Potato leafroll virus (PLRV): its transmission and control

Syller, J.

doi:10.1007/bf00139765

Cited by 33 publications

(13 citation statements)

References 117 publications

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“…Potato leafroll virus (PLRV), family Luteoviridae, genus Polerovirus, is persistently transmitted by aphids in a nonpropagative manner and has a limited host range of approximately 20 species, mostly of the Solanaceae (Syller 1996;Taliansky et al 2003), and is a primary virus pathogen of Electronic supplementary material The online version of this article (doi:10.1007/s12230-016-9496-6) contains supplementary material, which is available to authorized users. potato.…”

Section: Introductionmentioning

confidence: 99%

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection

et al. 2016

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Potato leafroll virus (PLRV) is a major pathogen of potato with worldwide impact on seed and commercial production of potato. In North America, the primary potato cultivars grown by industry are not resistant to PLRV and require the application of insecticides to control the aphid vector of PLRV, so as to prevent PLRV infection of the crop. The Solanum etuberosum-derived dominant gene Rlr etb confers resistance to PLRV and has been mapped to chromosome 4. The previous closest marker to Rlr etb , C2_At1g42990, was mapped 13.6 cM from the gene in a BC 3 population. Cleaved amplified polymorphic sequence markers were developed in the region surrounding C2_At1g42990 and mapped along with Rlr etb in 102 BC 4 progeny. Sixteen markers were identified surrounding Rlr etb , with flanking markers at 2.1 and 9.3 cM. Two markers, 1367-8a and DMB32-11, both 2.1 cM from Rlr etb , are shown to be well suited for marker assisted selection. The development of additional molecular markers more closely linked to Rlr etb will greatly facilitate its use in potato breeding and its continued introgression into cultivated potato for the development of PLRV-resistant cultivars.Resumen El virus del enrollamiento de la hoja de la papa (PLRV) es un patógeno importante de la papa con impacto mundial en la producción de semilla y en la producción comercial de la papa. En Norteamérica, las principales variedades de papa que se cultivan por la industria no son resistentes al PLRV y requieren de la aplicación de insecticidas para control del áfido vector del PLRV, así como para prevenir la infección del cultivo por PLRV. El gen dominante derivado de Solanum etuberosum, Rlretb, confiere resistencia al PLRV y ha sido ubicado en el cromosoma 4. El marcador previo más cercano al Rlretb, C2_At1g42990, fue ubicado a 13.6 cM del gen en una población BC3. Se desarrollaron marcadores fragmentados amplificados de secuencias polimórficas en la región que rodea a C2_At1g42990 y se les hizo un mapa junto con Rlretb en una progenie 102 BC4. Se identificaron 16 marcadores rodeando al Rlretb, con marcadores en los lados a 2.1 y 9.3 cM. Dos marcadores, 1367-8a y DMB32-11, ambos a 2.1 cM de Rlretb, se ha demostrado que son los apropiados para la selección asistida por marcadores. El desarrollo de marcadores moleculares adicionales más cercanamente ligados a Rlretb facilitará grandemente su uso en el mejoramiento de la papa así como su incorporación a papa cultivada para el desarrollo de variedades resistentes al PLRV.

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Section: Introductionmentioning

confidence: 99%

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection

et al. 2016

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“…PLRV damages of the potato crop are controlled by the use of certified seed (Radcliffe and Ragsdale 2002) and by insecticide applications (Mowry 2005). However, these techniques are not always accessible, may be also costly, in particular to small-farmers, and produce environmental and human health concerns (Paoletti and Pimentel 2000; Syller 1996; Thomas et al 1997). …”

Section: Introductionmentioning

confidence: 99%

Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi

et al. 2016

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An inverted repeat construct corresponding to a segment of the potato leaf roll virus coat protein gene was created under control of a constitutive promoter and transferred into a transformation vector with a heat inducible Cre-loxP system to excise the nptII antibiotic resistance marker gene. Fifty-eight transgenic events were evaluated for resistance to PLRV by greenhouse inoculations, which lead to the identification of 7 highly resistant events, of which 4 were extremely resistant. This resistance was also highly effective against accumulation in subsequent tuber generations from inoculated plants, which has not been reported before. Northern blot analysis showed correlation of PLRV specific siRNA accumulation with the level of PLRV resistance. Heat mediated excision of the nptII antibiotic resistance gene in PLRV resistant events was highly efficient in one event with full excision in 71 % of treated explants. On the other hand 8 out of 10 analyzed events showed truncated T-DNA insertions lacking one of the two loxP sites as determined by PCR and confirmed by sequencing flanking regions in 2 events, suggesting cryptic LB sites in the non-coding region between the nptII gene and the flanking loxP site. Accordingly, it is proposed to modify the Cre-loxP vector by reducing the 1 kb size of the region between nptII, loxP, and the LB.Electronic supplementary materialThe online version of this article (doi:10.1007/s11248-016-9976-y) contains supplementary material, which is available to authorized users.

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“…Potato leafroll virus (PLRV) is one of the most significant and widespread viral pathogens of potato (Syller 1996;Barker and Waterhouse 1999). Besides reduction of total tuber yield, infection with PLRV can lead to losses in marketable yield due to the expression of net necrosis in tubers (Novy et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Two allelic or tightly linked genetic factors at the PLRV.4 locus on potato chromosome XI control resistance to potato leafroll virus accumulation

et al. 2004

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A novel locus for potato resistance to potato leafroll virus (PLRV) was characterized by inheritance studies and molecular mapping. The diploid parental clone DW 91-1187 was resistant to PLRV accumulation in both inoculated plants and their tuber progeny. The resistance to PLRV accumulation present in DW 91-1187 was not transmitted to any F 1 offspring when crossed with a PLRV susceptible clone. Instead, one half of the F 1 individuals exhibited undetectable amounts of PLRV as determined by ELISA during the primary infection assay, but accumulated PLRV in their tuber progeny plants. The other half was clearly infected both in the inoculated and tuber-born plants. The inheritance of resistance to PLRV accumulation may be explained by a model of two complementary alleles of a single gene (PLRV.4) or by two complementary genes that are closely linked in repulsion phase. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers linked to the PLRV.4 locus were selected. The two complementary factors were closely linked in coupling phase to the alternative alleles UBC864 600 and UBC864 800 of DNA marker UBC864. These markers may be used for marker-assisted selection of genotypes having both factors for resistance to PLRV accumulation. The PLRV.4 locus was mapped to a central position on linkage group XI of the potato molecular map, where no resistance locus has been mapped previously.

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Potato leafroll virus (PLRV): its transmission and control

Cited by 33 publications

References 117 publications

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection

Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi

Two allelic or tightly linked genetic factors at the PLRV.4 locus on potato chromosome XI control resistance to potato leafroll virus accumulation

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