Potential application of an injectable hydrogel scaffold loaded with mesenchymal stem cells for treating traumatic brain injury

Zhang, Kun; Shi, Zhenqing; Zhou, Jian‐Kang; Xing, Qu; Ma, Shanshan; Li, Qinghua; Zhang, Yanting; Yao, M.; Wang, Xiaofeng; Li, Qian; Li, Jingan; Guan, Fangxia

doi:10.1039/c7tb03213g

Cited by 94 publications

(77 citation statements)

References 41 publications

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“…A temperature scan with a heating rate of 2/min from 17°C to 90°C was performed, the analysis frequency was 1 rad/s, and the change curves of the G ′, G ″, phase angle, and complex viscosity were recorded; the intersection of G ′ and G ″ is defined as gelation temperatures ( T gel , Chenite, 2000). The morphology of the gelling hydrogel was observed by scanning electron microscopy (QUANTA200, Holland FEI, The Netherlands) after freeze‐drying and gold spraying (Zhang, Shi, et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Nowadays, tissue engineering is widely recognized as a hot topic in the field of biomedical science, and it is playing more and more crucial roles for organ/tissue repair and replacement (Caccavo, Cascone, Lamberti, & Barba, ). Current technologies have achieved the aims of implanting the biomaterials loaded with the massive in vitro cultured cells into the human body in order to restore, replace, maintain, or improve the function of tissues or organs (Zhang et al, ). However, only a few engineered tissues or organs have clinical application.…”

Section: Introductionmentioning

confidence: 99%

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An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

Zhao

et al. 2019

J Biomedical Materials Res

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Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration. A biomedical application requires the selective release of multiple factors which will guide the synergy of the cells. Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release. Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization. In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

Zhao

et al. 2019

J Biomedical Materials Res

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“…In recent years, hydrogels have been widely used in tissue engineering and nerve regeneration due to their good biocompatibility, water retention and drug‐delivery properties . Ortuno‐Lizaran et al fabricated biodegradable hyaluronic acid hydrogel conduit by chemical cross‐linking, and found that Schwann cells could grow well in the conduit lumen.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, hydrogels have been widely used in tissue engineering and nerve regeneration due to their good biocompatibility, water retention and drug-delivery properties. [18][19][20][21][22][23][24] Ortuno-Lizaran et al 25 fabricated biodegradable hyaluronic acid hydrogel conduit by chemical cross-linking, and found that Schwann cells could grow well in the conduit lumen. Gonzalez-Perez et al 26 prepared collagen hydrogel-filled chitosan conduits containing extracellular matrix, the conduit could promote the repair of peripheral nerve injury to a certain extent, which may be used as a potential candidate in the repair of peripheral nerve injury.…”

Section: Introductionmentioning

confidence: 99%

PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury

Chen

Zhao

Yan³

et al. 2019

J Biomedical Materials Res

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The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research. The aim of this study was to construct a bioactive composite hydrogel conduit as a three‐dimensional scaffold for the repair of rat sciatic nerve defects. The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self‐made mold with an inner diameter of 2 mm and an outer diameter of 6 mm. Then the conduit was further modified with YIGSR peptide. The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration. The results of diameter analysis indicated that the wall of hydrogel conduit was uniform. The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties. Further on, hematoxylin–eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve. Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1273–1283, 2019.

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“…To investigate the modified layers' biomechanical properties, the stress values of each sample were detected by a microcomputer controlled electronic universal testing machine (WDW_200, Shanghai Hualong testing instrument Limited by Share Ltd., Shanghai, China). The weight loss of all the samples was also measured to evaluate the layers' biodegradability properties [20].…”

Section: Characterization Of Pda/pei/5-fu Layersmentioning

confidence: 99%

Surface Modification of Esophageal Stent Materials by a Drug-Eluting Layer for Better Anti-Restenosis Function

Bai

Zhang

et al. 2018

Coatings

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Abstract:It is generally accepted that stent implantation is the mainstream therapy in clinics for esophageal cancer in the later period. However, the restenosis caused by tumor cells, epithelial cells, and fibroblasts seriously interferes with the stent medical application and limits its long-term services. To address this conundrum, a series of drug-eluting stents were invented and verified to be feasible in the early stage after implantation, but the limited drug loading and good cell compatibility of the stent materials may lead to more serious restenosis and further endanger the patient's life. In previous work, we modified the esophageal stent material 317L stainless steel (317L SS) surface with a poly-dopamine/poly-ethylenimine layer (PDA/PEI), which had strong anti-tumor functions. In this contribution, we employed a usual drug in clinic, 5-fluorouracil (5-Fu), with series of density onto the PDA/PEI modified 317L SS to investigate the influence of 5-Fu immobilization on the anti-restenosis function. The surface characterization including 5-Fu quantity, atomic force microscopy (AFM). Water contact angle measurement indicated successful preparation of the PDA/PEI/5-Fu layers. The spectrophotometric characterization revealed that the immobilized 5-Fu rapidly released over 24 h. However, the Eca109, Het-1A, and L929 cells culture results suggested that the released 5-Fu made a significant contribution to improving the apoptosis and necrosis of these pathological cells, and the PDA/PEI/5-Fu layers maintain the consistent anti-restenosis function on their surfaces with the PDA/PEI layer after 24 h. All the results demonstrated the PDA/PEI/5-Fu layers' excellent ability to suppress esophageal tumor cells, epithelial cells, and fibroblasts, suggesting a potential application on the surface modification of esophageal stents for better anti-restenosis function.

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Potential application of an injectable hydrogel scaffold loaded with mesenchymal stem cells for treating traumatic brain injury

Abstract: In this contribution, we developed an injectable hydrogel composed of sodium alginate and hyaluronic acid that acts as a tissue scaffold to create a more optimal microenvironment for the stem cells for potential application of traumatic brain injury implantation.

Cited by 94 publications

References 41 publications

An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury

Surface Modification of Esophageal Stent Materials by a Drug-Eluting Layer for Better Anti-Restenosis Function

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