PPARα, PPARγ and SREBP-1 pathways mediated waterborne iron (Fe)-induced reduction in hepatic lipid deposition of javelin goby Synechogobius hasta

Chen, Guanghui; Luo, Zhi; Chen, Feng; Shi, Xi; Song, Yong; Liu, Xu

doi:10.1016/j.cbpc.2017.04.003

Cited by 23 publications

(12 citation statements)

References 51 publications

(51 reference statements)

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“…Thus, the present study indicated that PPAR α , PPAR γ and SREBP-1 mediated the regulation of lipid deposition and metabolism in the fore- and mid-intestine in yellow catfish. Similarly, several studies reported that these signalling pathways widely mediated mineral element-induced changes in hepatic lipid deposition in fish ( 36 , 38 ) .…”

Section: Discussionmentioning

confidence: 88%

“…Zinc content. For the determination of Zn content, the fore-and mid-intestinal samples were digested in 3 ml concentrated nitric acid at 110°C for 72 h, and diluted to appropriate concentrations for Zn content using ICP-MS (36) . Quality assurance/quality control procedures included analysis of three method blanks (purified water), two certified biological reference tissues (DORM-2 and DORM-4; National Research Council of Canada) and two randomly selected duplicate samples per twenty samples.…”

Section: Samples Analysismentioning

confidence: 99%

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Dietary zinc addition influenced zinc and lipid deposition in the fore- and mid-intestine of juvenile yellow catfishPelteobagrus fulvidraco

et al. 2017

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The present study explored the mechanisms of dietary Zn influencing Zn and lipid deposition in the fore- and mid- intestine in yellow catfish Pelteobagrus fulvidraco, and investigated whether the mechanism was intestinal-region dependent. For this purpose, yellow catfish were fed three diets containing Zn levels of 8·83, 19·20 and 146·65 mg Zn/kg, respectively. Growth performance, intestinal TAG and Zn contents as well as activities and mRNA expression of enzymes and genes involved in Zn transport and lipid metabolism in the fore- and mid-intestine were analysed. Dietary Zn increased Zn accumulation as well as activities of Cu-, Zn-superoxide dismutase and ATPase in the fore- and mid-intestine. In the fore-intestine, dietary Zn up-regulated mRNA levels of ZnT1, ZnT5, ZnT7, metallothionein (MT) and metal response element-binding transcription factor-1 (MTF-1), but down-regulated mRNA levels of ZIP4 and ZIP5. In the mid-intestine, dietary Zn up-regulated mRNA levels of ZnT1, ZnT5, ZnT7, MT and MTF-1, but down-regulated mRNA levels of ZIP4 and ZIP5. Dietary Zn reduced TAG content, down-regulated activities of 6-phosphogluconate dehydrogenase (6PGD), glucose-6-phosphate dehydrogenase (G6PD), malic enzyme (ME) and fatty acid synthase (FAS) activities, and reduced mRNA levels of 6PGD, G6PD, FAS, PPARγ and sterol-regulator element-binding protein (SREBP-1), but up-regulated mRNA levels of carnitine palmitoyltransferase IA, hormone-sensitive lipase (HSLa), adipose TAG lipase (ATGL) and PPARα in the fore-intestine. In the mid-intestine, dietary Zn reduced TAG content, activities of G6PD, ME, isocitrate dehydrogenase and FAS, down-regulated mRNA levels of 6PGD, G6PD, FAS, acetyl-CoA carboxylase a, PPARγ and SREBP-1, but up-regulated mRNA expression of HSLa, ATGL and PPARγ. The reduction in TAG content following Zn addition was attributable to reduced lipogenesis and increased lipolysis, and similar regulatory mechanisms were observed between the fore- and mid-intestine.

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Section: Discussionmentioning

confidence: 88%

Section: Samples Analysismentioning

confidence: 99%

Dietary zinc addition influenced zinc and lipid deposition in the fore- and mid-intestine of juvenile yellow catfishPelteobagrus fulvidraco

et al. 2017

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“…PPARγ and SREBP1 are two transcription factors that activate numerous downstream genes in the adipogenesis signalling pathway for lipid synthesis and storage (Chen et al, 2017; Lefterova & Lazar, 2009). Here, only intestinal ppar γ abundance was suppressed in response to starvation, but srebp1 transcription remained unaffected (Figure 4f, g).…”

Section: Discussionmentioning

confidence: 99%

Effect of starvation on intestinal morphology, digestive enzyme activity and expression of lipid metabolism‐related genes in javelin goby ( Synechogobius hasta )

Zhou¹,

Qu³

et al. 2021

Aquaculture Research

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To assess the effects of starvation on Synechogobius hasta intestinal function and morphology, fish were starved for 3, 7 and 14 days, followed by the measurement of histological, physiological and transcriptional responses in the intestine. Intestinal length was significantly lower in S. hasta starved for 7 and 14 days (p < 0.05). Intestinal amylase activity was significantly decreased upon fasting (p < 0.05), whereas a lower level of intestinal lipase activity was not observed until the end of the trial. After 7-day starvation, the intestine of starved S. hasta exhibited exfoliated villus cells, increased vacuoles and widened cell interstices. Regarding the expression of lipid metabolismassociated genes, poststarvation transcript levels of lipoprotein lipase, carnitine palmitoyltransferase 1 a and fatty acid transport protein 1 significantly increased with longer food deprivation periods (p < 0.05). Meanwhile, downregulation of stearoyl-CoA desaturase 1 was observed in the intestine upon starvation (p < 0.05). In summary, prolonged starvation (>14 days) not only disrupted the intestinal structure, which may further weaken digestive potential, but also attenuated intestinal lipid deposition by diminishing lipid biosynthesis, as well as upregulating lipolysis and fatty acid transport. Therefore, our findings provide preliminary data for further characterization of metabolic traits in starved S. hasta, which may have important implications for determining the appropriate starvation time applied in fasting/re-feeding regimes, as well as the sustainable development of S. hasta and other fish.

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“…Five treatments were designed as follows: the control, 20μg/mL nano-ZnO (nano-ZnO group), 2 μM TPEN (TPEN group), 20 μg/mL nano-ZnO + 2 μM TPEN (nano-ZnO + TPEN group), 2 μM Dynasore (Dynasore group), and 20 μg/mL nano-ZnO + 2 μM Dynasore (nano-ZnO + Dynasore group). The concentrations of Zn and the corresponding inhibitors were selected according to our and other studies [ 39 , 40 ]. Each treatment had triplicate groups.…”

Section: Methodsmentioning

confidence: 99%

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Chen

et al. 2021

IJMS

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Nano-sized zinc oxide (nano-ZnO) affects lipid deposition, but its absorption patterns and mechanisms affecting lipid metabolism are still unclear. This study was undertaken to investigate the molecular mechanism of nano-ZnO absorption and its effects on lipid metabolism in the intestinal tissues of a widely distributed freshwater teleost yellow catfish Pelteobagrus fulvidraco. We found that 100 mg/kg dietary nano-ZnO (H-Zn group) significantly increased intestinal Zn contents. The zip6 and zip10 mRNA expression levels were higher in the H-Zn group than those in the control (0 mg/kg nano-ZnO), and zip4 mRNA abundances were higher in the control than those in the L-Zn (50 mg/kg nano-ZnO) and H-Zn groups. Eps15, dynamin1, dynamin2, caveolin1, and caveolin2 mRNA expression levels tended to reduce with dietary nano-ZnO addition. Dietary nano-ZnO increased triglyceride (TG) content and the activities of the lipogenic enzymes glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and isocitrate dehydrogenase (ICDH), upregulated the mRNA abundances of lipogenic genes 6pgd, fatty acid synthase (fas), and sterol regulatory element binding protein 1 (srebp1), and reduced the mRNA expression of farnesoid X receptor (fxr) and small heterodimer partner (shp). The SHP protein level in the H-Zn group was lower than that in the control and the L-Zn group markedly. Our in vitro study indicated that the intestinal epithelial cells (IECs) absorbed nano-ZnO via endocytosis, and nano-Zn-induced TG deposition and lipogenesis were partially attributable to the endocytosis of nano-ZnO in IECs. Mechanistically, nano-ZnO-induced TG deposition was closely related to the metal responsive transcription factor 1 (MTF-1)-SHP pathway. Thus, for the first time, we found that the lipogenesis effects of nano-ZnO probably depended on the key gene shp, which is potentially regulated by MTF1 and/or FXR. This novel signaling pathway of MTF-1 through SHP may be relevant to explain the toxic effects and lipotoxicity ascribed to dietary nano-ZnO addition.

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PPARα, PPARγ and SREBP-1 pathways mediated waterborne iron (Fe)-induced reduction in hepatic lipid deposition of javelin goby Synechogobius hasta

Cited by 23 publications

References 51 publications

Dietary zinc addition influenced zinc and lipid deposition in the fore- and mid-intestine of juvenile yellow catfishPelteobagrus fulvidraco

Dietary zinc addition influenced zinc and lipid deposition in the fore- and mid-intestine of juvenile yellow catfishPelteobagrus fulvidraco

Effect of starvation on intestinal morphology, digestive enzyme activity and expression of lipid metabolism‐related genes in javelin goby ( Synechogobius hasta )

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

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