Potential prophylactic effect of recombinant bovine somatotropin (rbST) in sheep with experimentally induced hyperketonemia

Araújo, Carolina Akiko Sato Cabral; Sousa, Rejane Santos; Monteiro, Bruno Moura; Oliveira, Francisco Leonardo Costa; Minervino, Antônio Humberto Hamad; Rodrigues, Frederico Augusto Mazzocca Lopes; Vale, Rodolfo Gurgel; Mori, Clara Satsuki; Ortolani, Enrico Lippi

doi:10.1016/j.rvsc.2018.06.005

Cited by 7 publications

(11 citation statements)

References 13 publications

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“…Biochemical analyses were performed using specific commercial diagnostic kits as follows: glucose (Sigma Diagnostics Inc. ). The biochemical analyses were performed through an automatic biochemical analyzer (Liasic, AMS Alliance, Rome, Italy) according to protocols published elsewhere [21,22]. The serum globulin concentration was determined as the difference between total protein and albumin concentrations.…”

Section: Methodsmentioning

confidence: 99%

Metabolic Profile of Steers Subjected to Normal Feeding, Fasting, and Re-Feeding Conditions

Ortolani

Maruta

Júnior

et al. 2020

Veterinary Sciences

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The effects of feeding, fasting, and re-feeding on the metabolic and ruminal profiles of growing cattle were studied. Blood and urine samples were obtained from 12 crossbred steers weighing approximately 300 kg during the following periods: 11 h of normal feeding (postprandial period), 48 consecutive hours of fasting, followed by 48 h of re-feeding. Compared with the postprandial period, fasting caused the following modifications: moderate hypoglycemia accompanied by remarkable lipolysis detected by the increase in plasma levels of free fatty acids (FFAs); absence of hepatic lipidosis, as there were no changes in aspartate aminotransferase activity or serum cholesterol levels; mild ketogenesis, confirmed by the slight increase of β-hydroxybutyrate (βHB); increased amino acid burn for energy production, verified by the increase in serum urea contents. There were strong positive correlations between the plasma levels of FFAs and βHB (r = 0.68; p < 0.001), fasting duration and FFA concentration (r = 0.92; p < 0.00001), and fasting duration and serum urea (r = 0.52; p < 0.001); there was a negative correlation between fasting duration and blood glucose (r = −0.52; p < 0.0001). During this same period, mild hypovolemia characterized by an increase in intravascular volume deficit was observed. The metabolic condition observed during fasting was completely reversed during re-feeding, except for the temporarily higher proteolysis.

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Section: Methodsmentioning

confidence: 99%

Metabolic Profile of Steers Subjected to Normal Feeding, Fasting, and Re-Feeding Conditions

Ortolani

Maruta

Júnior

et al. 2020

Veterinary Sciences

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“…The end of pregnancy and start of lactation are the periods of highest metabolic demand and consequently, the time of the highest risk of occurrences of ketosis and pregnancy toxemia (PT) in ewes (Ortolani & Benesi, 1989). Pregnancy toxemia is a metabolic disease caused by a negative energy balance that result in excessive lipid metabolism and ketosis (Araújo et al, 2018). Occurs generally in ewes carrying two or more foetuses and has a high mortality rate (Cal-Pereyra et al, 2015b).…”

Section: Introductionmentioning

confidence: 99%

“…Occurs generally in ewes carrying two or more foetuses and has a high mortality rate (Cal-Pereyra et al, 2015b). In obese ewes, the fat storage formed around the rumen predisposes the occurrence of the disease by reducing dry matter intake (Lacetera et al, 2001;Araújo et al, 2018). Consequently, there is a great fat mobilization in an attempt to maintain the animal's energy supply.…”

Section: Introductionmentioning

confidence: 99%

Validation of a handheld β-hydroxybutyrate acid meter to identify hyperketonaemia in ewes

Araújo

Minervino

Sousa

et al. 2020

PeerJ

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Background The end of pregnancy is the period with the highest risk of occurrences of ketosis and pregnancy toxemia due to fat mobilization and increasing non-esterified fatty acids in the liver which are converted in ketone bodies, mainly β-hydroxybutyrate acid (BHB). This ketone body may also become elevated in the bloodstream. The present study validates the use of a handheld meter for determining the blood concentration of BHB and ascertaining the predictive value and accuracy of BHB measurements in diagnosing hyperketonaemia in ewes. Methods A total of 19, non-pregnant, crossbred ewes were subjected to 2 h of intravenous infusion of a saturated BHB solution. Over 6 h of evaluation, 247 blood samples were obtained in 13 sampling moments. The BHB concentration was measured by an enzymatic colorimetric method in an automated biochemical analyzer (gold-standard) and by a handheld meter using an electrochemical enzyme technique. Results There was a high correlation between both methods (r = 0.98; P < 0.001). Considering the blood BHB concentrations range 0.8–1.6 mmol/L for moderate ketosis the handheld meter presented sensitivity and specificity of 0.98 and 0.81, respectively. For severe ketosis (BHB ≥ 1.6 mmol/L) sensitivity and specificity were 0.99 and 0.75, respectively. Thus, the handheld device can be useful for diagnoses of cases of mild or severe pregnancy toxemia at field conditions.

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“…The end of pregnancy and start of lactation are the periods of highest metabolic demand and, consequently, the time of the highest risk of occurrences of ketosis and pregnancy toxaemia (PT) in ewes (Ortolani & Benesi, 1989). Pregnancy toxaemia is a metabolic disease caused by a negative energy balance that result in excessive lipid metabolism and ketosis (Araújo et al, 2018). Occurs generally in ewes carrying two or more foetuses and has a high mortality rate (Cal-Pereyra et al, 2015b).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Validation of a handheld β-hydroxybutyrate acid meter to identify hyperketonaemia in ewes (v0.2)"

2020

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Background. The end of pregnancy is the period with the highest risk of occurrences of ketosis and pregnancy toxaemia due to fat mobilization and increasing non-esterified fatty acids in the liver which are converted in ketone bodies, mainly β-hydroxybutyrate acid (BHB). This ketone body may also become elevated in the bloodstream. The present study validates the use of a handheld meter for determining the blood concentration of BHB and ascertaining the predictive value and accuracy of BHB measurements in diagnosing hyperketonaemia in ewes. Methods. Nineteen, non-pregnant, crossbred ewes were subjected to two hours of intravenous infusion of a saturated BHB solution. Over six hours of evaluation, 247 blood samples were obtained in 13 sampling moments. The BHB concentration was measured by an enzymatic colorimetric method in an automated biochemical analyser (gold-standard) and by a handheld meter using an electrochemical enzyme technique. Results. There was a high correlation between both methods (r = 0.98; P < 0.001). Considering the blood BHB concentrations range 0.8-1.6 mmol/L for moderate ketosis the handheld meter presented sensitivity and specificity of 0.98 and 0.81, respectively. For severe ketosis (BHB ≥ 1.6 mmol/L) sensitivity and specificity were 0.99 and 0.75, respectively. Thus, the handheld device can be useful for diagnoses of cases of mild or severe pregnancy toxaemia at field conditions.

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Potential prophylactic effect of recombinant bovine somatotropin (rbST) in sheep with experimentally induced hyperketonemia

Cited by 7 publications

References 13 publications

Metabolic Profile of Steers Subjected to Normal Feeding, Fasting, and Re-Feeding Conditions

Metabolic Profile of Steers Subjected to Normal Feeding, Fasting, and Re-Feeding Conditions

Validation of a handheld β-hydroxybutyrate acid meter to identify hyperketonaemia in ewes

Peer Review #2 of "Validation of a handheld β-hydroxybutyrate acid meter to identify hyperketonaemia in ewes (v0.2)"

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