Potential of nucleofected human MSCs for insulin secretion

Kim, Jae Hyung; Shin, Kyoo-Ho; Li, Tian Zhu; Suh, Hwal

doi:10.1002/term.371

Cited by 11 publications

(6 citation statements)

References 30 publications

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“…The reason does not only lie in the lack of a special marker of mGSC, but also in the small quantity of this kind of cells in testis. Bellvé (1993) isolated mouse mGSC with the purity of 90 % successfully, while Kim selected and obtained pig mGSC with a purity of 80 % (Kim et al 2011). However, there are few reports focusing on the enrichment of bovine mGSC.…”

Section: Discussionmentioning

confidence: 99%

Bovine male germline stem-like cells cultured in serum- and feeder-free medium

Zhuang

et al. 2016

Cytotechnology

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Male germline stem cells (mGSCs) presented in male testis are responsible for spermatogenesis during their whole life. However, little information can be found on the culture of bovine mGSCs, and the current culture system needs to be improved. In this study, we compared the effects of several commercial serum-free media and different extra-cellular matrix on the enrichment and cultivation of mGSCs. To find out the best culture condition, the biological characteristics of the cultured cells were evaluated by morphological observation, RT-PCR and immunofluorescent staining. According to the cells' condition in different experiment groups, we found out an efficient cultivation system for bovine mGSCs derived from neonate testis. In this serum- and feeder-free medium, the cultured cells maintained the typical morphology, and expressed specific surface markers of both pluripotent ES cells and mGSCs, including SSEA-1, CD49f, C-MYC, PLZF, GFRα1, LIN28, NANOG, Oct4 and SOX2 in commercial human ESCs medium PeproGrow-hESC + BIO (6-bromoindirubin-3'-oxime). Embryoid bodies, derived from the bovine mGSCs, and were formed by ganging drop culture. The retinoic acid induced bovine mGSCs were positive for Stra8, SCP3, DZAL, EMA1 and VASA, and resembled spermatid cells morphologically. Thus, we found an efficient bovine mGSCs-cultivation system, which is lack in serum and feeder.

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Section: Discussionmentioning

confidence: 99%

Bovine male germline stem-like cells cultured in serum- and feeder-free medium

Zhuang

et al. 2016

Cytotechnology

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“…It is generally known that mesenchymal stem cells have great differentiation potential and plasticity into different cell lineages (Kim et al ., ), so that many expect that transplantation of the cells may become a good alternative for curing myocardiac infarction. Lineage specification of naive stem cells induced by soluble stimuli has been well described by several groups (Deng et al ., , Hofstetter et al ., , McBeath et al ., , Pittenger et al ., ) and numerous researchers have reported on the ability of stem cells to differentiate into cardiomyocytes, both in vitro and in vivo (Assmus et al ., ; Makino et al ., 1999; Guan et al ., ; Murry et al ., ; Orlic et al ., ; Perin et al ., ; Rangappa et al ., ; Stamm et al ., ; Strauer et al ., ; Tomita et al ., ; Xu et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Influence of in vitro biomimicked stem cell ‘niche’ for regulation of proliferation and differentiation of human bone marrow-derived mesenchymal stem cells to myocardial phenotypes: serum starvation without aid of chemical agents and prevention of spontan

Kim

Shin

et al. 2013

J Tissue Eng Regen Med

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Niche appears important for preventing the spontaneous differentiation or senescence that cells undergo during in vitro expansion. In the present study, it was revealed that human bone marrow-derived mesenchymal stem cells (hBM-MSCs) undergo senescence-related differentiation into the myocardial lineage in vitro without any induction treatment. This phenomenon occurred over the whole population of MCSs, much different from conventional differentiation with limited frequency of occurrence, and was accompanied by a change of morphology into large, flat cells with impeded proliferation, which are the representative indications of MSC senescence. By culturing MSCs under several culture conditions, it was determined that induction treatment with 5-azacytidine was not associated with the phenomenon, but the serum-starvation condition, under which proliferation is severely hampered, caused senescence progression and upregulation of cardiac markers. Nevertheless, MSCs gradually developed a myocardial phenotype under normal culture conditions over a prolonged culture period and heterogeneous populations were formed. In perspectives of clinical applications, this must be prevented for fair and consistent outcomes. Hence, the biomimetic 'niche' was constituted for hBM-MSCs by cultivating on a conventionally available extracellular matrix (ECM). Consequently, cells on ECM regained a spindle-shape morphology, increased in proliferation rate by two-fold and showed decreased expression of cardiac markers at both the mRNA and protein levels. In conclusion, the outcome indicates that progression of MSC senescence may occur via myocardial differentiation during in vitro polystyrene culture, and this can be overcome by employing appropriate ECM culture techniques.

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“…complex retroviruses (Coffin et al, 1997;Walther and Stein, 2000;Anjos-Afonso et al, 2004;Haleem-Smith et al, 2005;Oh et al, 2006;Talens-Visconti et al, 2006;Zaragosi et al, 2007;Li and Lu, 2009;Sugii et al, 2010;Coutu et al, 2011;Kim et al, 2011;Li et al, 2011b).…”

Section: Discussionunclassified

Genetically Modified Adipose Tissue-Derived Stem/Stromal Cells, Using Simian Immunodeficiency Virus-Based Lentiviral Vectors, in the Treatment of Hemophilia B

et al. 2013

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Hemophilia is an X-linked bleeding disorder, and patients with hemophilia are deficient in a biologically active coagulation factor. This study was designed to combine the efficiency of lentiviral vector transduction techniques with murine adipose tissue-derived stem/stromal cells (mADSCs) as a new method to produce secreted human coagulation factor IX (hFIX) and to treat hemophilia B. mADSCs were transduced with simian immunodeficiency virus (SIV)-hFIX lentiviral vector at multiplicities of infection (MOIs) from 1 to 60, and the most effective dose was at an MOI of 10, as determined by hFIX production. hFIX protein secretion persisted over the 28-day experimental period. Cell sheets composed of lentiviral vector-transduced mADSCs were engineered to further enhance the usefulness of these cells for future therapeutic applications in transplantation modalities. These experiments demonstrated that genetically transduced ADSCs may become a valuable cell source for establishing cell-based gene therapies for plasma protein deficiencies, such as hemophilia.

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Potential of nucleofected human MSCs for insulin secretion

Cited by 11 publications

References 30 publications

Bovine male germline stem-like cells cultured in serum- and feeder-free medium

Bovine male germline stem-like cells cultured in serum- and feeder-free medium

Influence of in vitro biomimicked stem cell ‘niche’ for regulation of proliferation and differentiation of human bone marrow-derived mesenchymal stem cells to myocardial phenotypes: serum starvation without aid of chemical agents and prevention of spontan

Genetically Modified Adipose Tissue-Derived Stem/Stromal Cells, Using Simian Immunodeficiency Virus-Based Lentiviral Vectors, in the Treatment of Hemophilia B

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