Potential Antioxidant and Anti-Inflammatory Function of Gynura procumbens Polyphenols Ligand

Kim, Hun Hwan; Ha, Sang Eun; Vetrivel, Preethi; Bhosale, Pritam Bhagwan; Kim, Sang Woo

doi:10.3390/ijms22168716

Cited by 11 publications

(8 citation statements)

References 46 publications

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“…In previous studies, three compounds with antioxidant and anti-inflammatory potential were selected [ 23 ], and kynurenic acid (KYNA), which has the highest molecular binding affinity through molecular docking analysis, was used for future analysis. KYNA is a physiologically active ingredient found in various herbs [ 24 ], and is known to induce apoptosis through cell cycle regulation and signal transduction pathways in colon cancer cells [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

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Cellular Regulation of Kynurenic Acid-Induced Cell Apoptosis Pathways in AGS Cells

Kim

Jeong

et al. 2022

IJMS

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Kynurenic acid was included in the three compounds (caffeic acid, chlorogenic acid, and kynurenic acid) that showed high antioxidant and anti-inflammatory potential among the phenolic compounds contained in Gynura procumbens. In this study, the mechanism of cancer cell death induced by kynurenic acid (KYNA), which has the highest molecular binding affinity, in the gastric cancer cell line AGS was confirmed in molecular docking analysis. KYNA showed the most cancer cell death effect on AGS cells among several gastric cancer cell lines (MKN, AGS, and SNU). AGS cells were used for later experiments, and KYNA concentrations of 0, 150, 200, and 250 µM were used. KYNA inhibited cell migration and proliferation in AGS cells in a concentration-dependent manner. G2/M phase cell cycle arrest and reduction of related proteins (Cdc25C, CDK1 and CyclinB1) were confirmed in KYNA-treated AGS cells. Apoptosis of KYNA-treated AGS cells was confirmed by Annexin V/propidium iodide (PI) staining flow cytometry analysis. As a result of morphological chromatin condensation through DAPI (4′,6-diamidino-2-phenylindole), intense blue fluorescence was confirmed. The mechanism of apoptosis induction of KYNA-treated AGS cells was confirmed by western blotting. In the extrinsic pathway, apoptosis induction markers FasL, Fas, and Caspase-3 and -8 were increased in a concentration-dependent manner upon KYNA treatment. In the intrinsic pathway, the expression of anti-apoptotic factors PI3K, AKT, and Bcl-xL was down-regulated, and the expression of apoptosis-inducing factors BAD, Bak, Bax, Cytochrom C, and Caspase-9 was up-regulated. Therefore, in the present study, we strongly imply that KYNA induces apoptosis in AGS gastric cancer cells. This suggests that KYNA, a natural compound, could be the basis for drug for the treatment of gastric cancer.

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Section: Discussionmentioning

confidence: 99%

“…In our previous study, we selected three compounds with antioxidant and anti-inflammatory potentials [ 23 ]. The binding affinity of the three compounds with the tumor-inducing factor (TOP1) was confirmed.…”

Section: Methodsmentioning

confidence: 99%

Cellular Regulation of Kynurenic Acid-Induced Cell Apoptosis Pathways in AGS Cells

Kim

Jeong

et al. 2022

IJMS

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“…As a result, our work is the first to identify LCDE substances and screen DPPH ligand using UF-LC–MS analysis, based on the idea that unique organic chemicals and enzymes bind to target receptors 25 . These findings point to a way for screening complex compounds simultaneously in the early phases of drug development, as well as a strong tool for identifying possible oxidative stress and inflammatory ligands from a variety of medicinal plants.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant effects of phenolic compounds in through the distillation of Lonicera japonica & Chenpi extract and anti-inflammation on skin keratinocyte

Kim,

Jeong,

Park

et al. 2023

Sci Rep

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The phenolic compounds in Lonicera japonica & Chenpi distillation extract (LCDE) were thoroughly examined for their antioxidant and anti-inflammatory properties. Phenolic compounds in LCDE were analyzed for five peaks using high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS) and determined. Five phenolic compounds were identified from the samples and MS data. Ultrafiltration with LC analysis was used to investigate the ability of bioactive compounds to target DPPH. As a result, it was confirmed that the major compounds exhibited a high binding affinity to DPPH and could be regarded as antioxidant-active compounds. In addition, the anti-inflammatory effect of LCDE was confirmed in vitro, and signal inhibition of anti-inflammation cytokines, MAPK and NF-kB pathways was confirmed. Finally, Molecular docking analysis supplements the anti-inflammatory effect through the binding affinity of selected compounds and inflammatory factors. In conclusion, the phenolic compounds of the LCDE were identified and potential active compounds for antioxidant and anti-inflammatory activities were identified. Additionally, this study will be utilized to provide basic information for the application of LCDE in the pharmaceutical and pharmaceutical cosmetics industries along with information on efficient screening techniques for other medicinal plants.

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“…In an examination of antioxidant polyphenols, the complex technology of organoids as a new anti-cancer strategy has displayed unique advantages. Polyphenols that largely exist in plants serve as reactive oxygen species scavengers with prominent antioxidant activity, which exert the inhibitory effects on inflammation by manipulating the inflammation-related signaling pathways and suppressing the release of inflammatory mediators ( 48 , 49 ). Not only contributing to treating non-neoplastic diseases, such as natural inducers from saffron targeting Nrf2/vitagene pathway to suppress oxidative stress and neuroinflammation and consequently inhibiting cognitive dysfunction ( 50 ), polyphenols could also play their roles in preventing and treating tumors by regulating cell apoptosis, autophagy, cell-cycle progression, inflammation, invasion, and metastasis through the activation of tumor suppressor genes and the inhibition of oncogenes ( 51 , 52 ).…”

Section: Discussionmentioning

confidence: 99%

Progress of research on tumor organoids: A bibliometric analysis of relevant publications from 2011 to 2021

et al. 2023

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BackgroundResearch on tumor organoids has developed rapidly over the past 20 years, but a systematic analysis of current research trends is lacking. Researchers in the field need relevant references and knowledge of current research hot spots. Bibliometric analysis and visualization is a systematic method of acquiring an in-depth understanding of the status of research on tumor organoids.MethodsCiteSpace, VOSviewer and the Bibliometric Online Analysis Platform from the Web of Science Core Collection were used to analyze and predict publishing trends and research hot spots worldwide in the field of tumor organoids.ResultsA total of 3,666 publications on tumor organoids were retrieved, and 2,939 eligible articles were included in the final analysis. The number of publications has grown significantly, with the United States of America as the leading country for research on tumor organoids. Among journals, Cancers published the largest number of articles. Harvard Medical School published the highest number of articles among all institutions. The Chinese Academy of Sciences was ranked highest among all contributing institutions on the importance of their publications. A trend in multi-disciplinary collaboration was observed in studies on tumor organoids. Keywords indicated that the current research largely concentrated on optimizing the construction of organoid models to use for medication development and screening in the clinical setting, and to provide patients with individualized treatment for gastric cancer and colorectal cancer, which are newly emerging research hotspots. Gastric and colorectal cancers were the top two tumors that have received increasing attention and have become the focal points of recent studies.ConclusionThis study analyzed 2,939 publications covering the topic of tumor organoids. Although optimizing the construction of organoid models has always been a hot topic in this field, the application of tumor organoids to the development of medications and screenings will foster individualized treatment for patients, which is another emerging hot spot in this field of research.

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Potential Antioxidant and Anti-Inflammatory Function of Gynura procumbens Polyphenols Ligand

Cited by 11 publications

References 46 publications

Cellular Regulation of Kynurenic Acid-Induced Cell Apoptosis Pathways in AGS Cells

Cellular Regulation of Kynurenic Acid-Induced Cell Apoptosis Pathways in AGS Cells

Antioxidant effects of phenolic compounds in through the distillation of Lonicera japonica & Chenpi extract and anti-inflammation on skin keratinocyte

Progress of research on tumor organoids: A bibliometric analysis of relevant publications from 2011 to 2021

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