PP159. In women with previous pregnancy hypertension, cardiovascular risk biomarkers may be modulated by haptoglobin genotype

Bicho, Manuel; Matos, Andreia; Silva, A.P.; Macedo, Camilla Bento de; Afonso, Conceição; Areias, Maria José; Rebelo, Irene

doi:10.1016/j.preghy.2012.04.270

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In respect to DEGs, Hsd11b2 gene was overexpressed in infected groups compared to controls. The Hsd11b2 gene is a marker for pregnancy hypertension (Bicho et al, 2012;Majchrzak-Celinska et al, 2017). When the Hsd11b2 gene is overexpressed, it can cause placental dysfunction, especially in early-onset preeclampsia.…”

Section: Discussionmentioning

confidence: 99%

A Transcriptome Analysis: Various Reasons of Adverse Pregnancy Outcomes Caused by Acute Toxoplasma gondii Infection

et al. 2020

View full text Add to dashboard Cite

Background: Toxoplasma gondii (T. gondii) is an obligate intracellular parasite, which can affect the pregnancy outcomes in infected females by damaging the uterus, and the intrauterine environment as well as and the hypothalamus resulting in hormonal imbalance. However, the molecular mechanisms underlying the parasite-induced poor pregnancy outcomes and the key genes regulating these mechanisms remain unclear. Therefore, this study aimed to analyze the gene expression in the mouse's uterus following experimentally-induced acute infection with T. gondii RH strain. Three groups of female mice were intraperitoneally injected with tachyzoites as follow; 3 days before pregnancy (FBD6), after pregnancy (FAD6), and after implantation (FID8) as the experimental groups. Another corresponding three groups served as control, were injected with normal saline at the same time. Transcriptome analysis of the total RNA extracted from both infected and non-infected mouse uterus samples was performed using RNA sequencing (RNA-Seq).Results: The three experimental groups (FBD6, FAD6, and FID8) had a total of 4,561, 2,345, and 2,997 differentially expressed genes (DEGs) compared to the controls. The significantly upregulated and downregulated DEGs were 2,571 and 1,990 genes in FBD6, 1,042 and 1,303 genes in FAD6 and 1,162 and 1,835 genes in FID8 group, respectively. The analysis of GO annotation, and KEGG pathway showed that DEGs were mainly involved in anatomical structure development, transport, cell differentiation, embryo development, hormone biosynthetic process, signal transduction, immune system process, phagosome, pathways in cancer, and cytokine-cytokine receptor interaction pathways. Frontiers in Physiology | www.frontiersin.org February 2020 | Volume 11 | Article 115 Zhou et al. Adverse Pregnancy and T. gondii InfectionConclusion: T. gondii infection can induce global transcriptomic changes in the uterus that may cause pregnancy hypertension, destruct the intrauterine environment, and hinder the normal development of placenta and embryo. Our results may help to understand the molecular mechanisms of the acute T. gondii infection, which could promote the development of new therapeutics or prophylactics for toxoplasmosis in pregnancy.Abbreviations: CD6A, mice injected with normal saline (NS) 3 days after pregnancy; CD6B, mice injected with NS 3 days before pregnancy; CD8, mice injected with NS in the fifth day of pregnancy; DEGs, differentially expressed genes; FAD6, mice infected with T. gondii 3 days after pregnancy; FBD6, mice infected with T. gondii 3 days before pregnancy; FID8, mice infected with T. gondii on the fifth day of pregnancy.

show abstract

Section: Discussionmentioning

confidence: 99%