PpIAA1 and PpERF4 form a positive feedback loop to regulate peach fruit ripening by integrating auxin and ethylene signals

Wang, Xiaobei; Pan, Lei; Wang, Yan; Meng, Junren; Deng, Li; Niu, Liang; Liu, Hui; Ding, Yifeng; Yao, Jia‐Long; Nieuwenhuizen, Nicolaas Jacobus; Ampomah-Dwamena, Charles; Lü, Zhenhua; Cui, Guochao; Wang, Zhiqiang; Zeng, Wenfang

doi:10.1016/j.plantsci.2021.111084

Cited by 45 publications

(31 citation statements)

References 52 publications

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“…PpIAA1 in peach can bind to the PpACS1 promoter to activate its expression. The overexpression of PpIAA1 in tomato accelerates fruit ripening and shortens fruit shelf life by increasing the production of ETH and controlling the expression of ripening-related genes [ 97 ]. On the other hand, the overexpression of the IAA-responsive gene PpSAUR43 down-regulates the cell wall degradation gene PpPG and delays the softening of peach fruit [ 98 ].…”

Section: Phytohormones Regulation Of Fruit Softeningmentioning

confidence: 99%

“…PpERF4 in peach activates the transcription of PpACO1 and PpIAA1 genes by binding to their promoters. PpIAA1 interacts with PpPRF4 to form a complex, which activates the transcription of ABA biosynthesis genes ( PpNCED2/3 ) and fruit-softening genes ( PpPG1 ), and ultimately regulates peach fruit ripening and softening by integrating IAA and ETH signals [ 97 ]. Recently, Cheng et al [ 122 ] found that PpERF/ABR1 directly binds to the PpPG promoter to activate its expression, which also induces peach fruit softening, and the overexpression of PpERF/ABR1 promotes the production of ETH in accelerating fruit softening.…”

Section: Transcriptional Regulation Of Fruit Softeningmentioning

confidence: 99%

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Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

Peng

Liu

et al. 2022

IJMS

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Fruit softening that occurs during fruit ripening and postharvest storage determines the fruit quality, shelf life and commercial value and makes fruits more attractive for seed dispersal. In addition, over-softening results in fruit eventual decay, render fruit susceptible to invasion by opportunistic pathogens. Many studies have been conducted to reveal how fruit softens and how to control softening. However, softening is a complex and delicate life process, including physiological, biochemical and metabolic changes, which are closely related to each other and are affected by environmental conditions such as temperature, humidity and light. In this review, the current knowledge regarding fruit softening mechanisms is summarized from cell wall metabolism (cell wall structure changes and cell-wall-degrading enzymes), plant hormones (ETH, ABA, IAA and BR et al.), transcription factors (MADS-Box, AP2/ERF, NAC, MYB and BZR) and epigenetics (DNA methylation, histone demethylation and histone acetylation) and a diagram of the regulatory relationship between these factors is provided. It will provide reference for the cultivation of anti-softening fruits.

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Section: Phytohormones Regulation Of Fruit Softeningmentioning

confidence: 99%

Section: Transcriptional Regulation Of Fruit Softeningmentioning

confidence: 99%

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

Peng

Liu

et al. 2022

IJMS

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“…Furthermore, ERFs are the key transcription factors responsible for ethylene downstream signal transduction. Previous study in peach fruit reported that PpERF4 enhances the transcription of PpACO1 by binding to its promoter in ( Wang et al, 2021 ). ERF4 also represses the expression of ACS1 and ACO1 by interacting with JAZ in apple fruit ( Hu et al, 2022 ).…”

Section: Discussionmentioning

confidence: 98%

1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes

et al. 2022

Front. Plant Sci.

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Ethylene burst is an important sign of the initiation of postharvest mango ripening and softening is a typical characteristic of fruit ripening. However, the intrinsic link between ethylene release and fruit softening during ripening of postharvest mangoes is still not clear. The aim of this study was to investigate the effects of ethylene and its action inhibitor 1-methylcyclopropene (1-MCP) on fruit softening and ripening and the underlying regulatory mechanisms. Results showed that ethephon (ETH) promoted ethylene release and enhanced MDA content and activities of cell wall degrading enzymes, whereas 1-MCP treatment exhibited an opposite effect. Moreover, real-time quantitative polymerase chain reaction indicated that the transcription levels of genes involved in cell wall degradation (MiPG, Miβ-GAL and MiPE), ethylene biosynthesis (MiACO1 and MiACS6) and ethylene response factor (MiERF8) were remarkably induced by ETH. Correlation analysis further revealed that the production of ethylene was significantly negatively correlated with firmness, but positively correlated with MDA content, activities of cell wall degrading enzymes and expressions of MiPG and Miβ-GAL. Furthermore, yeast one hybrid (Y1H) assay showed that MiERF2 and MiERF8 could directly bind to the promotor of MiPG and then regulate its transcription. These findings suggest that ethylene production is closely associated with fruit softening, and MiERF2 and MiERF8 and MiPG may play crucial roles in regulation of ripening and softening of postharvest mangoes.

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“…To identify TCP genes in peach, sequences of Arabidopsis TCP genes were downloaded from PlantTFDB (v4.0) [ 50 ] and used as queries in BLAST searches against the peach genome. Twenty PpTCP genes were identified in peach and named PpTCP1 through PpTCP20 based on their chromosome positions.…”

Section: Methodsmentioning

confidence: 99%

PpTCP18 is upregulated by lncRNA5 and controls branch number in peach (Prunus persica) through positive feedback regulation of strigolactone biosynthesis

Wang

Yan

et al. 2022

Horticulture Research

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Branch number is an important agronomic trait in peach (Prunus persica) trees because plant architecture affects fruit yield and quality. Although breeders can select varieties with different tree architecture, the biological mechanisms underlying architecture remain largely unclear. In this study, a pillar peach (‘Zhaoshouhong’) and a standard peach (‘Okubo’) were compared. ‘Zhaoshouhong’ was found to have significantly fewer secondary branches than ‘Okubo’. Treatment with the synthetic strigolactone (SL) GR24 decreased branch number. Transcriptome analysis indicated that PpTCP18 (a homologous gene of the Arabidopsis thaliana BRC1) expression was negatively correlated with strigolactone synthesis gene expression, indicating that PpTCP18 may play an important role in peach branching. Yeast one-hybrid, electrophoretic mobility shift, dual-luciferase assays and PpTCP18-knockdown in peach leaf buds indicated that PpTCP18 could increase expression of PpLBO1, PpMAX1, and PpMAX4. Furthermore, transgenic Arabidopsis plants overexpressing PpTCP18 clearly exhibited a reduced primary rosette-leaf branches. Moreover, lncRNA sequencing and transient expression analysis revealed that lncRNA5 targeted PpTCP18, significantly increasing PpTCP18 expression. These results provide insights into the mRNA and lncRNA network in the peach SL signaling pathway and indicate that PpTCP18, a transcription factor downstream of SL signaling, was involved in positive feedback regulation of SL biosynthesis. This role of PpTCP18 may represent a novel mechanism in peach branching regulation. Our study improves current understanding of the mechanisms underlying peach branching and provides theoretical support for genetic improvement of peach tree architecture.

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PpIAA1 and PpERF4 form a positive feedback loop to regulate peach fruit ripening by integrating auxin and ethylene signals

Cited by 45 publications

References 52 publications

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes

PpTCP18 is upregulated by lncRNA5 and controls branch number in peach (Prunus persica) through positive feedback regulation of strigolactone biosynthesis

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