Potential modulation pancreas gland activity in hyperprolactimic male Rats ( Rattus norvegicus) by vitamin D3 treatment

Abstract

Hyperprolactinemia status is commonly known as abnormal levels of prolactin hormone inthe blood due to endocrine disorder. So far, there is no evidence explaining whetherhyperprolactinemia affected Pancreas gland and vitamin D level in male. Therefore, this study aimedto eliminate hyperprolactinemia affecting the Pancreas gland by treating it with vitamin Dsupplements. Eighteen male rats were equally divide into three groups: The first group (6 rats):received normal saline for 42 days. The second group (6 rats): rats were given 5 mg/kgmetoclopramide by intraperitoneal injection for hyperprolactinemia induction for 14 days. The thirdgroup (6 rats): hyperprolactinemic rats received 2.5 mg/kg vitamin D3 for 28 days.After the end of experimental) 42days(,hormonal parameters (prolactin, insulin, and vitamin D3)were measure, and the Pancreas gland was remove for routine paraffin-embedded section stainingwith hematoxylin and eosin staining. The result of the study revealed a significant decrease (P≤0. 01)in prolactin hormone concentration and a significant increase (P≤0. 01) in D3 concentration andinsulin hormone Group 3compared with Group2. Histological examination of parts of the pancreasgland treated with vitamin D showed a remarkable recovery in the size and number of cells, and thecells became abundant in the cytoplasm. It was also noted that the nuclei returned to a size close tonormal and took on a regular, spherical shape and central location compared to thehyperprolactinemia group. The study concluded that vitamin D had a protective effect on Pancreasgland by stabilizing insulin hormone level and restoration histological architectures throughoutpancreas gland.