Potentiation between Pentagastrin and Histamine in Stimulation of Secretion from Totally Isolated &lt;i&gt;ex vivo&lt;/i&gt; Canine Stomach

Kowalewski, K.; Kolodej, A.

doi:10.1159/000136310

Cited by 6 publications

(14 citation statements)

References 9 publications

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“…It was also found that bethanechol chloride potentiated the pepsin response o f the ex vivo canine stomach to histamine (17) and pentagastrin (19). These suggestions (11) and observations (17,19) were verified in the present experiment. High (P -O / H C l-O ) ratios were obtained when bethan- echol chloride was infused alone, or combined with histamine or pentagastrin.…”

Section: Resultssupporting

confidence: 84%

“…When combined with histamine or missive cholinergic mechanism may be required for peptic cells to respond fully to histamine (11). It was also found that bethanechol chloride potentiated the pepsin response o f the ex vivo canine stomach to histamine (17) and pentagastrin (19). These suggestions (11) and observations (17,19) were verified in the present experiment.…”

Section: Resultssupporting

confidence: 81%

“…We also determine O , consumption (18). Methods employed and instruments used for these measurements were quoted previously (16,17,(19)(20)(21)(22)(23). In the tables and charts published (1 6 -2 3 ) we reported on a large number o f components o f perfusion blood and gastric secretion, using a variety o f stimulants.…”

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confidence: 99%

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Correlation between the Outputs of Pepsin and HCl by Isolated, ex vivo Perfused, Canine Stomach Stimulated with Humoral and Cholinergic Drugs

Kowalewski¹,

Kolodej²

1973

Pharmacology

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The totally isolated canine stomach, perfused with homologous blood, was chloride used for the study of gastric pepsin and HCl. Secretion was stimulated for several hours by intra-arterial infusion of histamine, pentagastrin and bethanechol chloride, given separately or combined. The ratio of outputs of pepsin and HCl (P-O/HCl-O) were calculated. The relationship between paired values of pepsin (mg/30 min) and H⁺ (mEq/30 min) was analyzed by computer and the linear correlation was calculated between these two values. Bethanechol chloride was found to be a very strong stimulant of pepsin and high (P-O/HCl-O) ratios were found in the presence of cholinergic stimulation applied separately or in combination with the humoral secretagogues used. Bethanechol chloride potentiated the effect of histamine and pentagastrin on pepsin secretion. No such potentiation was observed when histamine was combined with pentagastrin. The relationship between pepsin and acid expressed by (P-O/HCl-O) ratio, was dependent upon the secretagogue used. Linear regression lines satisfactorily characterized the relationship between pepsin and H⁺ outputs under all conditions of stimulation applied in this experiment.

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Section: Resultssupporting

confidence: 84%

Section: Resultssupporting

confidence: 81%

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Correlation between the Outputs of Pepsin and HCl by Isolated, ex vivo Perfused, Canine Stomach Stimulated with Humoral and Cholinergic Drugs

Kowalewski¹,

Kolodej²

1973

Pharmacology

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“…It (16,(21)(22)(23)(24)(25); however, interactions demonstrated in this manner are species dependent and variable in magnitude (15,(26)(27)(28). A dramatic demonstration of secretagogue interactions has been made in studies with the ex vivo canine stomach preparation where marked potentiation was found between histamine and pentagastrin, between histamine and bethanechol, and between pentagastrin and bethanechol (29,30). Further in vivo evidence for interactions between secretagogues comes from studies of secretagogue effect on acid secretion after both vagotomy and antrectomy.…”

Section: Discussionmentioning

confidence: 99%

The Interaction of Histamine with Gastrin and Carbamylcholine on Oxygen Uptake by Isolated Mammalian Parietal Cells

Soll¹

1978

J. Clin. Invest.

164

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A B ST RA CT Using oxygen uptake as an index of the physiological response of isolated parietal cells, the interactions between histamine and gastrin and between histamine and carbamylcholine and the effects of atropine and metiamide on these interactions have been studied. Parietal cells were isolated from canine fundic mucosa by sequential exposure of separated mucosa to collagenase and EDTA. In previous studies carbamylcholine, isobutyl methyl xanthine, gastrin, and histamine have each been shown to increase oxygen uptake by these cells. Isobutyl methyl xanthine greatly enhanced the histamine effect. Carbamylcholine was inhibited by atropine but not by metiamide, histamine was inhibited by metiamide but not by atropine, and gastrin was inhibited by neither, suggesting that each of these agents has a direct action on the parietal cell. In the present studies, potentiating interactions between histamine and carbamylcholine and between histamine and gastrin have been demonstrated. Against a histamine (0.1 and 1 ,M) plus isobutyl methyl xanthine (0.1 mM) background, the dose for 50% response for gastrin was approximately 1 nM, and the maximal response was obtained at 0.1 AM.When added to these combinations of stimulants, metiamide and atropine retained their respective specificities against stimulation by histamine and carbamylcholine, in that responses were inhibited to the level that was seen when the component of the pair that was not inhibited was given alone.The observation that histamine plus gastrin andThis work was presented, in part, at the meeting of the

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“…Perfusion system. We previously described in detail the perfusion system used in our studies of the totally isolated canine stomach (12)(13)(14)(15)(16). The description of this system will not be repeated here.…”

Section: Methodsmentioning

confidence: 99%

Effect of Prostaglandin-E2 on Gastric Secretion and on Gastric Circulation of Totally Isolated ex vivo Canine Stomach

Kowalewski¹,

Kolodej²

1974

Pharmacology

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Totally isolated canine stomachs, perfused with homologous blood, were used for the study of the effect of prostaglandin-E₂ (PGE₂) on gastric secretion and circulation. Ex vivo stomachs were stimulated by a constant infusion of histamine into the gastric artery. When histamine-induced secretion was stabilized, PGE₂ was introduced intra-arterially for 1 h. Doses of PGE₂tested were 500, 250 and 125 µg/h. After 1 h PGE₂ infusion was interrupted, but histamine stimulation continued. PGE₂ significantly inhibited the output of gastric juice, HCl and pepsin and reduced the concentration of HCl. The effect of PGE₂ was still very marked after it was discontinued. PGE₂ had a potent vasodilator action on gastric circulation: it reduced the gastric peripheral resistance and the gastric arterial perfusion pressure. This hemodynamic action of PGE₂ was present only during infusion of the inhibitor.

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Potentiation between Pentagastrin and Histamine in Stimulation of Secretion from Totally Isolated <i>ex vivo</i> Canine Stomach

Cited by 6 publications

References 9 publications

Correlation between the Outputs of Pepsin and HCl by Isolated, <i>ex vivo</i> Perfused, Canine Stomach Stimulated with Humoral and Cholinergic Drugs

Correlation between the Outputs of Pepsin and HCl by Isolated, <i>ex vivo</i> Perfused, Canine Stomach Stimulated with Humoral and Cholinergic Drugs

The Interaction of Histamine with Gastrin and Carbamylcholine on Oxygen Uptake by Isolated Mammalian Parietal Cells

Effect of Prostaglandin-E<sub>2</sub> on Gastric Secretion and on Gastric Circulation of Totally Isolated <i>ex vivo</i> Canine Stomach

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