Potential binding of borate ions to mono- and oligonucleotides: a capillary electrophoresis investigation

D’Acunto, Maria Rosa; Gelfi, Cecilia; Stoyanov, Alexandre; Andreev, Victor P.; Righetti, Pier Giorgio

doi:10.1016/s0021-9673(02)01500-5

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…Capillary zone electrophoresis is the most widely used mode of CE under native conditions and it is also the first fully automated electrophoresis method (Li et al. 2001; D'Acunto et al. 2002; Ling et al.…”

Section: Discussionmentioning

confidence: 99%

A comparative study on the production of exopolysaccharides between two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis in submerged mycelial cultures

Kim

Yun

2005

J Appl Microbiol

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Aims: The present study comparatively investigates the optimal culture conditions for the production of exopolysaccharides (EPS) and cordycepin during submerged mycelial culture of two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis. Methods and Results: Fermentations were performed in flasks and in 5-l stirred-tank fermenters. In the case of C. militaris, the highest mycelial biomass (22AE9 g l )1 ) and EPS production (5 g l )1 ) were achieved in a medium of 40 g l )1 sucrose, 5 g l )1 corn steep powder at 30°C, and an initial pH 8AE0. The optimum culture conditions for C. sinensis was shown to be (in g l )1 ) 20 sucrose, 25 corn steep powder, 0AE78 CaCl 2 , 1AE73 MgSO 4 AE7H 2 O at 20°C, and an initial pH 4AE0, where the maximum mycelial biomass and EPS were 20AE9 and 4AE1 g l )1 respectively. Cordycepin, another bioactive metabolite, was excreted at low levels during the early fermentation period (maximum 38AE8 mg l )1 in C. militaris; 18AE2 mg l )1 in C. sinensis). Conclusions:The two fungi showed different nutritional and environmental requirements in their submerged cultures. Overall, the concentrations of mycelial biomass, EPS and cordycepin achieved in submerged culture of C. militaris were higher than those of C. sinensis. Significance and Impact of the Study: C. militaris and C. sinensis are representative insect-born fungi which have been longstanding and widely used as traditional medicines in eastern Asia. Comparative studies between two fungi are currently not available and this is the first report on the optimum medium composition for submerged culture of C. sinensis.

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Section: Discussionmentioning

confidence: 99%

A comparative study on the production of exopolysaccharides between two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis in submerged mycelial cultures

Kim

Yun

2005

J Appl Microbiol

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“…So, using negative-end detection mode, AMPs take a longer time to reach the detection window than that in neutrally aqueous BGE. To reduce the migration time of AMP and improve the resolutions of AMPs, boric acid was usually employed, because borate can interact with the vicinal diols of nucleotides to form anionic complexes [34][35][36][37][38] in basic pH condition. In addition, Tris, an organic cation, was usually used as a buffering group in CE [32].…”

Section: Choice Of Buffer System In Nacementioning

confidence: 99%

Development of a NACE method for simultaneous measurement of three adenosine monophosphate isomers in biomimicking prebiotic synthesis without sample pretreatment

et al. 2006

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A practical NACE method was developed for simultaneous determination of three adenosine monophosphate (AMP) isomers. Separation of three AMP isomers was achieved using 200 mM Tris/H(3)BO(3) in acetontrile/water (2:1 v/v) at pH* 10.0 as the running buffer and +25 kV as the applied voltage over a bare fused-silica capillary of 50 microm id x 375 microm od x 54.5 cm (46 cm to the detector window). At 260 nm, the calibration curves were linear in the range of 1-100 microg/mL. The detection limits were less than 0.70 microg/mL. The recovery ranged from 94.5 to 106.4%. The intraday RSDs of the migration times were between 2.1 and 3.0%. The developed NACE method has been successfully applied for the determination of three AMP isomers in the real samples of biomimicking prebiotic synthesis reaction between N-(O,O-diisopropyl) phosphoryl amino acid and adenosine.

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“…The results indicated that borate could form a complex with the glucose in the open-chain form but, under conditions of very high borate concentrations, the pyranose form also complexes with borate. Recently, D'Acunto et al [24] also systematically studied the potential binding of borate ions to mono-and oligonucleotides.…”

Section: Interaction Of Nucleotides With Boratementioning

confidence: 99%

An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

Lin

Liu

2003

Electrophoresis

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The electrochromatographic separations of 2'-, 3'- and 5'-monophosphates of adenosine, guanosine, cytidine, and uridine were carried out with an open-tubular capillary column which was wall-coated with a highly selective reagent, 28-membered macrocyclic polyamine, 4, 8, 12, 18, 22, 26-hexaaza-1,15-dioxacyclooctaeicosane ([28]ane-N6O2). The effects of pH, composition and concentration of background electrolyte (BGE), applied voltage, column length, and the additive of the BGE, such as metal ions, borate, beta-cyclodextrin and organic solvent on the separation of these monophosphorylated nucleotide isomers were investigated. The results suggested that the interactions between analytes and the bonded groups on the wall predominantly comprise anion coordination and anion exchange in addition to the electrophoresis. A well-resolved electrochromatogram was obtained with the capillary column of 100 cm (75 cm effective length) x 75 microm inside diameter (ID), citrate buffer (20 mM, pH 3.99), applied voltage of -22 kV and detection at 254 nm. Column efficiency was found with the average theoretical plate numbers of 119,500/m and a low detection limit of 0.01 microM level could be achieved for the separation of these isomers.

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Potential binding of borate ions to mono- and oligonucleotides: a capillary electrophoresis investigation

Cited by 4 publications

References 20 publications

A comparative study on the production of exopolysaccharides between two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis in submerged mycelial cultures

A comparative study on the production of exopolysaccharides between two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis in submerged mycelial cultures

Development of a NACE method for simultaneous measurement of three adenosine monophosphate isomers in biomimicking prebiotic synthesis without sample pretreatment

An insight into the phenomena involved in a multiple‐function stationary phase for the capillary electrochromatographic separation of 2'‐, 3'‐, and 5'‐monophosphorylated nucleoside isomers

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