Potential of Trichoderma spp. and Pinus sylvestris Bark Extracts as Biocontrol Agents against Fungal Pathogens Residing in the Botryosphaeriales

Karličić, Vera; Jovičić-Petrović, Jelena; Marojević, Veljko; Zlatković, Milica; Orlović, Saša; Raičević, Vera

doi:10.3390/iecf2020-07960

Cited by 5 publications

(8 citation statements)

References 29 publications

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“…Practical management options for B. dothidea and other members of the Botryosphaeriaceae family are limited. Biological control methods against the disease caused by these fungi are being developed, but Botryosphaeriaceae invade xylem vessels thus making the application of pesticides or biological control products difficult or even inefficient (Aćimović et al 2019;Karličić et al 2020).…”

Section: Variablesmentioning

confidence: 99%

Comparing environmental impacts of alien plants, insects and pathogens in protected riparian forests

Lapin¹,

Bacher²,

Cech³

et al. 2021

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The prioritization of alien species according to the magnitude of their environmental impacts has become increasingly important for the management of invasive alien species. In this study, we applied the Environmental Impact Classification of Alien Taxa (EICAT) to classify alien taxa from three different taxonomic groups to facilitate the prioritisation of management actions for the threatened riparian forests of the Mura-Drava-Danube Biosphere Reserve, South East Europe. With local experts we collated a list of 198 alien species (115 plants, 45 insects, and 38 fungi) with populations reported in southeast European forest ecosystems and included them in the EICAT. We found impact reports for 114 species. Eleven of these species caused local extinctions of a native species, 35 led to a population decrease, 51 to a reduction in performance in at least one native species and for 17 alien species no effects on individual fitness of native species were detected. Fungi had significantly highest impact and were more likely to have information on their impacts reported. Competition and parasitism were the most important impact mechanisms of alien species. This study is, to our knowledge, the first application of EICAT to all known alien species of several taxonomic groups in a protected area. The impact rankings enabled to identify taxa that generally cause high impacts and to prioritize species for the management in protected areas according to their impact magnitudes. By following a standardized impact protocol, we identified several alien species causing high impacts that do not appear on any expert-based risk list, which are relevant for policymakers. Thus, we recommend that alien species be systematically screened to identify knowledge gaps and prioritize their management with respect to spatio-temporal trends in impact magnitudes.

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Section: Variablesmentioning

confidence: 99%

Comparing environmental impacts of alien plants, insects and pathogens in protected riparian forests

Lapin¹,

Bacher²,

Cech³

et al. 2021

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“…Pinus sylvestris bark extracts were prepared, and extraction yields calculated as described in Karličić et al [22]. The water extracts were obtained at two temperatures (80 and 120 • C) and pH regimes (7 and 9) in three repetitions.…”

Section: Preparation Of Pinus Sylvestris Bark Extractsmentioning

confidence: 99%

“…The water extracts were obtained at two temperatures (80 and 120 • C) and pH regimes (7 and 9) in three repetitions. The extraction yields represented a percentage of soluble bark powder and were 4% for extracts prepared at 80 • C and 5% for extracts prepared at 120 • C [22].…”

Section: Preparation Of Pinus Sylvestris Bark Extractsmentioning

confidence: 99%

“…The putative biocontrol fungal agents Trichoderma spp. were isolated from P. sylvestris bark using a serial dilution in a previous study by Karličić et al [22] and kept in 20% glycerol at −80 • C until use. Morphological identification of the fungal isolates based on colony appearance and microscopic examination (Leica DMLS, Leica Microsystems GmbH, Wetzlar, Germany) was determined after 3-day incubation on PDA at 25 • C in darkness.…”

Section: Fungal Isolatesmentioning

confidence: 99%

“…as BCA of Botryosphaeriaceae has been limited and mostly focused on grapevine trunk diseases [21]. Moreover, except for a preliminary study [22], there has been no previous research related to pine bark extracts as a biological control option for fungi classified in the Botryosphaeriaceae.…”

Section: Introductionmentioning

confidence: 99%

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Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae

Karličić

Zlatković

Jovičić-Petrović

et al. 2021

Forests

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Pinus sylvestris bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of P. sylvestris bark against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Botryosphaeriaceae) through its chemical (water extracts) and biological (Trichoderma spp. isolated from the bark) components. The water bark extracts were prepared at two temperatures (80 and 120 °C) and pH regimes (7 and 9). The presence of bark extracts (30%) caused inhibition of mycelial growth of B. dothidea and D. sarmentorum for 39 to 44% and 53 to 60%, respectively. Moreover, we studied the antagonistic effect of three Trichoderma isolates originating from the pine bark. Trichoderma spp. reduced growth of B. dothidea by 67%–85%, D. sarmentorum by 63%–75% and N. parvum by 55%–62%. Microscopic examination confirmed typical mycoparasitism manifestations (coiling, parallel growth, hook-like structures). The isolates produced cellulase, β-glucosidase and N-acetyl-β-glucosaminidase. The volatile blend detected the emission of several volatile compounds with antimicrobial activity, including nonanoic acid, cubenene, cis-α-bergamotene, hexanedioic acid, and verticillol. The present study confirmed in vitro potential of P. sylvestris bark extracts and Trichoderma spp. against the Botryosphaeriaceae. The study is an important step towards the use of environmentally friendly methods of Botryosphaeriaceae disease control.

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Botryosphaeria Dothidea and Neofusicoccum Yunnanense Causing Canker and Die-Back of Sequoiadendron Giganteum in Croatia

Kovač

Diminić

Orlović

et al. 2021

Forests

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Sequoiadendron giganteum Lindl. [Buchholz] is a long-lived tree species endemic to the Sierra Nevada Mountains in California. Due to its massive size and beauty, S. giganteum is a popular ornamental tree planted in many parts of the world, including Europe. Since 2017, scattered branch die-back has been observed on S. giganteum trees in Zagreb, Croatia. Other symptoms included resinous branch cankers, reddish-brown discoloration of the sapwood and, in severe cases, crown die-back. Branches showing symptoms of die-back and cankers were collected from six S. giganteum trees in Zagreb and the aim of this study was to identify the causal agent of the disease. The constantly isolated fungi were identified using morphology and phylogenetic analyses based on the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA), and partial sequencing of two housekeeping genes, i.e., translation elongation factor 1-α (TEF 1-α), and β tubulin 2 (TUB2). The fungi were identified as Botryosphaeria dothidea (Moug.) Ces. and De Not. and Neofusicoccum yunnanense G.Q. Li & S.F. Chen. The pathogenicity test was conducted in a plant growth chamber on S. giganteum seedlings and revealed that N. yunnanense was more aggressive compared to B. dothidea. N. yunnanense was able to reproduce symptoms of canker and die-back and kill plants seven weeks after inoculation whereas B. dothidea produced cankers. To the best of our knowledge, this is the first report of B. dothidea and N. yunnanense causing canker and die-back disease of S. giganteum in Croatia. It is also the first record on the identity and pathogenicity of any fungal species associated with S. giganteum in this country. The study expended the known host range of N. yunnanense to include S. giganteum, which is a valuable ornamental tree in Croatian landscapes. Disease management strategies should be developed to mitigate or reduce the impact of the disease.

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Potential of Trichoderma spp. and Pinus sylvestris Bark Extracts as Biocontrol Agents against Fungal Pathogens Residing in the Botryosphaeriales

Cited by 5 publications

References 29 publications

Comparing environmental impacts of alien plants, insects and pathogens in protected riparian forests

Comparing environmental impacts of alien plants, insects and pathogens in protected riparian forests

Trichoderma spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae

Botryosphaeria Dothidea and Neofusicoccum Yunnanense Causing Canker and Die-Back of Sequoiadendron Giganteum in Croatia

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