Potential Roles of Environmental and Socio-Economic Factors in the Distribution of Insecticide Resistance in Anopheles gambiae sensu lato (Culicidae: Diptera) Across Togo, West Africa

Ahadji-Dabla, Koffi Mensah; Romero-Álvarez, Daniel; Djègbè, Innocent; Amoudji, Adjovi D.; Apétogbo, Georges Yawo; Djouaka, Rousseau; Oboussoumi, Komlanvi; Aawi, Agnidoufèyi; Atcha-Oubou, Tinah; Peterson, A. Townsend; Ketoh, Guillaume Koffivi

doi:10.1093/jme/tjaa023

Cited by 6 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, An. arabiensis is found in Côte d'Ivoire's neighbouring countries, including Burkina Faso, Mali and Ghana [38][39][40] and across the West Africa region in countries, such as, Benin [41], Cabo Verde [42], Gambia [43], Guinea Bissau [44], Mauritania [45], Nigeria [46], Senegal [47] and Togo [48]. Anopheles arabiensis generally breeds and predominates in semi-arid and savannah areas [49,50].…”

Section: Discussionmentioning

confidence: 99%

Species composition and insecticide resistance in malaria vectors in Ellibou, southern Côte d’Ivoire and first finding of Anopheles arabiensis in Côte d’Ivoire

N'Dri

Wipf

Saric

et al. 2023

Malar J

View full text Add to dashboard Cite

Background Knowing the species composition and insecticide resistance status of the target vector population is important to guide malaria vector control. The aim of this study was to characterize the malaria vector population in terms of species composition, insecticide susceptibility status and potential underlying resistance mechanisms in Ellibou, southern Côte d’Ivoire. Methods A 1-year longitudinal entomological survey was conducted using light traps and pyrethroid spray catches to sample adult mosquitoes in combination with larval sampling. The susceptibility status of Anopheles gambiae sensu lato (s.l.) to bendiocarb, deltamethrin, DDT and malathion was assessed using the World Health Organization insecticide susceptibility test. Additionally, An. gambiae specimens were screened for knockdown (kdr) and acetylcholineesterase (ace1) target site resistance alleles, and the expression levels of eight metabolic resistance genes, including seven cytochrome P450 monooxygenases (P450s) and one glutathione S-transferase (GST), measured with reverse transcription quantitative real-time polymerase chain reaction (qPCR). Results Overall, 2383 adult mosquitoes from 12 different taxa were collected with Culex quinquefasciatus and An. gambiae being the predominant taxa. Molecular identification of An. gambiae s.l. revealed the presence of Anopheles arabiensis, Anopheles coluzzii, An. gambiae sensu stricto (s.s.) and Anopheles coluzzii/An. gambiae s.s. hybrids. Anopheles gambiae mosquitoes were resistant to all insecticides except malathion. PCR diagnostics revealed the presence of ace1-G280S and the kdr L995F, L995S and N1570Y target-site mutations. Additionally, several genes were upregulated, including five P450s (i.e., CYP6P3, CYP6M2, CYP9K1, CYP6Z1, CYP6P1) and GSTE2. Conclusion This is the first documented presence of An. arabiensis in Côte d’Ivoire. Its detection – together with a recent finding further north of the country – confirms its existence in the country, which is an early warning sign, as An. arabiensis shows a different biology than the currently documented malaria vectors. Because the local An. gambiae population was still susceptible to malathion, upregulation of P450s, conferring insecticide resistance to pyrethroids, together with the presence of ace1, suggest negative cross-resistance. Therefore, organophosphates could be an alternative insecticide class for indoor residual spraying in the Ellibou area, while additional tools against the outdoor biting An. arabiensis will have to be considered.

show abstract

Section: Discussionmentioning

confidence: 99%

Species composition and insecticide resistance in malaria vectors in Ellibou, southern Côte d’Ivoire and first finding of Anopheles arabiensis in Côte d’Ivoire

N'Dri

Wipf

Saric

et al. 2023

Malar J

View full text Add to dashboard Cite

show abstract

“…In the same country, high kdr frequency was detected in cotton-growing areas than in only food crops-growing rural areas that are not treated with insecticide [ 8 ]. In a recently published study, three kdr mutations (L1014F, L1014S, and N1575Y) were detected at Nangbeto, a neighboring site [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Resistance intensity status of Anopheles gambiae s.l. species at KOLOKOPE, eastern plateau Togo: A potential site to assess new vector control tools

Ahadji-Dabla¹,

Chabi²,

Apétogbo³

et al. 2022

Heliyon

Self Cite

View full text Add to dashboard Cite

“…gambiae and An. arabiensis, the main malaria vectors, have been recently reported in Togo [29][30][31] .…”

Section: Discussionmentioning

confidence: 99%

Mosquito (Diptera: Culicidae) diversity and malaria prevalence in Kovié, prefecture of Zio, Togo

Toglo¹,

Ahadji-Dabla²,

Koffi³

et al. 2021

Int. J. Mosq. Res.

Self Cite

View full text Add to dashboard Cite

Vector-borne diseases are diseases caused by parasites and viruses transmitted by vectors. Africa is home to a rich diversity of vector species which can transmit various diseases including malaria. Human adaptation strategies to climate change such as irrigation and the development of hydro-agricultural areas create a favorable environment that reinforces the development and proliferation of vectors, particularly Culicidae. To determine the composition of mosquito fauna and the malaria prevalence, series of larval surveys and adult mosquito collections (by Spray catch and Human Landin catch) were conducted in Kovié, a peri-urban locality located in the River Zio basin characterized with irrigated rice production. Results showed that the mosquito fauna at Kovié is primarily made up of Anophelinae (78%) and Culicinae (22%), belonging to 4 genera: Mansonia, Aedes, Culex, and Anopheles. The identification of 813 specimens of Anopheles gambiae s.l. using PCR revealed that Anopheles coluzzii was the only species collected. Epidemiological surveys in Kovié medical center showed that malaria was the only mosquito-borne disease recorded in the area. Malaria prevalence in the locality was more or less high among children under 5 years old and pregnant women.

show abstract

Potential Roles of Environmental and Socio-Economic Factors in the Distribution of Insecticide Resistance in Anopheles gambiae sensu lato (Culicidae: Diptera) Across Togo, West Africa

Cited by 6 publications

References 37 publications

Species composition and insecticide resistance in malaria vectors in Ellibou, southern Côte d’Ivoire and first finding of Anopheles arabiensis in Côte d’Ivoire

Species composition and insecticide resistance in malaria vectors in Ellibou, southern Côte d’Ivoire and first finding of Anopheles arabiensis in Côte d’Ivoire

Resistance intensity status of Anopheles gambiae s.l. species at KOLOKOPE, eastern plateau Togo: A potential site to assess new vector control tools

Mosquito (Diptera: Culicidae) diversity and malaria prevalence in Kovié, prefecture of Zio, Togo

Contact Info

Product

Resources

About