Potentially Mobile Denitrification Genes Identified in
            <i>Azospirillum</i>
            sp. Strain TSH58

Jang, Jeonghwan; Sakai, Yoriko; Senoo, Keishi; Ishii, Satoshi

doi:10.1128/aem.02474-18

Cited by 18 publications

(6 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Magnetospirillum contains genes capable of compiling nitrate reductase, nitrite reductase, nitric oxide reductase, and nitrous oxide reductase, with complete denitrification capability [ 82 ]. Azospirillum has complete denitrification functional genes and can fix nitrogen [ 83 , 84 ]. In the A48 treatment, the dominant bacteria (relative abundance > 3%) were Pseudomonas (61.34%), Diaphorobacter (9.54%), and Lysobacter (4.09%).…”

Section: Resultsmentioning

confidence: 99%

Pyrite-Based Autotrophic Denitrifying Microorganisms Derived from Paddy Soils: Effects of Organic Co-Substrate Addition

Yang

et al. 2022

IJERPH

View full text Add to dashboard Cite

The presence of organic co-substrate in groundwater and soils is inevitable, and much remains to be learned about the roles of organic co-substrates during pyrite-based denitrification. Herein, an organic co-substrate (acetate) was added to a pyrite-based denitrification system, and the impact of the organic co-substrate on the performance and bacterial community of pyrite-based denitrification processes was evaluated. The addition of organic co-substrate at concentrations higher than 48 mg L−1 inhibited pyrite-based autotrophic denitrification, as no sulfate was produced in treatments with high organic co-substrate addition. In contrast, both competition and promotion effects on pyrite-based autotrophic denitrification occurred with organic co-substrate addition at concentrations of 24 and 48 mg L−1. The subsequent validation experiments suggested that competition had a greater influence than promotion when organic co-substrate was added, even at a low concentration. Thiobacillus, a common chemolithoautotrophic sulfur-oxidizing denitrifier, dominated the system with a relative abundance of 13.04% when pyrite served as the sole electron donor. With the addition of organic co-substrate, Pseudomonas became the dominant genus, with 60.82%, 61.34%, 70.37%, 73.44%, and 35.46% abundance at organic matter concentrations of 24, 48, 120, 240, and 480 mg L−1, respectively. These findings provide an important theoretical basis for the cultivation of pyrite-based autotrophic denitrifying microorganisms for nitrate removal in soils and groundwater.

show abstract

Section: Resultsmentioning

confidence: 99%

Pyrite-Based Autotrophic Denitrifying Microorganisms Derived from Paddy Soils: Effects of Organic Co-Substrate Addition

Yang

et al. 2022

IJERPH

View full text Add to dashboard Cite

show abstract

“…Some studies have shown that Azospirillum spp. not only have nitrogen-fixing function, but also have some other functions, such as heavy oil degrading, atrazine degrading, denitrification ability, carotenoids produce ( Jang et al, 2019 ; Liu et al, 2019 ; Wu et al, 2020 ; Mishra et al, 2021 ), and the functional diversity of Azospirillum spp. needs to be studied future.…”

Section: Discussionmentioning

confidence: 99%

Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet

Pan

Zhu

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

A bacterial strain A1-3 with iprodione-degrading capabilities was isolated from the soil for vegetable growing under greenhouses at Lhasa, Tibet. Based on phenotypic, phylogenetic, and genotypic data, strain A1-3 was considered to represent a novel species of genus Azospirillum. It was able to use iprodione as the sole source of carbon and energy for growth, 27.96 mg/L (50.80%) iprodione was reduced within 108 h at 25°C. During the degradation of iprodione by Azospirillum sp. A1-3, iprodione was firstly degraded to N-(3,5-dichlorophenyl)-2,4-dioxoimidazolidine, and then to (3,5-dichlorophenylurea) acetic acid. However, (3,5-dichlorophenylurea) acetic acid cannot be degraded to 3,5-dichloroaniline by Azospirillum sp. A1-3. A ipaH gene which has a highly similarity (98.72–99.92%) with other previously reported ipaH genes, was presented in Azospirillum sp. A1-3. Azospirillum novel strain with the ability of iprodione degradation associated with nitrogen fixation has never been reported to date, and Azospirillum sp. A1-3 might be a promising candidate for application in the bioremediation of iprodione-contaminated environments.

show abstract

“…Similar to this study, association of denitrification genes ( nir ‐ nor cluster) on a composite transposon was recently reported in an Azospirillum sp. denitrifier (Jang et al 2019b). These results suggest that denitrification genes might be horizontally transferred among cells.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of denitrifiers from woodchip bioreactors for bioaugmentation application

et al. 2020

Self Cite

View full text Add to dashboard Cite

Aims: This study was done to obtain denitrifiers that could be used for bioaugmentation in woodchip bioreactors to remove nitrate from agricultural subsurface drainage water. Methods and Results: We isolated denitrifiers from four different bioreactors in Minnesota, and characterized the strains by measuring their denitrification rates and analysing their whole genomes. A total of 206 bacteria were isolated from woodchips and thick biofilms (bioslimes) that formed in the bioreactors, 76 of which were able to reduce nitrate at 15°C. Among those, nine potential denitrifying strains were identified, all of which were isolated from the woodchip samples. Although many nitrate-reducing strains were isolated from the bioslime samples, none were categorized as denitrifiers but instead as carrying out dissimilatory nitrate reduction to ammonium. Conclusions: Among the denitrifiers confirmed by 15 N stable isotope analysis and genome analysis, Cellulomonas cellasea strain WB94 and Microvirgula aerodenitrificans strain BE2.4 appear to be promising for bioreactor bioaugmentation due to their potential for both aerobic and anaerobic denitrification, and the ability of strain WB94 to degrade cellulose. Significance and Impact of the Study: Denitrifiers isolated in this study could be useful for bioaugmentation application to enhance nitrate removal in woodchip bioreactors.

show abstract

Potentially Mobile Denitrification Genes Identified in Azospirillum sp. Strain TSH58

Cited by 18 publications

References 57 publications

Pyrite-Based Autotrophic Denitrifying Microorganisms Derived from Paddy Soils: Effects of Organic Co-Substrate Addition

Pyrite-Based Autotrophic Denitrifying Microorganisms Derived from Paddy Soils: Effects of Organic Co-Substrate Addition

Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet

Isolation and characterization of denitrifiers from woodchip bioreactors for bioaugmentation application

Contact Info

Product

Resources

About