Potential exploitation of Bacillus flexus biofilm against the cowpea weevil, Callosobruchus maculatus (F.) (Coleoptera: Bruchidae)

Reda, Fayiz M.; Hassanein, Wesam A.; Moabed, Shahera; El-Shafiey, Samah N.

doi:10.1186/s41938-020-00222-3

Cited by 6 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B. acidophilus was reported as cariogenic agent by Tucker [56]. Biofilm formation by Bacillus species was reported previously [57]. Bacillus subtilis from the oral cavity was reported by Yamane et al [58].…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

et al. 2022

View full text Add to dashboard Cite

Among oral diseases, dental caries is one of the most frequent to affect human health. The current research work aimed to ascertain the antibacterial, anti-biofilm, and antioxidative potential of Piper betle leaf extract against bacteria isolated from dental caries. Analysis for the presence of phytochemical compounds revealed compounds, such as tannins, steroids, phenolic compounds, and alkaloids, which were also confirmed by TLC and FTIR. GC-MS analysis elucidated the presence of 20 phytocompounds, among which were some well-reported bioactive compounds. The chloroform extract of P. betle demonstrated good antibacterial activity (7 mm) and minimum inhibitory concentration (MIC) (100 mg mL−1) against Bacillus gaemokensis MW067143, which was the frequent biofilm producer among isolated bacterial strains. Fractions of the extract were isolated through column chromatography, after which the antibacterial activity was again evaluated. Spirost-8-en-11-one,3-hydroxy(3β,5α,14β,20β,22β,25R), an oxosteroid in nature, was observed to exhibit remarkable antibacterial potential (12 mm) against B. gaemokensis. Bacterial cells treated with P. betle extract had elevated SOD, APOX, POX, and GR activity, while its proteolytic activity against whole bacterial proteins was pronounced with the suppression of several proteins (50, 40, 15, and 10 kDa) in SDS-PAGE. Bacterial cells treated with P. betle extract demonstrated decreased growth, while the extract was also observed to exhibit inhibition of biofilm formation (70.11%) and demolition of established B. gaemokensis biofilms (57.98%). SEM analysis revealed significant changes to bacterial morphology post treatment with P. betle, with cellular disintegration being prominent. In silico network pharmacology analysis elucidated proteins like ESR1 and IL6 to be majorly involved in biological pathways of dental caries, which also interact with the protective ability of P. betle. Gene Ontology (GO) terms and KEGG pathways were also screened using enrichment analysis. Molecular docking demonstrated the highest binding affinity of Spirost-8-en-11-one,3-hydroxy-,(3β,5α,14β,20β,22β,25R) with bacterial proteins FabI (−12 kcal/mol), MurB (−17.1 kcal/mol), and FtsZ (−14.9 kcal/mol). Therefore, it is suggested that P. betle can serve a potentially therapeutic role and could be used in the preparation of herbal formulations for managing bacterial flora.

show abstract

Section: Discussionmentioning

confidence: 89%

“…It was first reported by Jung et al [59] from tidal flat sediment of the Yellow Sea. Similarly, Bacillus pumilis and Bacillus flexus are the environmental microorganisms, among which the latter is known for its biofilm production [57].…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In contrast, bio-concrete samples demonstrated crystalline surfaces where distinct CaCO3 crystals may recognize (Krishnapriya et al, 2015;Siddique et al, 2016;Alshalif et al, 2019). Reda et al, (2020) were reported that Bacillus flexus is the most potent one amongst the bacterial isolates that can form biofilm. Also, Rajitha et al, (2020) was noted that Bacillus haynesii produce acid-soluble extracellular biopolymer.…”

Section: Visual Observation Of Self-healingmentioning

confidence: 98%

Sustainable Repairing and Improvement of Concrete Properties Using Bacterial Consortium Isolated From Egypt

Mahmoud¹,

Kalaba

El-Sherbiny

et al. 2021

Preprint

View full text Add to dashboard Cite

The small cracks in concrete constructions are inevitable due to deterioration during their service life throughout different load combination factors. In this study, we aimed to isolate, identify, and construct a bacterial consortium able to heal small cracks of concrete and enhance the different properties of concrete. Six isolates of bacillus, endospore-forming bacteria were isolated. There are only three isolates out of the six coded as NW-1, MK and NW-9 were showed the ability to produce urease enzyme and able to grow at 60°C with optimum growth at a temperature of 40°C. These isolates were survived in high pH, where isolate NW-1 was tolerated pH up to 11 with optimum growth at 10 while the isolates NW-9 and MK showed growth at pH 12 with an ideal growth at 10. CaCO3 production was observed by the three bacterial isolates whether in pure or mixed cultures (bacterial consortium) but the consortium consisting of MK and NW-9 was significantly the highest in productivity among them. Therefore, these two isolates were identified using 16s as Bacillus flexus MK-FYT-3 and Bacillus haynesii MK-NW-9 and deposited to GenBank under accession numbers MN965692 and MN965693 respectively. The effect of bacteria on some properties of concrete was studied, and the results showed that the compressive and tensile strengths of bio-concrete specimens were significantly increased by 31.29, 29 % after 7 days and 36.3, 39 % after 28 days of curing compared to control specimens. The results of permeability indicated that the bio-concrete specimens significantly showed less permeability than the control specimens by 21.1, 23.1% after 7 and 28 of curing, respectively. To determine the concrete density, Ultrasonic Pulse Velocity (UPV) test was performed, and the bio-concrete specimens gave higher values than control specimens by 26 and 20% after curing for 7 and 28 days, respectively. Also, surface healing of concrete was observed visually, the bio-concrete showed white precipitates around and inside the cracks after 7 days, which led to almost complete sealing of concrete after 28 days of curing, while the control samples were showed only very slight deposits on the surface and away from the cracks. The micro-analysis of concrete samples using SEM and XRD were done. It was found that the bio-concrete specimens showed crystalline precipitate with different shapes under SEM, while no such deposits appeared in the control specimens. On the other hand, the XRD profile was explained the characteristic peaks of calcium carbonate in both the bio-concrete and the control specimens, but the peak intensity was higher in the bio-concrete than the control specimens. This reflects the effectiveness of bacterial consortium in repairing and preventing the concrete cracks from spreading in addition to improving the various properties of concrete leading to increasing its life and sustainability.

show abstract

“…On the other hand, samples of bio-concrete showed crystalline surfaces with distinct CaCO3. [56, -58].According to [59] of the bacterial isolates that can create biofilm, Bacillus flexus is the most effective. It was also reported by [60] that Bacillus haynesii produces extracellular biopolymer that is soluble in acid.…”

Section: Visual Vp-sem-edsmentioning

confidence: 99%

Manufacture and Study of Self-Healing Properties in Concrete With Bacillus Haynessii (Rakan1508) Bacteria Isolated From Farafra Oasis, New Vally Governorate, Egypt

Abbas,

Nooman,

Ismael

et al. 2024

Journal of Al-Azhar University Engineering Sector

View full text Add to dashboard Cite

Concrete failure can be attributed to various factors, including cracks that allow chemical solutions to seep through this may have a substantial effect on Concrete Structures' Mechanical, Physical, and Durability Aspects. The idea that bones and tissues can repair themselves has been exploited, so we needed to make concrete that can self-heal cracks. The ability of a material to identify and mend internal damage without help from outside sources is known as self-healing. Our goal of this work was to identify, isolate, and create a bacterial consortia that could repair tiny concrete cracks and improve the concrete many qualities. Bacillus bacteria that generate endospores were discovered in four different isolates. Of the four isolates, only one was able to grow at 50°C and produce the urease enzyme; 40°C is the optimal temperature for growth. It also demonstrated growth potential at a pH of 12 with optimal growth at 10. Therefore, this isolate was identified using 16s as Bacillus haynesii RAKAN1508 and is stored in Gen-Bank with accession number OR642761. The progression of the healing ratio and the fracture profile were evaluated using both the scanning electron microscope with variable pressureCT scans using X-rays (also known as X-ray mCT). Additionally, this Studies were conducted to determine how bacteria affected properties concrete qualities. VP-SEM results showed that microbial precipitation fully healed a 0.4 mm crack mouth width, which XRD subsequently confirmed to be calcite and vaterite. Therefore, compared to control specimensthe ,bio-concrete specimens' compressive and tensile strengths increased dramatically by 36.3 and 44% following 28 days of curing. Per the sorpativity test results, which showed that after 28 days of healing, In contrast to the control samples, the bio-concrete specimens showed a substantial 23.1% decrease in permeability.The long-term repair of cracks in the concrete skin may be achieved with bacteria-based concrete self-healing.

show abstract

Potential exploitation of Bacillus flexus biofilm against the cowpea weevil, Callosobruchus maculatus (F.) (Coleoptera: Bruchidae)

Cited by 6 publications

References 24 publications

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

Investigation of the Antibacterial, Anti-Biofilm, and Antioxidative Effect of Piper betle Leaf Extract against Bacillus gaemokensis MW067143 Isolated from Dental Caries, an In Vitro-In Silico Approach

Sustainable Repairing and Improvement of Concrete Properties Using Bacterial Consortium Isolated From Egypt

Manufacture and Study of Self-Healing Properties in Concrete With Bacillus Haynessii (Rakan1508) Bacteria Isolated From Farafra Oasis, New Vally Governorate, Egypt

Contact Info

Product

Resources

About