Potential Use of a Weak Ethylene Receptor Mutant, <i>Sletr1-2</i>, as Breeding Material To Extend Fruit Shelf Life of Tomato

Mubarok, Syariful; Okabe, Yoshihiro; Fukuda, Naoya; Ariizumi, Tohru; Ezura, Hiroshi

doi:10.1021/acs.jafc.5b02742

Cited by 40 publications

(63 citation statements)

References 45 publications

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“…A drawback of constitutive expression of ethylene receptor mutants that cause ethylene insensitivity is unintended effects which can have adverse agricultural and horticultural outcomes. These adverse outcomes include increased stress in tomato plants, increased pathogen susceptibility in tobacco, reduction in seed germination, pollen viability, number of adventitious roots and root performance in petunias, and reduced femaleness in melon flowers (194,195,(199)(200)(201)(202)(203). These unwanted effects reduce the efficacy of this approach for commercial use.…”

Section: Regulating Ethylene Signal Transduction For Agricultural Andmentioning

confidence: 99%

Ethylene signaling in plants

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2020

Journal of Biological Chemistry

389

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Ethylene is a gaseous phytohormone and the first of this hormone class to be discovered. It is the simplest olefin gas and is biosynthesized by plants to regulate plant development, growth, and stress responses via a well-studied signaling pathway. One of the earliest reported responses to ethylene is the triple response. This response is common in eudicot seedlings grown in the dark and is characterized by reduced growth of the root and hypocotyl, an exaggerated apical hook, and a thickening of the hypocotyl. This proved a useful assay for genetic screens and enabled the identification of many components of the ethylene-signaling pathway. These components include a family of ethylene receptors in the membrane of the endoplasmic reticulum (ER); a protein kinase, called constitutive triple response 1 (CTR1); an ER-localized transmembrane protein of unknown biochemical activity, called ethylene-insensitive 2 (EIN2); and transcription factors such as EIN3, EIN3-like (EIL), and ethylene response factors (ERFs). These studies led to a linear model, according to which in the absence of ethylene, its cognate receptors signal to CTR1, which inhibits EIN2 and prevents downstream signaling. Ethylene acts as an inverse agonist by inhibiting its receptors, resulting in lower CTR1 activity, which releases EIN2 inhibition. EIN2 alters transcription and translation, leading to most ethylene responses. Although this canonical pathway is the predominant signaling cascade, alternative pathways also affect ethylene responses. This review summarizes our current understanding of ethylene signaling, including these alternative pathways, and discusses how ethylene signaling has been manipulated for agricultural and horticultural applications.

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Section: Regulating Ethylene Signal Transduction For Agricultural Andmentioning

confidence: 99%

Ethylene signaling in plants

Binder

2020

Journal of Biological Chemistry

389

300

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“…Lycopene and β-carotene contents in Br+10 fruits were measured as described previously (Kitagawa et al 2005;Mubarok et al 2015). Pigments were extracted from powdered fruits with acetone-hexane (4:6) solution, and the optical density of the supernatant was simultaneously measured in a spectrophotometer at 663 nm (A 663 ), 645 nm (A 645 ), 505 nm (A 505 ), and 453 nm (A 453 ).…”

Section: Determination Of Lycopene and β-Carotene Contentsmentioning

confidence: 99%

Activating glutamate decarboxylase activity by removing the autoinhibitory domain leads to hyper γ-aminobutyric acid (GABA) accumulation in tomato fruit

et al. 2016

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The C-terminal extension region of SlGAD3 is likely involved in autoinhibition, and removing this domain increases GABA levels in tomato fruits. γ-Aminobutyric acid (GABA) is a ubiquitous non-protein amino acid with several health-promoting benefits. In many plants including tomato, GABA is synthesized via decarboxylation of glutamate in a reaction catalyzed by glutamate decarboxylase (GAD), which generally contains a C-terminal autoinhibitory domain. We previously generated transgenic tomato plants in which tomato GAD3 (SlGAD3) was expressed using the 35S promoter/NOS terminator expression cassette (35S-SlGAD3-NOS), yielding a four- to fivefold increase in GABA levels in red-ripe fruits compared to the control. In this study, to further increase GABA accumulation in tomato fruits, we expressed SlGAD3 with (SlGAD3 ) or without (SlGAD3ΔC ) a putative autoinhibitory domain in tomato using the fruit ripening-specific E8 promoter and the Arabidopsis heat shock protein 18.2 (HSP) terminator. Although the GABA levels in SlGAD3 fruits were equivalent to those in 35S-SlGAD3-NOS fruits, GABA levels in SlGAD3ΔC fruits increased by 11- to 18-fold compared to control plants, indicating that removing the autoinhibitory domain increases GABA biosynthesis activity. Furthermore, the increased GABA levels were accompanied by a drastic reduction in glutamate and aspartate levels, indicating that enhanced GABA biosynthesis affects amino acid metabolism in ripe-fruits. Moreover, SlGAD3ΔC fruits exhibited an orange-ripe phenotype, which was associated with reduced levels of both carotenoid and mRNA transcripts of ethylene-responsive carotenogenic genes, suggesting that over activation of GAD influences ethylene sensitivity. Our strategy utilizing the E8 promoter and HSP terminator expression cassette, together with SlGAD3 C-terminal deletion, would facilitate the production of tomato fruits with increased GABA levels.

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“…Pada tanaman tomat teridentifikasi enam gen reseptor etilen, yaitu SlETR1, SlETR2, SlETR3/NR, SlETR4, SlETR5, dan SlETR6 yang menjadi koleksi Micro-tom library Japan, University of Tsukuba dan telah dilakukan mutasi pada gen reseptor SlETR1 sehingga diperoleh Sletr1-1 dan Sletr1-2. Kedua gen tersebut diketahui efektif dalam menekan sensitivitas terhadap etilen sehingga dapat meningkatkan daya simpan buah (Mubarok et al, 2015).…”

Section: Pendahuluanunclassified

Keberhasilan Persilangan Tomat Varietas Komersial (Lycopersicum esculentum L.) dengan Tomat Mutan Tahan Simpan

Wiguna¹,

Rosalita²,

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et al. 2019

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Post-harvest loss is an obstacle for tomato farmers that cause tomato farming being unprofitable. Utilization of mutant genes prolong tomato fruit shelf life through crossing is one of the best solutions to improve the fruit quality. The objective of this study was to determine the success of cross-pollination between commercial tomato varieties and shelf life of mutant tomatoes. Two mutant tomato lines Sletr1-1 and Sletr1-2, and one wild type strain were crossed with four commercial varieties (Intan, Mutiara, Ratna, and Mirah). The results showed that the success rate of crossing ranged from 77.50% to 100%, with an average of 95.76%. The average maximum growth potential of seeds produced was 89.17% with the lowest value of 75% and the highest 98.5%. The most top success of crosses was built by a combination of crossing Ratna x Sletr1-2, and the combination of Mirah x Sletr1-1 showed the lowest percentage of the crossing. The Intan showed the highest success as female parent of crossing, while Mirah was the lowest production of the crossing. The plant height of all hybrid was smaller compared to the commercial varieties.

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Potential Use of a Weak Ethylene Receptor Mutant, Sletr1-2, as Breeding Material To Extend Fruit Shelf Life of Tomato

Cited by 40 publications

References 45 publications

Ethylene signaling in plants

Ethylene signaling in plants

Activating glutamate decarboxylase activity by removing the autoinhibitory domain leads to hyper γ-aminobutyric acid (GABA) accumulation in tomato fruit

Keberhasilan Persilangan Tomat Varietas Komersial (Lycopersicum esculentum L.) dengan Tomat Mutan Tahan Simpan

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