Potential Changes in Watermelon (Citrullus lannatus) Ploidy Treated By Colchicine

Handayani, Rd. Selvy; Yusuf, Muhamad Nurdin; Akmal, Ajmir

doi:10.33089/jthort.v1i1.6

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…Souza et al (2001), when treating seeds of three watermelon lineages with 0.2% colchicine solution for 24 h, deemed 2.7% of the plants tetraploids by morphological analysis and chloroplast count, and when these plants were crossed with diploid lines, they generated triploid hybrids. Handayani, Yusuf and Akmal (2018) also obtained good results in changing the chromosome number of watermelon plants (F 1 hybrid) by applying 0.2% colchicine for 48 h.…”

Section: Resultsmentioning

confidence: 90%

INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii)

et al. 2022

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Triploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew–resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant.

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Section: Resultsmentioning

confidence: 90%

INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii)

et al. 2022

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“…Polyploidization is generally recognized as an effective strategy to improve organ size. Handayani et al, (2018), investigated that the larger cells produce larger parts of the plant such as leaves, flowers, fruits, and plants. Whereas the increase in leaf area leads to increase the biological processes such as photosynthesis which in turn leads to increase the growth of plant (Beest et al, 2012 andLi et al, 2014).…”

Section: Red Indicates the Highest Mean Of Seed Germination And Seedlmentioning

confidence: 99%

Influence of Colchicine Treatment on Morphological, Physiological and Anatomical Cercis siliquastrum L. Seedlings Growth تأثیر معاملة الکولشیسین على النمووالصفات المظهریة و الفسیولوجبة و التشریحیة لشتلات الأرغوان

سليمان¹,

أسکندر²

2019

Journal of Plant Production

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The present study was conducted to assess the effect of polyploidy induction on different properties of Cercis siliquastrum plant. Experiment was conducted in RCBD with 15 treatments and 3 replications. The seeds were treated with 0.0, 0.5, 1.0, 1.5 and 2.0 % colchicine solution at room temperature for various exposure times 12, 24 and 48 h. Observations on morphological variations were recorded on each plant in each treatment. Various in seed germination, morphological and growth characteristics observed due to effects of interactions between colchicine concentration and soaked period. By increasing of the colchicine concentration the seed germination rate decreased significantly from 80% to 50%, the effects on seed germinate were most evident at the higher colchicine concentrations (1.5 to 2.0 %). Longer stem and root, thicker stem and root diameter, higher number of leaves, branch and roots per transplant, were width area, thick and greener leaves, variable anatomy wood stem were achieved and development as compared to control plants. The length and width of stomata (21.6 and 10.3µm) respectively were significantly increased and induced in 0.1% of colchicine for 48h, whereas stomata density was decreased to 68 per area in 0.2% of colchicine at 48h. in contrast with initial diploid plants. However 1.5 % and 0.2% concentrations of colchicine at different soaked time were more effective for producing variation in plants. These polyploids plants may be helpful for further development and improvement of ornamental tree.

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Potential Changes in Watermelon (Citrullus lannatus) Ploidy Treated By Colchicine

Cited by 2 publications

References 5 publications

INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii)

INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii)

Influence of Colchicine Treatment on Morphological, Physiological and Anatomical Cercis siliquastrum L. Seedlings Growth تأثیر معاملة الکولشیسین على النمووالصفات المظهریة و الفسیولوجبة و التشریحیة لشتلات الأرغوان

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