Pouch colon associated with anorectal malformations fails to show spontaneous contractions but responds to acetylcholine and histamine in vitro

Tyagi, Preeti; Mandal, Maloy B; Mandal, S; Patne, Shashikant C. U.; Gangopadhyay, A. N.

doi:10.1016/j.jpedsurg.2009.04.032

Cited by 17 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Reduction in these contractile proteins may lead to reduced contractility of the pouch and pouch dysfunction. In 1 study on 12 patients, the muscle strips of the pouch lacked normal spontaneous contractions but retained acetylcholine and histamine-induced contractility [12] . The authors proposed that the CPC pouch is an abnormally functional and developed tissue and should undergo resection.…”

Section: Discussionmentioning

confidence: 99%

“…There have been a few studies in relation to the histopathology of CPC: four on the pouch [9][10][11][12] and 1 on the colovesical fistula [13] . Histopathology and IHC were performed on all 5 types of CPC.…”

Section: Discussionmentioning

confidence: 99%

“…This additional muscle layer, along with the disrupted muscularis propria, may cause additional functional obstruction, thereby explaining the poor postoperative results observed in types 1 and 2 CPC. The presence of an additional muscle coat has been previously described in cases of functional intestinal obstruction, intestinal atresia, and some types of CPC [11,12,[16][17][18] . In 1 study, it was seen in 5 patients with type 1/ type 2 CPC [11] ; in another study, it was seen in only 1 patient [12] .…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Histopathological and Immunohistochemical Findings in Congenital Pouch Colon: A Prospective Study

Udawat¹,

Nunia²,

Mathur³

et al. 2017

Pathobiology

View full text Add to dashboard Cite

Background: There is a paucity of literature on the histopathological aspects of congenital pouch colon (CPC) and immunohistochemical (IHC) assessment has not been reported. So we planned to study the histopathological and IHC findings within the spectrum of CPC and compare the findings with the normal colon. Methods: This is a descriptive prospective study on CPC patients. There were 49 cases of CPC (42 males and 7 females) and 13 controls. Histological examination was done using hematoxylin and eosin and Masson trichrome stain. IHC analysis was done with actin, myosin, and desmin antibodies, and neuron-specific enolase and S100 markers for counting ganglionic cells. Results: Histologically, congestion, edema and hemorrhage were seen in mucosa, submucosa, and serosa. Muscle layers were disrupted and divided into bands. An additional muscle coat inside of the muscularis propria was seen in CPC types 1 and 2. Mature ganglionic cells were reduced and muscle layers showed reduced and patchy positivity for smooth muscle actin, myosin, and desmin compared to a normal colon. Conclusions: Histopathological and IHC findings suggest that CPC has distinct defects in the neuromusculature.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Histopathological and Immunohistochemical Findings in Congenital Pouch Colon: A Prospective Study

Udawat¹,

Nunia²,

Mathur³

et al. 2017

Pathobiology

View full text Add to dashboard Cite

show abstract

“…[14] Till date, there are only a few studies that demonstrate the contractile mechanisms of human gut musculature of pediatric age group. Earlier observations reported that the contractile responses in anorectal malformations with pouch colon which failed to demonstrate spontaneous contractions [16] whereas another observation demonstrated spontaneous contractions in 66% of Hirschsprung's disease patients. [17] However, these studies failed to draw a comparative conclusion and relations between Hirschsprung's disease and anorectal malformations.…”

Section: Introductionmentioning

confidence: 95%

“…[16,17] In brief, the muscle strip was placed in Dale's organ bath (10 ml) containing Krebs Ringer at 28°C and continuously bubbled with 100% O 2 . The strip was placed under an initial tension of 0.5 g and then allowed to equilibrate for 30 min.…”

Section: Recording Of Contractile Responsesmentioning

confidence: 99%

A comparative study of in vitro contractility between gut tissues of Hirschsprung's disease and other gut malformations

Tiwari¹,

Singh²,

Pandey³

et al. 2017

Natl J Physiol Pharm Pharmacol

View full text Add to dashboard Cite

Background: Hirschsprung's disease and other gut malformations commonly present with obstructive features of gut in pediatric age group. Problem of obstruction persists even after resection and anastomosis operation. Aims and Objectives: This in vitro comparative study was performed to assess the gut contractility to chemical mediators such as Acetylcholine and Histamine between Hirschsprung's disease and other gut malformations. Materials and Methods: The longitudinal muscle strips of Hirschsprung's cases and other gut malformations (non-Hirschsprung's cases) were placed in Dales organ bath containing Krebs-Ringer solution, continuously bobbled with 100% O2 at 28°C. Gut contractions were recorded using Power Lab 4/ST system and was analyzed using software CHART-5 for windows. Control contractions were recorded against initial tension of 0.5 g. Subsequently, agonist (acetylcholine, histamine)-induced contractions were recorded before and after appropriate antagonists (atropine, pheniramine). Before values of agonist-induced contractions of Hirschsprung and non-Hirschsprung's cases were compared. Values of agonistinduced contractions obtained after pretreatment with antagonists were also compared between Hirschsprung and nonHirschsprung's cases. Results: Acetylcholine enhanced contractions in non-Hirschsprung's cases and it caused small increase in amplitude of contractions in Hirschsprung's cases. Atropine pretreatment blocked acetylcholine-induced contractions significantly in non-Hirschsprung's cases, whereas it failed to block in the Hirschsprung's cases. Histamine augmented contractions in both Hirschsprung and non-Hirschsprung's cases. H1 antagonist, pheniramine failed to block the contractility in both the cases. Conclusion: Findings of this study suggested that acetylcholine increased gut contractility significantly in non-Hirschsprung's cases involving muscarinic-cholinergic pathways, whereas histamine increased gut contractility in both Hirschsprung's disease and non-Hirschsprung's cases and it is not mediated by H1 receptors.

show abstract

Congenital Pouch Colon

Saxena¹,

Mathur²

2017

Pediatric Surgery

View full text Add to dashboard Cite

Pouch colon associated with anorectal malformations fails to show spontaneous contractions but responds to acetylcholine and histamine in vitro

Cited by 17 publications

References 20 publications

Histopathological and Immunohistochemical Findings in Congenital Pouch Colon: A Prospective Study

Histopathological and Immunohistochemical Findings in Congenital Pouch Colon: A Prospective Study

A comparative study of in vitro contractility between gut tissues of Hirschsprung's disease and other gut malformations

Congenital Pouch Colon

Contact Info

Product

Resources

About