecortin antigens in domestic chicks (<i>Gallus domesticus</i>)

Mezey, Szilvia; Krivokuća, Dragan; Bálint, Erika; Adorján, András; Zachár, Gergely; Csillag, András

doi:10.1002/cne.22696

Cited by 26 publications

(41 citation statements)

References 56 publications

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“…NeuN antibody was obtained from Millipore (MAB377; clone A60). Similar cellular expression profiles have been described in mouse and chicken, including a common lack of label in Purkinje cells of the cerebellum (Mezey et al 2012;Weyer and Schilling 2003). Specificity has been verified with Western blots using chicken protein extracted from whole brains which showed specific bands at estimated molecular weights of 46 and 48 kDa (Mezey et al 2012), similar to mouse (Lind et al 2005).…”

Section: Neun Antibody Specificitysupporting

confidence: 63%

“…Specificity has been verified with Western blots using chicken protein extracted from whole brains which showed specific bands at estimated molecular weights of 46 and 48 kDa (Mezey et al 2012), similar to mouse (Lind et al 2005). NeuN has been used extensively to label postmitotic neurons in developmental studies in chick (Mezey et al 2012;Perez et al 1999;Zeng et al 2008) and in a study of cochlear nucleus neurons in mice (Mostafapour et al 2002).…”

Section: Neun Antibody Specificitymentioning

confidence: 97%

“…In order to identify and count the neuronal population in NA, the pan-neuronal molecular marker NeuN was used (Mezey et al 2012;Mullen et al 1992;Weyer and Schilling 2003). NeuN label was widely observed throughout the chick brain stem, with abundant labeling in the auditory areas NA and NM, as well as the target nucleus for NM involved in interaural timing difference coding, nucleus laminaris (NL; Fig.…”

Section: Neun Expression In the Avian Auditory Brainstemmentioning

confidence: 99%

“…Originally identified as a neuronal specific nuclear protein in mouse (Mullen et al 1992), NeuN has been used as a pan-neuronal marker in a number of studies of chick neuroanatomy and developmental studies in a variety of tissues, including forebrain, optic tectum, and spinal cord (Mezey et al 2012;Perez et al 1999;Yamagata et al 2006;Zeng et al 2008). While antiNeuN labeled most neurons, including those in the murine cochlear nucleus (Mostafapour et al 2002), it was notably absent in a few specific cell types: Purkinje cells in the cerebellum, mitral cells in the olfactory bulb, and photoreceptor neurons in the retina (Lind et al 2005;Mullen et al 1992;Weyer and Schilling 2003).…”

Section: Neun Expression In the Chick Auditory Brain Stemmentioning

confidence: 99%

“…Differential subcellular localization of NeuN label in NA may also be related to the finding that two isoforms of NeuN (molecular weights 46 and 48 kDa) are present in both mice and chick and both are labeled by the NeuN antibody (Lind et al 2005;Mezey et al 2012). In mice, these isoforms differ in their subcellular distributions, with the 48 kDa protein preferentially expressed in the cytoplasm, but whether differential isoform expression explains the heterogeneity in subcellular labeling in NA remains to be determined.…”

Section: Neun Expression In the Chick Auditory Brain Stemmentioning

confidence: 99%

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Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Bloom

Williams

MacLeod

2014

JARO

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Multiple calcium-binding proteins (CaBPs) are expressed at high levels and in complementary patterns in the auditory pathways of birds, mammals, and other vertebrates, but whether specific members of the CaBP family can be used to identify neuronal subpopulations is unclear. We used double immunofluorescence labeling of calretinin (CR) in combination with neuronal markers to investigate the distribution of CR-expressing neurons in brainstem sections of the cochlear nucleus in the chicken (Gallus gallus domesticus). While CR was homogeneously expressed in cochlear nucleus magnocellularis, CR expression was highly heterogeneous in cochlear nucleus angularis (NA), a nucleus with diverse cell types analogous in function to neurons in the mammalian ventral cochlear nucleus. To quantify the distribution of CR in the total NA cell population, we used antibodies against neuronal nuclear protein (NeuN), a postmitotic neuronspecific nuclear marker. In NA neurons, NeuN label was variably localized to the cell nucleus and the cytoplasm, and the intensity of NeuN immunoreactivity was inversely correlated with the intensity of CR immunoreactivity. The percentage of CR+neurons in NA increased from 31 % in embryonic (E)17/18 chicks, to 44 % around hatching (E21), to 51 % in postnatal day (P) 8 chicks. By P8, the distribution of CR + neurons was uniform, both rostrocaudal and in the tonotopic (dorsoventral) axis.Immunoreactivity for the voltage-gated potassium ion channel Kv1.1, used as a marker for physiological type, showed broad and heterogeneous postsynaptic expression in NA, but did not correlate with CR expression. These results suggest that CR may define a subpopulation of neurons within nucleus angularis.

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Section: Neun Antibody Specificitysupporting

confidence: 63%

Section: Neun Antibody Specificitymentioning

confidence: 97%

Section: Neun Expression In the Avian Auditory Brainstemmentioning

confidence: 99%

Section: Neun Expression In the Chick Auditory Brain Stemmentioning

confidence: 99%

Section: Neun Expression In the Chick Auditory Brain Stemmentioning

confidence: 99%

See 3 more Smart Citations

Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Bloom

Williams

MacLeod

2014

JARO

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Endogenous versus exogenous markers of adult neurogenesis in canaries and other birds: Advantages and disadvantages

Balthazart

Ball

2014

J of Comparative Neurology

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Although the existence of newborn neurons had originally been suggested, but not broadly accepted, based on studies in adult rodent brains, the presence of an active neurogenesis process in adult homoeothermic vertebrates was first firmly established in songbirds. Adult neurogenesis was initially studied with the tritiated thymidine technique, later replaced by the injection and detection of the marker of DNA replication 5-bromo-2′-deoxyuridine (BrdU). More recently, various endogenous markers were used to identify young neurons or cycling neuronal progenitors. We review here the respective advantages and pitfalls of these different approaches in birds, with specific reference to the microtubule-associated protein, doublecortin (DCX), that has been extensively used to identify young newly born neurons in adult brains. All these techniques of course have limitations. Exogenous markers label cells replicating their DNA only during a brief period and it is difficult to select injection doses that would exhaustively label all these cells without inducing DNA damage that will also result in some form of labeling during repair. On the other hand, specificity of endogenous markers is difficult to establish due to problems related to the specificity of antibodies (these problems can be, but are not always, addressed) and more importantly because it is difficult, if not impossible, to prove that a given marker exhaustively and specifically labels a given cell population. Despite these potential limitations, these endogenous markers and DCX staining in particular clearly represent a useful approach to the detailed study of neurogenesis especially when combined with other techniques such as BrdU.

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Olfactory placode generates a diverse population of neurons expressing GnRH, somatostatin mRNA, neuropeptide Y, or calbindin in the chick forebrain

Murakami

Ohki‐Hamazaki

Uchiyama

2022

J of Comparative Neurology

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The olfactory placode (OP) of vertebrates generates several classes of migrating cells, including hypothalamic gonadotropin-releasing hormone (GnRH)-producing neurons, which play essential roles in the reproduction system. Previous studies using OP cell labeling have demonstrated that OP-derived non-GnRH cells enter the developing forebrain; however, their final fates and phenotypes are less well understood. In chick embryos, a subpopulation of migratory cells from the OP that is distinct from GnRH neurons transiently expresses somatostatin (SS). We postulated that these cells are destined to develop into brain neurons. In this study, we examined the expression pattern of SS mRNA in the olfactory-forebrain region during development, as well as the destination of OP-derived migratory cells, including SS mRNA-expressing cells. Utilizing the Tol2 genomic integration system to induce long-term fluorescent protein expression in OP cells, we found that OP-derived migratory cells labeled at embryonic day (E) 3 resided in the olfactory nerve and medial forebrain at E17-19. A subpopulation of green fluorescent protein (GFP)-labeled GnRH neurons that remained in the olfactory nerve was considered to comprise terminal nerve neurons. In the forebrain, GFP-labeled cells showed a distribution pattern similar to that of GnRH neurons. A large proportion of GFP-labeled cells expressed the mature neuronal marker NeuN.Among the GFP-labeled cells, the percentage of GnRH neurons was low, while the remaining GnRH-negative neurons either expressed SS mRNA, neuropeptide Y, or calbindin D-28k or did not express any of them. These results indicate that a diverse population of OP-derived neuronal cells, other than GnRH neurons, integrates into the chick medial forebrain.

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Postnatal changes in the distribution and density of neuronal nuclei and doublecortin antigens in domestic chicks (Gallus domesticus)

Cited by 26 publications

References 56 publications

Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Endogenous versus exogenous markers of adult neurogenesis in canaries and other birds: Advantages and disadvantages

Olfactory placode generates a diverse population of neurons expressing GnRH, somatostatin mRNA, neuropeptide Y, or calbindin in the chick forebrain

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