Postulation of leaf rust resistance genes in Indian and exotic wheat germplasm using near-isogenic lines (NILs) and molecular markers

Kaur, Harmandeep; Kaur, Jaspal; Bala, Ritu; Sharma, Achla; Kumari, Jyoti; Mavi, G.S.; Kaur, Satinder

doi:10.1016/j.cropro.2023.106431

Cited by 2 publications

(2 citation statements)

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“…Pal, and since then, the rapidly evolving fungal pathogens have never let a pause in resistance breeding. Both the rusts have been identified with multiple variants such as in LR, the groups named 12, 77 and 104 have repeatedly evolved, resulting in the breakdown of many important LR resistance genes [ 36 , 46 ]. Similarly, virulence against important YR resistance genes such as Yr9, Yr17, Yr18, Yr19, Yr21, Yr22, Yr23, Yr25, Yr27 have been detected in newly evolved pathotypes 238S119, 110S119 and 46S119 of yellow rust [ 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…Seedlings of parental lines and mapping population were tested against prevalent Puccinia triticina ( Pt ) pathotype 77−9 (121R60-1) having avirulence/virulence formula: Lr2a, Lr2b, Lr2c, Lr9, Lr19, Lr24, Lr25, Lr28, Lr32, Lr39, Lr42, Lr45, Lr47 / Lr1, Lr3, Lr10, Lr11, Lr12, Lr13, Lr14a, Lr14b, Lr14ab, Lr15, Lr16, Lr17a, Lr17b, Lr18, Lr20, Lr21, Lr22a, Lr22b, Lr23, Lr26, Lr27 + 31, Lr30, Lr33, Lr34, Lr35, Lr36, Lr37, Lr38, Lr44, Lr46, Lr48, Lr49 [ 36 ]. Seeds were sown in rows in plastic boxes (25 cm × 10 cm), with 8 rows/box and 10–12 seeds per row, with the first row as susceptible check WL711 in each box.…”

Section: Methodsmentioning

confidence: 99%

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Mapping of Aegilops speltoides derived leaf rust and stripe rust resistance genes using 35K SNP array

Kaur,

Bai,

Dhillon

et al. 2024

BMC Genom Data

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Wheat is an essential food commodity cultivated throughout the world. However, this crop faces continuous threats from fungal pathogens, leaf rust (LR) and stripe rust (YR). To continue feeding the growing population, these major destructors of wheat must be effectively countered by enhancing the genetic diversity of cultivated germplasm. In this study, an introgression line with hexaploid background (ILsp3603) carrying resistance against Pt pathotypes 77−5 (121R63-1), 77−9 (121R60-1) and Pst pathotypes 46S119 (46E159), 110S119 (110E159), 238S119 (238E159) was developed from donor wheat wild progenitor, Aegilops speltoides acc pau 3603. To understand the genetic basis of resistance and map these genes (named Lrsp3603 and Yrsp3603), inheritance studies were carried out in F6 and F7 mapping population, developed by crossing ILsp3603 with LR and YR susceptible cultivar WL711, which revealed a monogenic (single gene) inheritance pattern for each of these traits. Bulk segregant analysis combined with 35 K Axiom SNP array genotyping mapped both genes as separate entities on the short arm of chromosome 6B. A genetic linkage map, comprising five markers, 1 SNP, 1 PLUG and three gene based SSRs, covered a genetic distance of 12.65 cM. Lrsp3603 was flanked by markers Tag-SSR14 (located proximally at 2.42 cM) and SNP AX-94542331 (at 3.28 cM) while Yrsp3603 was mapped at one end closest to AX-94542331 at 6.62 cM distance. Functional annotation of Lrsp3603 target region (∼ 1 Mbp) revealed 10 gene IDs associated with disease resistance mechanisms including three encoding typical R gene domains.

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