Potential Application of Bacillus subtilis Lipopeptides in Toothpaste Formulation

Abstract

Toothpaste is a gel dentifrice used with a toothbrush as an accessory to clean, keep and promotes oral hygiene. Bio toothpaste is the toothpaste that contains biologically active ingredient. In the present work Bacillus subtilis was used as a source for the production of bio surfactant (lipopeptides) or emulsifiers. After 2 days of production period bio surfactant was extracted using acid alkali precipitation method. And finally obtained powdered bio surfactant was used along with the other chemical ingredients for the formation of bio toothpaste. The physicochemical properties were analyzed considering several tests mainly spreading ability, water activity, and pH, foaming and cleaning tests. The evaluation of the antimicrobial activity of the formulated dentifrice was carried out against different bacteria.