Potential role of Indian long pepper (<i>Piper longum</i> L.) volatiles against free radicals and multidrug resistant isolates

Dash, Madhusmita; Singh, Subhashree; Sahoo, Bhaskar Chandra; Sahoo, Suprava; Sahoo, Rajesh Kumar; Nayak, Sanghamitra; Kar, Basudeba

doi:10.1080/14786419.2021.1975703

Cited by 11 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Clinical and Laboratory Standards Institute (CLSI, 2013) guidelines and following the protocol of Dash et al (2022), the minimum inhibitory concentration (MIC) of extracted EOs against three MDR strains was determined by the broth micro-dilution method. The antimicrobial potential was tested by Mueller Hinton Broth (MHB) against the three MDR strains.…”

Section: Methodsmentioning

confidence: 99%

Bioactivity Screening of Thirty Black Turmeric (Curcuma caesia Roxb.) Essential Oils Against Free Radicals and MDR Isolates

Khuntia

Lenka

Dash

et al. 2023

Pharmacognosy Magazine

Self Cite

View full text Add to dashboard Cite

Background Curcuma caesia Roxb. (Black turmeric) is a perennial medicinal herb belonging to the Zingiberaceae family that is endangered in Southeast Asia. It is treasured for its high-quality essential oil with tremendous medicinal and aromatic properties. In the present scenario, C. caesia Roxb. is an unexplored plant for drug discovery. Objectives The present study was undertaken to compare the bioactivities of Thirty C. caesia rhizomes and leaf oils collected from various eco-regions of Eastern India. Materials and Methods The comparative antioxidant and antimicrobial activities of leaf and rhizome essential oils from different eco-regions of Eastern India were assessed. The antioxidant activities were evaluated against standards like butylated hydroxytoluene (BHT) and ascorbic acid by the 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. Moreover, the essential oils were also evaluated for their antimicrobial activity using the broth micro-dilution assay for minimum inhibitory concentrations (MIC) against multidrug resistant strains. Results Leaf essential oils exhibited a considerable level of antioxidant potential as compared to rhizome essential oils and standard BHT. Furthermore, the essential oils also possessed a significant level of inhibitory activity against three multidrug-resistant (MDR) strains, such as Acinetobacter baumannii, Escherichia coli, and Klebsiella pneumoniae. The rhizome essential oils had shown the most effective antimicrobial activity against A. baumannii (MIC: 0.09 to 6.25 µg/mL) when compared with the positive control, ampicillin (MIC: 25 µg/mL). Conclusion The variability in bioactivities was greatly influenced by geographical origin. The identified accessions of C. caesia, that is, Cc 26 with better bioactivity potential, might be useful for formulating drugs in the future.

show abstract

Section: Methodsmentioning

confidence: 99%

Bioactivity Screening of Thirty Black Turmeric (Curcuma caesia Roxb.) Essential Oils Against Free Radicals and MDR Isolates

Khuntia

Lenka

Dash

et al. 2023

Pharmacognosy Magazine

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to several reports, sesquiterpene hydrocarbons class of compounds dominated the dried fruit oil composition. 6,30,31 However, a study by Varughese et al stated that monoterpene hydrocarbons group was the major one in fresh fruit oil. 29 The major constituent identified was germacrene-D with 2.87% ± 0.01% of the whole oil, followed by the other significant constituents like 8-heptadecene (2.69% ± 0.03%), β-caryophyllene (2.43% ± 0.03%), and nheptadecane (2.40% ± 0.04%) (Table 1).…”

Section: Terpenic Composition Of P Longum Oilmentioning

confidence: 99%

“…5 Numerous studies reported that the observed therapeutic potential may be due to various volatile compounds present in the fruit of this species, which have to be explored by modern chromatographic techniques. 6,7,8,9,10,11,12,13,14,15,16 The onedimensional (1D) gas chromatography-mass spectrometry (GC-MS) has been used efficiently for the analysis of volatile constituents; however, better separation with high resolution of complex as well as coeluting compounds is lacking because of single column. 17 The isomeric nature of certain compounds results in overlapping peaks because they coelute at similar retention time.…”

Section: Introductionmentioning

confidence: 99%

“…The characterization of fruit volatile oil of long pepper has been reported in several reports by 1D GC-MS, indicating the presence of around 50 constituents. 5,6,[29][30][31] The small number of constituents might have been identified due to single non-polar column, as one column was not able to identify multiple overlapping constituents. However, it is envisaged that combining a non-polar and a polar column together might help toward precise separation of the coeluting or overlapping constituents.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolite profiling of Piper longum L. fruit volatiles by two‐dimensional gas chromatography and time‐of‐flight mass spectrometry: Insights into the chemical complexity

Dash,

Singh,

Sahoo

et al. 2024

Biotech and App Biochem

Self Cite

View full text Add to dashboard Cite

Piper longum L. (long pepper) is an economically and industrially important medicinal plant. However, the characterization of its volatiles has only been analyzed by gas chromatography–mass spectrometry (GC–MS). In the present study, precise characterization of P. longum fruit volatiles has been performed for the first time through advanced two‐dimensional gas chromatography–time‐of‐flight spectrometry (GC×GC–TOFMS). A total of 146 constituents accounting for 93.79% were identified, of which 30 were reported for the first time. All these constituents were classified into alcohols (4.5%), alkanes (8.9%), alkenes (6.71%), esters (6.15%), ketones (0.58%), monoterpene hydrocarbons (1.64%), oxygenated monoterpenes (2.24%), sesquiterpene hydrocarbons (49.61%), oxygenated sesquiterpenes (13.03%), phenylpropanoid (0.23%), and diterpenes (0.2%). Among all the classes, sesquiterpene hydrocarbons were abundant, with germacrene‐D (2.87% ± 0.01%) as the major one, followed by 8‐heptadecene (2.69% ± 0.03%), β‐caryophyllene (2.43% ± 0.03%), n‐heptadecane (2.4% ± 0.04%), n‐pentadecane (2.11% ± 0.05%), and so forth. Further, 20 constituents were observed to be coeluted and separated precisely in the two‐dimensional column. The investigation provides an extensive metabolite profiling of P. longum fruit volatiles, which could be helpful to improve its therapeutic potential.

show abstract

“…GC-MS analysis is one of the most appropriate and trustworthy systems broadly used for the identification and determination of volatile phytoconstituents. Various researchers reported the occurrence of diverse phytochemicals in many species using this technique (Sahoo et al, 2014;Sahoo et al, 2017;Sahoo et al, 2020;Dash et al, 2021;Singh et al, 2021). Likewise, the phytoconstituents of the rhizome essential oil of K. galanga have been studied by several researchers (Ravindran & Balachandran, 2005;Tewtrakul et al, 2005;Mohanty et al, 2011;Sahoo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of Kaempferia galanga leaf and rhizome extract using GC-MS

Khuntia

Sahoo²,

Kar³

et al. 2022

jaar

Self Cite

View full text Add to dashboard Cite

Kaempferia galanga Linn. is an endangered rhizomatous medicinal plant belonging to the Zingiberaceae family. It has evolved as an emerging industrial crop and dominates pharma as well as aroma sector. Though the extracts of this species have been extensively used in herbal medicine across the globe for the treatment of numerous diseases, but still the composition of the extract is not characterized properly. Thus, methanol extracts of K. galanga leaves and rhizomes were subjected to phytochemical screening, total phenolic content (TPC), total flavonoid content (TFC), and Gas Chromatography-Mass Spectrometry (GC-MS) to analyze the phytoconstituents. Leaf extract contained more TPC and TFC values as compared to rhizome extract. A total of eight and ten compounds were identified in the leaf and rhizome extract accounting for 61.44% and 96.97% of the total peak area respectively. Ethyl p-methoxycinnamate was found as the main constituent in rhizome extract covering 80.39% of the total area. Other important compounds like ethyl cinnamate (9.61±0.45%), pentadecane (3.12±0.2%) were also found in the rhizome extract, whereas leaf extract contained 2-(3,4-dimethoxyphenyl)-7-hydroxy-3-methoxy-4H-chromen-4-one (18.26%), 2-(3-hydroxy-4- methoxyphenyl)-3,7-dimethoxy-4H-chromen-4-one (14.01%), octamethylcyclotetrasiloxane (11.79%). The study indicated that K. galanga is a good source of phytoconstituents which can be used at the industrial level to produce pharmaceuticals, perfumes and flavouring agents.

show abstract

Potential role of Indian long pepper (Piper longum L.) volatiles against free radicals and multidrug resistant isolates

Cited by 11 publications

References 12 publications

Bioactivity Screening of Thirty Black Turmeric (Curcuma caesia Roxb.) Essential Oils Against Free Radicals and MDR Isolates

Bioactivity Screening of Thirty Black Turmeric (Curcuma caesia Roxb.) Essential Oils Against Free Radicals and MDR Isolates

Metabolite profiling of Piper longum L. fruit volatiles by two‐dimensional gas chromatography and time‐of‐flight mass spectrometry: Insights into the chemical complexity

Metabolic profiling of Kaempferia galanga leaf and rhizome extract using GC-MS

Contact Info

Product

Resources

About