Practical Aspects of Hyaluronan Based Medical Products

Kuo, Jing Wen

doi:10.1201/9781420037647

Cited by 46 publications

(40 citation statements)

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“…Although the composition of HA appears to be consistent across animal species, there may be differences in the molecular weight of this polymer across anatomical regions within an individual and between species (14,15). High-molecular-weight HA occurs in the loose connective tissue and has the role of maintaining cell integrity and water content.…”

Section: Interstitial Matrix Compositionmentioning

confidence: 99%

Factors Influencing the Use and Interpretation of Animal Models in the Development of Parenteral Drug Delivery Systems

Martinez

2011

AAPS J

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Abstract. Depending upon the drug and drug delivery platform, species-specific physiological differences can lead to errors in the interspecies extrapolation of drug performance. This manuscript provides an overview of the species-specific physiological variables that can influence the performance of parenteral dosage forms such as in situ forming delivery systems, nanoparticles, microspheres, liposomes, targeted delivery systems, lipophilic solutions, and aqueous suspensions. Also discussed are those factors that can influence the partitioning of therapeutic compounds into tumors, the central nervous system and the lymphatics. Understanding interspecies differences in the movement and absorption of molecules is important to the interpretation of data generated through the use of animal models when studying parenteral drug delivery.

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Section: Interstitial Matrix Compositionmentioning

confidence: 99%

Factors Influencing the Use and Interpretation of Animal Models in the Development of Parenteral Drug Delivery Systems

Martinez

2011

AAPS J

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“…11,12 Moreover, medical products containing HyA have been used for several decades confirming its biocompatibility, familiarity, and ease of adoption for clinical applications. [13][14][15] The benefits of combining collagen and HyA into a biomaterial include enhanced effects on extracellular matrix protein production and material tunability. 16,17 With these advantages in mind, we have recently reported on the self-assembly between collagen and HyA into membrane structures.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic Potential of BMP-2-Releasing Self-Assembled Membranes

Chung

Chien

Aguado

et al. 2013

Tissue Engineering Part A

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We report here the use of novel self-assembling collagen-hyaluronic acid (HyA) membranes to deliver bone morphogenetic protein-2 (BMP-2) for orthopedic applications. Prior work has demonstrated that collagen-HyA membranes are formed initially through electrostatic interactions between the oppositely charged collagen and HyA molecules, and that membrane growth is driven by osmotic pressure imbalances between the collagen and HyA solutions. The purpose of this study was to investigate the potential of incorporating charged growth factors such as BMP-2 within the membrane for regenerative medicine applications. Membrane material properties, protein mass loss, and release kinetics of BMP-2, as well as biocompatibility and osteogenic potential in vitro and in vivo using a subcutaneous mouse model were assessed. Scanning electron microscopy and mechanical testing confirmed no loss of structural or mechanical integrity upon BMP-2 incorporation into the membranes. Slow and steady release of the growth factor was demonstrated with 17% of total loaded BMP-2 released over the course of 49 days. To test biocompatibility and osteogenic potential in vitro, human mesenchymal stem cells were cultured on collagen-HyA membranes and showed greater proliferation rates (for up to 28 days) on membranes without BMP-2, but a greater alkaline phosphatase activity and osteocalcin production on membranes releasing BMP-2. In vivo subcutaneous implantation of the membranes showed a minimal immune response with osteoblasts and mineral deposits present in the ectopic site for BMP-2-releasing membranes, further demonstrating the potential of the BMP-2-releasing membranes to induce osteogenic differentiation. This study presents a novel strategy to create self-assembled membranes using two biocompatible molecules that can deliver bioactive agents in a sustained manner to induce a local regenerative response.

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“…It also promotes wound healing, and has been used commercially in artificial tears preparations for over 30 years (Kuo, 2005) with an established safety record. It is a high-molecular weight polysaccharide (107 kDa) consisting of a backbone of alternating groups of (1-4) glucuronic acid and (1-3)-N-acetyl glucosamine (Sadhasivam, et al 2013).…”

Section: Discussionmentioning

confidence: 99%