Potential Link between Ossification of Nuchal Ligament and the Risk of Cervical Ossification of Posterior Longitudinal Ligament: Evidence and Clinical Implication from a Meta‐Analysis of 8429 Participants

Zhang, Baoliang; Chen, Guanghui; Gao, Xingshuai; Chen, Zhongqiang

doi:10.1111/os.12938

Cited by 4 publications

(1 citation statement)

References 48 publications

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“…OPLL is a pathological condition consisting of heterotopic bone formation within the spinal ligament, leading to spinal-cord compression and neurological symptoms (Zhang et al, 2021). Previous studies have investigated the osteogenic characteristics of spinal ligament fibroblasts in patients with OPLL, yet its pathogenesis is still not fully understood (Ohara, 2018;Yan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Uniaxial cyclic stretch enhances osteogenic differentiation of OPLL-derived primary cells via YAP-Wnt/β-catenin axis

Zhu¹,

Tang²,

He³

et al. 2023

eCM

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The pathogenesis of posterior longitudinal ligament ossification (OPLL) remains inadequately understood. Mechanical stimulation is one of the important pathogenic factors in OPLL. As one of the mechanical stimulation transduction signals, the yes-associated protein (YAP) interacts with the Wnt/β-catenin signalling pathway, which plays an important role in osteogenic differentiation. This study aimed to demonstrate the role of YAP-Wnt/β-catenin axis in cell differentiation induced by mechanical stress. Primary cells extracted from posterior longitudinal ligament tissues from OPLL or non-OPLL patients were subjected to sinusoidal uniaxial cyclic stretch (5 %, 0.5 Hz, 3 d). The expression of runt-related transcription factor 2, collagen I, osterix, osteocalcin and alkaline phosphatase were compared between the static and the experimental groups. In addition, the cytoskeleton was detected using phalloidin staining while YAP phosphorylation states and nuclear location were identified using immunofluorescence. The results showed that mechanical stretching loading increased the expression of osteogenic genes and proteins in the OPLL group, while it had no significant effect on the control group. When OPLL cells were stretched, YAP exhibited an obvious nuclear translocation and the Wnt/β-catenin pathway was activated. Knocking down YAP or β-catenin could weaken the impact upon osteogenic differentiation induced by mechanical stimulation. YAP-mediated mechanical stimulation promoted osteogenic differentiation of OPLL cells through Wnt/β-catenin pathway and this progress was independent of the Hippo pathway.

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Section: Introductionmentioning

confidence: 99%

Uniaxial cyclic stretch enhances osteogenic differentiation of OPLL-derived primary cells via YAP-Wnt/β-catenin axis

Zhu¹,

Tang²,

He³

et al. 2023

eCM

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Effect of K-line (−) or (+) on surgical outcomes in cervical ossification of the posterior longitudinal ligament: A systematic and meta-analysis

Wang,

Chen,

Luo

et al. 2024

Medicine

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Background: To conduct a systematic review and meta-analysis assessing the influence of K-line status on postoperative clinical outcomes in patients with cervical posterior longitudinal ligament ossification (OPLL). Methods: We searched PubMed, Embase, OVID, Cochrane Library, and Web of Science databases for English-language literature related to K-line and OPLL up to June 7, 2024. The study underwent rigorous selection, quality assessment, and data extraction, followed by meta-analysis using Review Manager 5.3 and Stata 17.0 software. This study has been registered with the international PROSPERO registry (registration number: CRD42024558578). Results: Seventeen articles encompassing 1247 patients were included. The meta-analysis revealed that patients with a positive K-line (K-line (+)) exhibited significantly enhanced Japanese Orthopaedic Association score recovery rate (mean differences [MD] = 19.28, 95% confidence interval [CI]: 12.28–26.28, P < .00001), Japanese Orthopaedic Association score (MD = 1.51, 95% CI: 0.64–2.37, P = .0007), C2-C7 Cobb angle (MD = 6.45, 95% CI: 3.73–9.17, P < .00001), alongside reduced occupation ratio (MD = −7.67, 95% CI: −10.86 to −4.49, P < .00001), C2-C7 sagittal vertical axis (MD = −6.18, 95% CI: −8.36 to −3.99, P < .00001), and neck disability index (MD = −1.85, 95% CI: −3.18 to −0.52, P = .006) at final follow-up compared to those with a negative K-line (K-line (−)). No significant differences were observed in postoperative complication rates (odds ratio = 1.15, 95% CI: 0.41–3.20, P = .79), final follow-up visual analog scale scores (MD = −0.10, 95% CI: −0.80 to 0.59, P = .77), or T1 slope (MD = 2.66, 95% CI: −0.20 to 5.52, P = .07) between the 2 groups. Conclusion: A positive preoperative K-line (K-line (+)) is a better predictor of prognosis and is associated with improved clinical outcomes in patients with multi-segmental cervical OPLL compared to a negative K-line (K-line (−)).

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Ossification of the nuchal ligament

Fletke,

Perlman

2024

BMJ Case Rep

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Potential Link between Ossification of Nuchal Ligament and the Risk of Cervical Ossification of Posterior Longitudinal Ligament: Evidence and Clinical Implication from a Meta‐Analysis of 8429 Participants

Cited by 4 publications

References 48 publications

Uniaxial cyclic stretch enhances osteogenic differentiation of OPLL-derived primary cells via YAP-Wnt/β-catenin axis

Uniaxial cyclic stretch enhances osteogenic differentiation of OPLL-derived primary cells via YAP-Wnt/β-catenin axis

Effect of K-line (−) or (+) on surgical outcomes in cervical ossification of the posterior longitudinal ligament: A systematic and meta-analysis

Ossification of the nuchal ligament

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