Practical use of microsatellite markers to manageVitis viniferagermplasm: Molecular identification of grapevine samples collected blindly in D.O. “El Bierzo” (Spain)

Identification and relationships of accessions grown in the grapevine (Vitis vinifera L.) Germplasm Bank of Castilla y Léon (Spain) and the varieties authorized in the VQPRD areas of the region by SSR-marker analysis

“…The DNA profiles of five accessions of the sample resulted unique after comparison of data with previous works (Sefc et al 2000;Ibáñez et al 2003;Martín et al 2003;Núñez et al 2004;Santiago et al 2005): 'Calagraña', 'Doradilla 2', 'Cenicienta', 'Juliana' and 'Pan y Carne 2'. These profiles have not been previously reported, and most of them belong to autochthonous varieties ('Calagraña', 'Cenicienta', 'Juliana', and 'Pan y Carne'), from which no previous written references or plant material in other grape collections have been found.…”

“…Therefore, a reliable genetic identification of all the accessions is needed to remove them from the bank. SSR (simple sequence repeats) or microsatellites have demonstrated to be a powerful tool for cultivar identification, and have been extensively used for characterizations of grapevine collections (Lopes et al 1999;Dangl et al 2001;Ibáñez et al 2003;Martín et al 2003;Núñez et al 2004;Ortiz et al 2004), and also for checking for genetic relationships and reconstructing pedigrees (Bowers and Meredith 1997;Sefc et al 1997Sefc et al , 1998Bowers et al 1999;Benjak et al 2005;Costantini et al 2005). In addition, SSR analysis allows a direct comparison of the results obtained by different groups, and the use of common databases (Lopes et al 1999;).…”

Vitis vinifera genotyping toolbox to highlight diversity and germplasm identification

“…Among the advantages of SSR is the use of species-specific primers, and the presence of increased variability and reproducibility ( Núñez et al., 2004 ). Data from SSR analyses may be stored in publicly available databases.…”

The use of AFLP and morphological markers to study Iranian grapevine germplasm to avoid genetic erosion

“…The availability of molecular tools to screen biodiversity among plant genomes (Karp et al, 1998) can resolve these problems. Some of the most commonly used molecular tools have already proved effective in grapevine (Labra et al, 2001a; and, in the last 10 years, microsatellite DNA analysis has been used successfully for grapevine genotyping (Bowers et al, 1996;1999;Labra et al, 2003;Núñez et al, 2004;Merdinoglu et al, 2005) and for pedigree analysis (Bowers and Meredith, 1997;Vouillamoz et al, 2003). *Author for correspondence.…”