Potential Protective Effects of Bioactive Constituents from Chinese Propolis against Acute Oxidative Stress Induced by Hydrogen Peroxide in Cardiac H9c2 Cells

Abstract

Chinese propolis (CP) is known as a health food but its beneficial effects in protecting cardiomyocytes remain elusive. Here, we investigated the effects of CP and its active compounds on hydrogen peroxide (H2O2) induced rats cardiomyocytes (H9c2) oxidative injury. Cell viability decreases induced by H2O2 were mitigated by different CP extracts using various solvents. From these active fractions, six active compounds were separated and identified. Among tested isolated compound, the cytoprotective activities of three caffeates, caffeic acid phenethyl ester (CAPE), benzyl caffeate (BZC), and cinnamyl caffeate (CNC), exerted stronger effects than chrysin, pinobanksin, and 3,4-dimethoxycinnamic acid (DMCA). These three caffeates also increased H9c2 cellular antioxidant potential, decreased intracellular calcium ion ([Ca2+]i) level, and prevented cell apoptosis. Overall, the cardiovascular protective effects of the CP might be attributed to its caffeates constituents (CAPE, BZC, and CNC) and provide evidence for its usage in complementary and alternative medicine.

Bioactive components and mechanisms of Chinese poplar propolis alleviates oxidized low-density lipoprotein-induced endothelial cells injury

“…Finally, the fractions eluted with 40%, 50%, 60%, 65%, 70%, 75%, 80%, and 90% methanol-water solution gave subfractionI, II, III, IV, V, VI, VII and VII, respectively. The chemical constituents of eight subfractions were analyzed by HPLC-DAD/Q-TOF-MS analysis as previously described [ 15 , 16 ].…”

Comparison of Two Components of Propolis: Caffeic Acid (CA) and Caffeic Acid Phenethyl Ester (CAPE) Induce Apoptosis and Cell Cycle Arrest of Breast Cancer Cells MDA-MB-231

“…Recent studies indicate that caffeic acid (CA), and its phenethyl ester (CAPE) are compounds with potent chemopreventive effects, inter alia by cell cycle inhibition and proapoptotic action [ 13 , 14 , 15 , 16 ]. CAPE is a polyphenol which is a component of honeybee propolis.…”

Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro

“…Chen et al [53] have reported similar findings regarding the protective properties of CAPE against H 2 O 2 -induced death using the LDH assay in 661W mouse cells, i.e., CAPE influences inhibition of cell death in a dose-dependent manner. Sun et al [54] used rat cardiomyocytes cells to follow the action of 2 mM H 2 O 2 ; CAPE was protective, increasing the lifespan of the cells under oxidative stress. The antioxidant properties of CAPE have also been confirmed by Chen et al [55], who found that in peripheral blood mononuclear cells CAPE inhibits xanthine oxidase, which, under physiological conditions, is the source of superoxide anions with the ability to inhibit lipid peroxidation.…”