Practical DNA markers to Estimate Apple (MalusÃ‚Â x domestica Borkh.) Skin Color, Ethylene Production and Pathogen Resistance

Kikuchi, Teppei; Kasajima, Ichiro; Morita, Mizuki; Yoshikawa, Nobuyuki

doi:10.4172/2376-0354.1000211

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Such disadvantage of dCAPS primers derives from the structure of these primers, and seems to have been inevitable until recently. In our recent report, however, the difference in DNA fragments was successfully elongated to nearly 100 bp [15]. This was done by simply tor.…”

Section: Adaptor Attachment To Dcaps and Degenerate Primersmentioning

confidence: 99%

“…Promoters isolated from horticultural plants by adaptor-PCR method also contribute to transgenic modification of ornamental traits [9,11]. Even better adaptor-related techniques such as DS4 adaptor and adaptor-fused DNA markers were devised recently [10,15,16]. Together with the advances in the protocols for preparation of pure genomic DNA, DNA adaptors are providing much more applications to genetic studies.…”

Section: High-quality Dna Extraction and Purification For Successful Pcr Analysesmentioning

confidence: 99%

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DNA Adaptor Technology as PCR Application

Kasajima¹

2018

Current Trends Anal Bioanal Chem

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PCR (polymerase chain reaction) is a popular analytical method in genetics of organisms. By using gene-specific primers, a small single region in the huge genomic DNA is selectively amplified by PCR. Thus, PCR is quite frequently used for determination of gene alleles and to identify nucleotide sequences of genes. For these variety of analytical purposes, PCR method is often combined with the other DNA-related methods such as sequence-dependent DNA digestion with restriction enzymes, bisulfite reaction of genomic DNA for estimation of cytosine methylation, and usage of adaptor DNA for advanced analyses. This review will focus on the properties of adaptor DNAs, and will introduce how these short synthetic DNAs help analysis of DNA sequences.

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Section: Adaptor Attachment To Dcaps and Degenerate Primersmentioning

confidence: 99%

Section: High-quality Dna Extraction and Purification For Successful Pcr Analysesmentioning

confidence: 99%

DNA Adaptor Technology as PCR Application

Kasajima¹

2018

Current Trends Anal Bioanal Chem

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“…Skin color of apple fruit is regulated by the MYB1 gene, which is a transcription factor functioning for anthocyanin synthesis (Takos et al 2006). Apple cultivars with wild-type MYB1 bear red fruits, whereas apple cultivars with mutated MYB1 bear yellow or orange fruits (Kikuchi et al 2017). Skin colors measured by PCM of 21 apple cultivars are plotted on color circle in Figure 2B, for demonstration of the effect of MYB1 mutation on apple skin color.…”

Section: Genetic Regulation Of Plant Colorsmentioning

confidence: 99%

Measuring plant colors

Kasajima

2019

Plant Biotechnology

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Plant colors such as 'green leaf ' and 'red apple' are often described based on human sense, even in scientific papers. On the other hand, colors are measured based on colorimetric principles in some papers, especially in the studies of horticultural plants. The science of color measurements ('colorimetry') is not included in any of the popular lectures in schools and universities, thus the principles of color measurements would not be understood by most researchers. The present review will overview the principles of colorimetry, and will introduce colorimetric methods which can be used for scientific measurement of plant colors. That is to say, the reflection spectrum of visible light (380-780 nm) is measured at 5-nm intervals on the surface of leaves or petals in 'Spectrometric Color Measurement' (SCM). The spectral data is multiplied with RGB or XYZ color matching functions and integrated to obtain RGB or XYZ intensities. Alternatively, approximate RGB values are directly obtained in 'Photographic Color Measurement' (PCM). RGB/XYZ intensities are further calculated to obtain 'hue' , 'saturation' , and 'lightness' , the three factors of colors. Colorimetric insights into genetic regulations (such as MYB gene) and physiological regulations (such as alexandrite effect) of plant colors are also described.

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