Postnatal development of the rat organ of Corti

Roth, Birgit; Bruns, Volkmar

doi:10.1007/bf00185615

Cited by 65 publications

(16 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known also that the OHCs from different regions of the cochlea develop differently. Basal OHCs reach adult-like size at PD3, whereas apical OHCs continue to grow in length up to PD16 (Roth and Volkmar, 1992;Mu et al, 1997). A similar base-to-apex pattern of development was observed in the expression of the putative OHC water channel proteins.…”

Section: Postnatal Development Of Water Permeability and Electromotilitymentioning

confidence: 54%

Water Permeability of Cochlear Outer Hair Cells: Characterization and Relationship to Electromotility

et al. 2000

View full text Add to dashboard Cite

The distinguishing feature of the mammalian outer hair cells (OHCs) is to elongate and shorten at acoustic frequencies, when their intracellular potential is changed. This "electromotility" or "electromechanics" depends critically on positive intracellular pressure (turgor), maintained by the inflow of water through yet uncharacterized water pathways. We measured the water volume flow, J v , across the plasma membrane of isolated guinea pig and rat OHCs after osmotic challenges and estimated the osmotic water permeability coefficient, P f , to be ϳ10 Ϫ 2 cm/sec. This value is within the range reported for osmotic flow mediated by the water channel proteins, aquaporins. J v was inhibited by HgCl 2 , which is known to block aquaporin-mediated water transport. P f values that were estimated for OHCs from neonatal rats were of the order of ϳ2ϫ10 Ϫ 3 cm/sec, equivalent to that of membranes lacking water channel proteins. In an immunofluorescence assay we showed that an anti-peptide antibody specific for aquaporins labels the lateral plasma membrane of the OHC in the region in which electromotility is generated. Using patch-clamp recording, we found that water influx into the OHC is regulated by intracellular voltage. We also found that the most pronounced increases of the electromotility-associated charge movement and of the expression of OHC water channels occur between postnatal days 8 and 12, preceding the onset of hearing function in the rat. Our data indicate that electromotility and water transport in OHCs may influence each other structurally and functionally.

show abstract

Section: Postnatal Development Of Water Permeability and Electromotilitymentioning

confidence: 54%

Water Permeability of Cochlear Outer Hair Cells: Characterization and Relationship to Electromotility

et al. 2000

View full text Add to dashboard Cite

show abstract

“…DPOAE measurements were only performed on rats whose auditory system was mature (Lenoir et al, 1986(Lenoir et al, , 1987Roth and Bruns, 1992;Henley et al, 1989). In addition, noise and styrene exposure were initiated beyond the critical period during which rats' ears are particularly susceptible to damage by noise (Rybalko and Syka, 2001) or ototoxic agents (Henley and Rybak, 1995).…”

Section: Discussionmentioning

confidence: 99%

Impact of noise or styrene exposure on the kinetics of presbycusis

et al. 2011

View full text Add to dashboard Cite

“…The data presented in this study are from the mid-turn of the cochlea to avoid the basal to apical developmental gradient [4,74-77]. …”

Section: Methodsmentioning

confidence: 99%

Synaptic profiles during neurite extension, refinement and retraction in the developing cochlea

et al. 2012

View full text Add to dashboard Cite

BackgroundDuring development, excess synapses form between the central and peripheral nervous systems that are then eliminated to achieve correct connectivity. In the peripheral auditory system, the developing type I spiral ganglion afferent fibres undergo a dramatic re-organisation, initially forming connections with both sensory inner hair cells (IHCs) and outer hair cells (OHCs). The OHC connections are then selectively eliminated, leaving sparse innervation by type II afferent fibres, whilst the type I afferent synapses with IHCs are consolidated.ResultsWe examined the molecular makeup of the synaptic contacts formed onto the IHCs and OHCs during this period of afferent fibre remodelling. We observed that presynaptic ribbons initially form at all the afferent neurite contacts, i.e. not only at the expected developing IHC-type I fibre synapses but also at OHCs where type I fibres temporarily contact. Moreover, the transient contacts forming onto OHCs possess a broad set of pre- and postsynaptic proteins, suggesting that functional synaptic connections are formed prior to the removal of type I fibre innervation. AMPA-type glutamate receptor subunits were transiently observed at the base of the OHCs, with their downregulation occurring in parallel with the withdrawal of type I fibres, dispersal of presynaptic ribbons, and downregulation of the anchoring proteins Bassoon and Shank. Conversely, at developing type I afferent IHC synapses, the presence of pre- and postsynaptic scaffold proteins was maintained, with differential plasticity in AMPA receptor subunits observed and AMPA receptor subunit composition changing around hearing onset.ConclusionsOverall our data show a differential balance in the patterns of synaptic proteins at developing afferent IHC versus OHC synapses that likely reflect their stable versus transient fates.

show abstract

Postnatal development of the rat organ of Corti

Cited by 65 publications

References 25 publications

Water Permeability of Cochlear Outer Hair Cells: Characterization and Relationship to Electromotility

Water Permeability of Cochlear Outer Hair Cells: Characterization and Relationship to Electromotility

Impact of noise or styrene exposure on the kinetics of presbycusis

Synaptic profiles during neurite extension, refinement and retraction in the developing cochlea

Contact Info

Product

Resources

About