Potential involvement of dendritic cells in delayed-type hypersensitivity reactions to β-lactams

Mass Spectrometric Characterization of Circulating and Functional Antigens Derived from Piperacillin in Patients with Cystic Fibrosis

“…Ag (43,44), was not seen in piperacillin-hypersensitive patients, and this may relate to the absence of eosinophilia as a clinical feature. The detection of IL-1b, TNF-a, and IL-6 in culture supernatant containing piperacillin-stimulated PBMCs from hypersensitive patients and tolerant controls (albeit to a lesser extent) are suggestive of a dendritic cell response against piperacillin, as has recently been described with amoxicillin (45,46). In support of this argument, piperacillin-specific T cell clones do not secrete IL-1b (Sabah El-Ghaiesh, B. Kevin Park, and Dean J. Naisbitt, unpublished observations).…”

The expression of genes involved in jejunal lipogenesis and lipoprotein synthesis is altered in morbidly obese subjects with insulin resistance

“…Cells were kept on ice during 30 min and then were incubated with a rat monoclonal IgG antihuman A33-Alexa Fluor 488 (Clone # 402104) (R&D Systems, Minneapolis, MN), 15 and a monoclonal IgG antihuman-CD45-APC, a marker of all leukocyte groups (BD Pharmingen, San Jose, CA). Appropriate isotype controls were included (IgG2a-Alexa Fluor 488 (R&D Systems) and IgG1-APC, (BD Pharmingen)) as described 16 and analyzed by flow cytometry in a FACS CANTO II (Becton-Dikinson Biosciences, San Jose, CA, USA). Data were analyzed using FlowJo Software.…”

Drug-Induced Anaphylaxis

“…Other techniques, such as lymphocyte-stimulation-based tests, originally developed for T cell-mediated reactions [90,91], have also been applied to IgE-dependent reactions [91,92].…”