Potential Anti-Tumor and Anti-Inflammatory Activity of Six Mistletoe Plants in the Family Viscaceae Present in Western Ghats, India

Kuttan, Ramadasan; Sivamurthy, R G; Kuttan, Girija

doi:10.22159/ijpps.2017v9i11.20541

Cited by 5 publications

(8 citation statements)

References 28 publications

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“…These biologically active compounds in plant extracts could be an important contributory factor involved in their anticancer potentials. The similar results have been observed on the plants such as Zingiber officinale [17] and Sophora interrupta and the plants from Viscaceae family [18] experimenting in vitro on DLA and EAC cells which can be correlated.…”

Section: Pavani and Naika Asian J Pharm Clinsupporting

confidence: 84%

In Vitro Cytotoxicity Activity of Methanolic Leaf and Fruit Extract of Zanthoxylum Ovalifolium Wight Against Dalton’s Lymphoma Ascites and Ehrlich Ascites Carcinoma Cancer Cell Lines

Pavani

Naika

2019

Asian J Pharm Clin Res

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Objectives: The objective of the present study, the methanolic leaf and fruit extraction of Zanthoxylum ovalifolium, was examined for in vitro cytotoxicity using trypan blue dye exclusion technique. Methodology: In vitro cytotoxicity activity of methanolic leaf and fruit extracts of Z. ovalifolium. Against Dalton’s lymphoma ascites (DLA) and Ehrlich ascites carcinoma (EAC) cell lines evaluated using trypan blue exclusion method. Non-viable cell absorbs blue color of trypan blue while viable cell does not absorb the dye. The percentage of total number of viable and non-viable cells is calculated using % viability=(live cell count/total cell count)×100. Results: Cytotoxicity activity of methanolic leaf and fruit extract against chosen tumor cell line at different concentrations of 10, 20, 50,100, and 200 μg/ml, respectively. The result confirmed that the fruit extract at 200 showed less toxic effect against DLA and EAC cell line showed 72% and 75% of inhibition growth of cell line and the most potent 80% and 82% of cell death were found in leaf extract against the chosen tumor cell line. The present study indicates that both leaf and fruit extract of Z. ovalifolium were appreciable antitumor activity against DLA and EAC cell lines may be due to the presence of phytochemicals such as flavonoids and alkaloids present in the plant. Conclusion: Zanthoxylum ovalifolium wight fruit and leaf methanolic extracts showed satisfying result of cytotoxicity activity against DLA and EAC cell line. Hence, Z.ovalifolium could be suggested as an important source of anticancer agent for pharmacological industries.

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Section: Pavani and Naika Asian J Pharm Clinsupporting

confidence: 84%

In Vitro Cytotoxicity Activity of Methanolic Leaf and Fruit Extract of Zanthoxylum Ovalifolium Wight Against Dalton’s Lymphoma Ascites and Ehrlich Ascites Carcinoma Cancer Cell Lines

Pavani

Naika

2019

Asian J Pharm Clin Res

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“…There is been report that inflammatory response directly related to chronic diseases such as cardiovascular diseases, rheumatoid arthritis, and many types of cancer [21]. There is a direct link between anti-inflammatory activities with the anti-tumor activity [22]. Now, there is a need for alternative therapeutic selection to establish anti-inflammatory drug as they are safe, less toxic, effective, Significance level: The data were analyzed using ANOVA and expressed as Mean±SEM followed by Dunnett's test and differences between means were regarded significant at P<0.015*, P<0.01**, and P<0.001***, Z. ovalifolium: Zanthoxylum ovalifolium, SEM: Standard error of mean…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Properties of Zanthoxylum Ovalifolium Weight Methanolic Extract on Albino Wistar Rats

Pavani

Naika

2019

Asian J Pharm Clin Res

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Objective: The object of the present study, the methanolic leaf extract of Zanthoxylum ovalifolium was examined for anti-inflammatory activity and tested using carrageenan-induced paw edema in albino Wistar rats. Materials and Methods: The rats were grouped into five, each group consisting of six animals, Group I (negative control) received 1 ml of saline, Group II (standard) received 10 mg/kg p. o. of indomethacin, and remaining three groups received three different doses (100, 200, and 400 mg/kg p. o.) of the methanolic leaf extract after 1 h 0.1 ml of 1% carrageenan injection. The result of anti-inflammatory activity was set up to be dose-dependent in the carrageenan-induced paw edema model. Results: The methanolic leaf extract has shown significant (p<0.001) inhibition of paw edema, 66%, 66.03%, and 69.29% on the 3rd h at the doses of 100, 200, and 400 mg/kg, p. o., respectively. The results were expressed as the Mean±standard error of mean and statistical significance was analyzed by ANOVA followed by Dunnett’s test. The methanolic leaf of Z. ovalifolium extract showed significant anti-inflammatory activity compared with the standard drug indomethacin. Conclusion: Methanolic leaf extract of Z.ovalifolium showed excellent results in the anti-inflammatory experiment so, the plant can be explored as a potential medicine in suppressing inflammation.

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“…b.wt) aqueous extract. These doses were fixed after studying the toxicity profile of the extracts (Kuttan et al, 2017). On 6 th day, blood was collected from the caudal vein of all animals and various parameters such as, total white blood cells (WBC) count (Hemocytometer), differential count (Leishman's stain) and hemoglobin level (Cyanmethaemoglobin method) (Cheesbrough and McArthur, 1976) were measured.…”

Section: Determination Of the Effect Of Viscaceae Plant Extract On The Haematological Parameters And Body Weightmentioning

confidence: 99%

“…Not much work has been done in other Viscum species. Recently, we had reported the tumor reducing activity of six plants of Viscum species present in the Western Ghats of India (Kuttan et al, 2017). However, out of six plants, five of them such as Viscum orientale Wild, Viscum nepalense Spiens, Viscum ramosissimum Well, Viscum capitallatum Sm, Viscum trilobatum Talbot were found to be highly toxic.…”

Section: Introductionmentioning

confidence: 99%

“…However, out of six plants, five of them such as Viscum orientale Wild, Viscum nepalense Spiens, Viscum ramosissimum Well, Viscum capitallatum Sm, Viscum trilobatum Talbot were found to be highly toxic. At non-toxic dosage, they could reduce the transplanted tumors in animals (Kuttan et al, 2017). In the present study, we have studied the immunostimulating activity of these Viscum species and special emphasis has been given to the non-toxic plant, V. angulatum.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Original article Immunopotentiating activity of hemiparasitic plants of family Viscaceae with special reference to Viscum angulatum Heyne ex DC.

Kuttan¹,

Kuttan²

2019

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The present study was carried out to evaluate immunopotentiating activity of hemiparasitic sub-herbs plants belongs to Viscaceae family. Aqueous extracts of selected six Viscum plants such as, Viscum orientale Wild (8 mg/ kg body weight), Viscum nepalense Spiens (8 mg/kg body weight), Viscum ramosissimum Well (8 mg/kg body weight), Viscum angulatum Heyneex DC (16 mg/kg body weight), Viscum capitallatum Sm (8 mg/kg body weight) and Viscum trilobatum Talbot (8 mg/kg body weight) were injected intraperitoneally for 5 consecutive days to Balb/c mice and to determine the haematological parameters, body weight, lymphoid organ weight, bone marrow cellularity and α-esterase positive cells and compared with controls treated with plain water. Non-toxic V. angulatum extract (16 mg/kg) was administered to Balb/c mice (5 doses) and its effect on antibody titre, antibody forming cells, lymphoid cell proliferation, cytotoxic T lymphocyte generation and interferon γ (IFN-γ) and interleukin 2 (IL-2) levels were determined. Results indicated that there was a significant increase in white blood cells (WBC), lymphoid organ weight, bone marrow cellularity and α-esterase positive cells after intraperitoneal administration. Administration of V. angulatum extract increased antibody titre, antibody forming cells, proliferation of lymphoid cells, stimulated cytotoxic T lymphocyte as well as increased levels of IL-2 and IFN-γ. Results indicated a stimulation of immunological parameters in mice after intraperitoneal injection of aqueous extracts of plants belonging to the family Viscaceae.

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Potential Anti-Tumor and Anti-Inflammatory Activity of Six Mistletoe Plants in the Family Viscaceae Present in Western Ghats, India

Cited by 5 publications

References 28 publications

In Vitro Cytotoxicity Activity of Methanolic Leaf and Fruit Extract of Zanthoxylum Ovalifolium Wight Against Dalton’s Lymphoma Ascites and Ehrlich Ascites Carcinoma Cancer Cell Lines

In Vitro Cytotoxicity Activity of Methanolic Leaf and Fruit Extract of Zanthoxylum Ovalifolium Wight Against Dalton’s Lymphoma Ascites and Ehrlich Ascites Carcinoma Cancer Cell Lines

Anti-Inflammatory Properties of Zanthoxylum Ovalifolium Weight Methanolic Extract on Albino Wistar Rats

Original article Immunopotentiating activity of hemiparasitic plants of family Viscaceae with special reference to Viscum angulatum Heyne ex DC.

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