Potential cow milk xanthine oxidase inhibitory and antioxidant activity of selected phenolic acid derivatives

Abstract

Xanthine oxidase (XO) found in all mammals and excess activity leads to urolithiasis. The cow milk XO was purified to 305-fold with a specific activity of 8.76 EU/mg and overall yield of 87% by using DEAE-Sepharose chromatography. The phenolics showed potent XO inhibitory effect with K i , P1

Antihyperuricemic effect of dietary polyphenol sinapic acid commonly present in various edible food plants

“…Cos et al (1998) 2 scavenging nor XOD inhibition activity, or type VIadditional pro-oxidant activity but no XOD inhibition. Similar results were also observed by Nile et al (2018), who reported that phenolic acids potently inhibit the XOD enzyme and possess a strong antioxidant activity. Similar results were also observed by Nile et al (2018), who reported that phenolic acids potently inhibit the XOD enzyme and possess a strong antioxidant activity.…”

“…Previously, various authors reported that hydroxycinnamic acid, flavonoids, and other bioactive compounds potently inhibit the XOD enzyme (Nile et al, 2018;Nguyen et al, 2006;Pathak, Gupta, Shukla, & Baunthiyal, 2018;Qu et al, 2017;Zhang et al, 2016). The A similar inhibitory mode has been observed in various bioactive compounds Gawlik-Dziki et al, 2017;Lin et al, 2015;Pathak et al, 2018;Zhang et al, 2016).…”

“…It has been reported that XOD inhibitors act as antioxidants to decrease ROS and prevent oxidative stress (Kumar et al, 2018;Lin et al, 2015;Nile et al, 2018). Herein, SA was shown to inhibit the XOD enzyme and to have moderate O −• 2 scavenging activity and a strong DPPH radical scavenging activity.…”

“…Previously, various authors reported that hydroxycinnamic acid, flavonoids, and other bioactive compounds potently inhibit the XOD enzyme (Nile et al, 2018;Nguyen et al, 2006;Pathak, Gupta, Shukla, & Baunthiyal, 2018;Qu et al, 2017;Zhang et al, 2016). The methylation and esterification of the OH groups of SA play an important role in XOD inhibition.…”

“…Previously, Nile, Keum, Nile, Kwon, and Kim (2018) and Lin et al (2018) reported that SA potently inhibited the XOD enzyme.…”