Potential of ligninolytic enzymatic complex produced by white‐rot fungi from genus Trametes isolated from Bulgarian forest soil

Abstract

Because of the crucial role of ligninolytic enzymes in a variety of industrial processes, the demand for a new effective producer has been constantly increasing. Furthermore, information on enzyme synthesis by autochthonous fungal strains is very seldom found. Two fungal strains producing ligninolytic enzymes were isolated from Bulgarian forest soil. They were identified as being Trametes trogii and T. hirsuta. These two strains were assessed for their enzyme activities, laccase (Lac), lignin peroxidase (LiP) and Mn‐dependent peroxidase (MnP) in culture filtrate depending on the temperature and the type of nutrient medium. T. trogii was selected as the better producer of ligninolytic enzymes. The production process was further improved by optimizing a number of parameters such as incubation time, type of cultivation, volume ratio of medium/air, inoculum size and the addition of inducers. The maximum activities of enzymes synthesized by T. trogii was detected as 11100 U/L for Lac, 2.5 U/L for LiP and 4.5 U/L for MnP after 14 days of incubation at 25°C under static conditions, volume ratio of medium/air 1:6, and 3 plugs as inoculum. Among the supplements tested, 5% glycerol increased Lac activity to a significant extent. The addition of 1% veratryl alcohol had a positive effect on MnP.

Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage

“…The laccase model is a mini-max function with stationary points at the MS concentration of 16.6 %, substrate moisture of 67.9 %, the temperature of 24.5 °C, and pH 6.4. The contour plots show that high laccase activity could be obtained at 5 ; however, the optimal temperature depends on the fungal species or strain, and it can vary from 25 °C (recorded for some species such as Trametes hirsuta 5,18 ) to 35 °C (for species like T. trogii 18 ). According to the studies conducted by Humphrey and Siggers, and Herrick 19,20 , S. gausapatum is a species that grows at lower temperature.…”

Review Hidrolisis Biomasa Lignoselulosa Untuk Xilitol

“…Enzim yang pertama kali terdeteksi adalah lakase baru kemudian Mangan peroksidase (MnP) dan lignin peroksidase (LiP) (Widiastuti et al, 2008). Penambahan induser seperti 1 % veratril alkohol diketahui mampu meningkatkan produksi MnP dan 5 % gliserol mampu meningkatkan aktivitas dari lakase yang dihasilkan oleh jamur pelapuk putih dari genus Trametes (Krumova et al, 2018). Mekanisme hidrolisis oleh enzim lignolitik berbeda antara satu dengan yang lain.…”

Haploid Genome Analysis Reveals a Tandem Cluster of Four HSP20 Genes Involved in the High-Temperature Adaptation of Coriolopsis trogii

“… Coriolopsis trogii , previously known as Trametes trogii ( https://www.mycoguide.com/guide/fungi/basi/agar/poly/poly/trma/trogii ), is a globally distributed white-rot basidiomycete that has been recognized as an excellent source of ligninolytic enzymes and thermostable laccases ( 1 – 6 ). The crude extract and exopolysaccharides of C. trogii also show strong pharmacological effects, such as anticancer and antioxidative effects ( 7 – 9 ).…”