Potensi Kolagen Teripang Emas Sebagai Inhibitor Tirosinase

Abstract

Kolagen memiliki aktivitas antioksidan dan mampu menghambat aktivitas tirosinase pada proses<br />melanogenesis, salah satu biota laut yang dapat dijadikan sumber kolagen alternatif adalah teripang.<br />Penelitian ini bertujuan menentukan karakteristik kolagen dan aktivitas penghambatan enzim tirosinase<br />oleh kolagen teripang emas (Stichopus hermanii). Penghilangan protein non kolagen dilakukan dengan<br />cara perendaman daging teripang menggunakan NaOH 0,1 M selama 48 jam. Isolasi kolagen dilakukan<br />menggunakan asam asetat 0.5 M selama 48 jam. Analisis gugus fungsi kolagen dilakukan menggunakan<br />FTIR dan analisis aktivitas tirosinase dilakukan menggunakan spektrofotometer. Kadar protein non<br />kolagen yang diperoleh yaitu 0,090 mg/mL dan kolagen teripang emas memiliki rendemen 0,66%. Kolagen<br />teripang memiliki nilai pH 6,91, gugus fungsi khas amida A (3379,29 cm-1), amida B (2924,09 cm-1), amida<br />I (1681,93 cm-1), amida II (1568,13 cm-1), dan amida III (1269,16 cm-1). Aktivitas penghambatan tirosinase<br />menunjukkan bahwa kolagen teripang emas memiliki nilai IC50 5610 ppm. Kolagen teripang emas belum<br />mampu menghambat aktivitas tirosinase.

Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

“…Other research showed that dried collagen extracted from S. vastus and H. atra produced yield of 0.92% and 0.88% wb (Yuniati & Sulardiono, 2019). Meanwhile, the dried collagen extracted from golden sea cucumber (S. hermanii) had a lower yield, i.e., 0.66% (Safithri et al, 2018). In this research, the dialyzed collagen was Figure.1.…”

“…This result supported Purcell et al (2012) findings that S. horrens, S. monotuberculatus, S. quadrifasciatus, and S. naso are often indistinguishable due to their similar appearances. The local name golden sea cucumber or "teripang emas" are commonly known as S. variegatus which are found in some publications (Adriansyah & Sulastri, 2014;Cahyati et al, 2018) or S. hermanni (Arundina et al, 2015;Damaiyanti, 2015;Safithri et al, 2018;Susanto, Savitrim, & Tarman, 2018). However, it was also reported that the S. hermanni was previously known as S. variegatus (Pangestuti & Arifin, 2018).…”

“…Golden sea cucumber belongs to the genus Stichopus, which can be found in Sumatra, Nusa Tenggara and Sulawesi waters (Fad'ha, Arma, & Busman, 2019;Nursid, Marraskuranto, Kuswardini, & Winanto, 2019;Pangestuti et al, 2016;Safithri, Setyaningsih, Tarman, Yuhendri, & Meydia, 2018). To date, golden sea cucumbers are often called Stichopus hermanni or S. variegatus because of their similar morphological characteristics.…”

“…Research on the biological activity of sea cucumbers from Nusa Tenggara have been reported, including radical scavenging activity of ethanolic extract of Holothuria leucospilota, H. atra, H. fuscocinerea, and H. excellens (Pangestuti et al, 2016), -glucosidase inhibition of methanolic extract of S.hermanni (Mariyanti, 2017), and tyrosinase inhibitor activity of S. hermanni collagen (Safithri et al, 2018). Other explorations of golden sea cucumber or gamat (S. hermanni or S. variegatus) from various locations that have been published were studies on collagen extraction (Alhana, Suptijah, & Tarman, 2015;Fawzya, Chasanah, Poernomo, & Khirzin, 2016), sulfated glycosaminoglycan content (Masre, Yip, Sirajudeen, & Ghazali, 2010), angiotensin-I-converting enzyme (ACE) inhibitor and antioxidant activity of collagen peptide (Khirzin et al, 2015), the potential of S. hermanii to improve the growth of stem cells (Arundina, Suardita, Setiabudi, & Ariani, 2016), the effect of sea cucumber extract on the lymphocytes numbers of Wistar rat's oral mucus during the healing process from a traumatic ulcer (Arundina, Yuliati, Soesilawati, Damaiyanti, & Maharani, 2015); the growth-promoting effect of S. variegatus its water extract to boost the proliferation of spinal astrocytes (Patar, Jamalullail, Jaafar, & Abdullah, 2012) and the ability of sulfated glycosaminoglycan from S. hermanni integument in the process of wound healing (Masre et al, 2010).…”

Karakteristik Kolagen Larut Asam Teripang Gama (Stichopus variegatus)

“…Menurut Purcell (2014) kandungan kolagen yang terdapat dalam teripang mencapai 86% dari total protein pada tubuh teripang. Isolasi kolagen dari berbagai jenis teripang cukup banyak dilakukan, di antaranya isolasi kolagen dari Stichopus japonicas (Cui et al 2007), Stichopus vastus (Abedin et al 2012), dan Stichopus hermanii (Safithri et al 2018).…”