Potential probiotic characteristics and genomic analysis of a new folate‐producing lactic acid bacteria Lactiplantibacillus plantarum ZFM55

Abstract

BackgroundFolate is crucial for maintaining the health, but humans are unable to synthesize folate and need to obtain it from food. Lactiplantibacillus plantarum can produce the necessary vitamin B for the human body, including folate. Whole genome sequencing technology can clarify the physiological characteristics of folate production in L. plantarum. In order to explore new L. plantarum that produce folate, the folate production and probiotic characteristics of L. plantarum ZFM55 isolated from infant feces were investigated, and whole genome sequencing was performed.ResultsThe folate synthesis ability of L. plantarum ZFM55 were measured, and its total folate production was 299.72 ± 28.81 ng/mL. Subsequently, its probiotic properties were explored. The antibacterial test showed that its inhibition zone diameter against Staphylococcus aureus and Salmonella typhimurium was 15.5 ± 0.82 mm and 13.88 ± 0.98 mm, respectively. The tolerance test results indicated that it maintained good activity in simulated gastrointestinal tract and bile salt environments. In vitro intestinal simulation experiments had confirmed that L. plantarum ZFM55 can increase the abundance of beneficial bacteria such as Bifidobacteria in the intestine and inhibit the growth of harmful bacteria such as Escherichia_Shigella. Genomic sequencing indicated that the genetic material of L. plantarum ZFM55 contains 1 chromosome and 3 plasmids, and it has 20 genes related to folate synthesis, which explains its ability to produce folate.ConclusionThis study reports a new potential probiotic that produces folate, and provides ideas for exploring probiotics with specific probiotic characteristics.This article is protected by copyright. All rights reserved.