To establish the clinical significance of low-level radiation exposure, this study was conducted under the artificial radioactive circumstances, such as internal exposure of alpha-ray with radon (Rn 222) dissolved water (RDW) intake, and its production method is uncomplicated and presented as public property by the third party. Here, we report that this method provides the wide range of availabilities in radioactivity. This 1-year experimental study investigated the effect of RDW on a dog as a mammal model, resulted in no biochemical harmfulness. To acquire the accuracy of this consequence, the accumulation of more data in mammal models is indespensable with consistent radioactive backgrounds.