Practical tips and new trends in electrochemical biosensing of cancer-related extracellular vesicles

Sfragano, Patrick Severin; Pillozzi, Serena; Condorelli, Gerolama; Palchetti, Ilaria

doi:10.1007/s00216-023-04530-z

Cited by 6 publications

(2 citation statements)

References 121 publications

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“…Due to the advantages of high selectivity, high sensitivity, simple equipment, and low price, electrochemical sensors have been widely used for the detection of extracellular tumor markers 4 , 36 – 38 . The following sections summarize recent advances in the detection of CTCs, ctDNA, and exosomes in body fluids using electrochemical sensors.…”

Section: Electrochemical Sensingmentioning

confidence: 99%

Current research status of tumor cell biomarker detection

Jiang,

Lin,

Chen

et al. 2023

Microsyst Nanoeng

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With the annual increases in the morbidity and mortality rates of tumors, the use of biomarkers for early diagnosis and real-time monitoring of tumor cells is of great importance. Biomarkers used for tumor cell detection in body fluids include circulating tumor cells, nucleic acids, protein markers, and extracellular vesicles. Among them, circulating tumor cells, circulating tumor DNA, and exosomes have high potential for the prediction, diagnosis, and prognosis of tumor diseases due to the large amount of valuable information on tumor characteristics and evolution; in addition, in situ monitoring of telomerase and miRNA in living cells has been the topic of extensive research to understand tumor development in real time. Various techniques, such as enzyme-linked immunosorbent assays, immunoblotting, and mass spectrometry, have been widely used for the detection of these markers. Among them, the detection of tumor cell markers in body fluids based on electrochemical biosensors and fluorescence signal analysis is highly preferred because of its high sensitivity, rapid detection and portable operation. Herein, we summarize recent research progress in the detection of tumor cell biomarkers in body fluids using electrochemical and fluorescence biosensors, outline the current research status of in situ fluorescence monitoring and the analysis of tumor markers in living cells, and discuss the technical challenges for their practical clinical application to provide a reference for the development of new tumor marker detection methods.

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Section: Electrochemical Sensingmentioning

confidence: 99%

Current research status of tumor cell biomarker detection

Jiang,

Lin,

Chen

et al. 2023

Microsyst Nanoeng

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“…Electrochemical biosensors are currently mainly used for detecting tumor markers, tumor-related genes, and the tumor microenvironment. To more comprehensively assess the status and treatment effect of tumors, more types of electrochemical biosensors can be developed, such as detecting extracellular vesicles of tumor cells, tumor stem cells, tumor immune cells etc [18].…”

Section: Expanding Detection Range and Typesmentioning

confidence: 99%

Recent Advances in the Application of Electrochemical Biosensors in Tumor Therapy

Zhang

2023

IJBLS

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The progress in the application of electrochemical biosensors in tumor therapy aims to explore and study the latest developments and future trends of electrochemical biosensors in tumor therapy. This article first introduces the concept and classification of electrochemical biosensors, elaborates on the preparation methods and detection techniques of various electrochemical biosensors, and then reviews the application of electrochemical biosensors in tumor therapy. Finally, this article points out the research directions and challenges of electrochemical biosensors in improving sensitivity and specificity, expanding detection range and types, realizing multiplex detection and integration, and achieving portability and intelligence.

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