Pre-Dna Synthesis Haemoperfusion

Ryan, C. J.; Courtney, J. M.; Gibbons, Mark J.; Aslam, Mohammed; Batten, John; Fleming, Jeanette; Wood, C. B.; Paul, Jeff

doi:10.1007/978-1-349-07283-5_23

Cited by 4 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The basis of our approach to artificial liver support has been the utilization of extracorporeal circuits in the rat in order to study the relationship between liver support systems, hepatic coma, detoxification, and liver regeneration (8,9,(11)(12)(13)(18)(19)(20)(21)(22). The suitability of the membrane plasma separation stage for the evaluation of single and/or multiple sorbents (multisorbent regimen) in normal animals and those with experimentally induced acute FHF was reported in detail elsewhere (8,9,12,22).…”

Section: Discussionmentioning

confidence: 99%

Multisorbent Plasma Perfusion in Fulminant Hepatic Failure: Effects of Duration and Frequency of Treatment in Rats with Grade III Hepatic Coma

Ryan¹,

Anilkumar²,

Ben-Hamida³

et al. 2001

Artificial Organs

View full text Add to dashboard Cite

Using the model of galactosamine-induced fulminant hepatic failure in the rat, the effects of multisorbent plasma perfusion over Asahi uncoated spherical charcoal, Plasorba (BR-350) resin, and an endotoxin removing adsorbent (polymyxin B-sepharose) were determined in Grade III hepatic coma animals by studying survival as influenced by timing, duration, and frequency of treatment. The effects of treatment on liver cell proliferation and endotoxin removal also were examined. The results demonstrate that duration and frequency of treatment are major contributing factors in the successful application of nonbiological membrane-based multisorbent liver support systems. Examination of the regenerative activity in the liver indicates an enhanced proliferative response following multisorbent plasma perfusion compared with untreated fulminant hepatic failure (FHF) paired controls. Utilizing an endotoxin removal adsorbent alone, a marked reduction in systemic levels of endotoxin in FHF was demonstrated compared with nonperfused FHF paired controls. Despite current emphasis on bioartificial liver support systems, plasma purification by multisorbent systems offers a simple method for the removal of circulating toxic metabolites in general together with specific toxin removal.

show abstract

Section: Discussionmentioning

confidence: 99%

Multisorbent Plasma Perfusion in Fulminant Hepatic Failure: Effects of Duration and Frequency of Treatment in Rats with Grade III Hepatic Coma

Ryan¹,

Anilkumar²,

Ben-Hamida³

et al. 2001

Artificial Organs

View full text Add to dashboard Cite

show abstract

Artificial Liver Support Systems: Design of Preclinical Evaluation Programmes

Ryan¹,

Aslam²,

Cohen³

et al. 1988

The Influence of New Technology on Medical Practice

View full text Add to dashboard Cite

Repeated Membrane Plasma Separation with On‐Line Sorbent Treatment of Plasma in the Conscious Rat

et al. 1986

View full text Add to dashboard Cite

Repeated membrane plasma separation with on-line sorbent treatment of plasma was performed in unrestrained and conscious rats, thus avoiding the possible effects of repeated stress and exposure to anesthetic agents. The procedure was well tolerated, even after four consecutive perfusions. Blood flow was 0.7 ml/min, with a transmembrane pressure of 18.6 mm Hg and plasma filtration rate of 0.21 ml/min. This allowed 12 ml of plasma (greater than 1 plasma volume) to be treated within 1 h. Levels of albumin, immunoglobulin G, and C3 remained constant during each perfusion and were not significantly different from those of animals subjected to control procedures. Results obtained from filtrate were comparable at 15 and 60 min with values obtained from whole blood, with sieving coefficients of approximately 1. Leukocyte, erythrocyte, and thrombocyte counts remained unchanged during each plasma perfusion. A progressive rise in leukocyte counts occurred following successive perfusions, but this was true also of animals subjected to control procedures.

show abstract

Pre-Dna Synthesis Haemoperfusion

Cited by 4 publications

References 12 publications

Multisorbent Plasma Perfusion in Fulminant Hepatic Failure: Effects of Duration and Frequency of Treatment in Rats with Grade III Hepatic Coma

Multisorbent Plasma Perfusion in Fulminant Hepatic Failure: Effects of Duration and Frequency of Treatment in Rats with Grade III Hepatic Coma

Artificial Liver Support Systems: Design of Preclinical Evaluation Programmes

Repeated Membrane Plasma Separation with On‐Line Sorbent Treatment of Plasma in the Conscious Rat

Contact Info

Product

Resources

About