Pre-exposure to Staphylococcal enterotoxin A exacerbates the pulmonary allergic eosinophil recruitment in rats

Airway exposure to staphylococcal enterotoxin A potentiates allergen-induced bone marrow eosinophilia and trafficking to peripheral blood and airways

“…Interestingly, SEA alone (with no OVA challenge) significantly increased the eosinophil influx into the lung section, as observed at 4 to 48 h preexposure. This is consistent with previous studies showing the presence of pulmonary inflammatory infiltrates in animals exposed to SEA alone (17,29,30). It is intriguing, however, that SEA exposure alone promotes eosinophil infiltration into the lung with no accompanying changes in BM and circulating blood.…”

“…Mice were actively sensitized with a subcutaneous injection (0.4 ml) of 100 g of OVA (grade V) mixed with 1.6 mg Al(OH) 3 in 0.9% NaCl (day 0). One week later (day 7), mice received a second subcutaneous injection of 100 g OVA (0.4 ml) (29). At days 14 and 15, sensitized mice were intranasally challenged with OVA (10 g/50 l) twice a day, thus resulting in four challenges (at day 14, the first challenge occurred at time zero and the second challenge 6 h later; at day 15, the third challenge occurred at time zero and the fourth challenge 6 h later).…”

“…Repeated intranasal application of SEB in mice causes a lymphocyte-dependent bronchial inflammation associated with airway hyperresponsiveness (17). Intranasal administration of SEA (29) and SEB (16,18) also exacerbates the pulmonary eosinophilic inflammation in ovalbumin (OVA)-induced allergic airway disease. Interestingly, the pulmonary cell infiltration after instillation of SEA and SEB is accompanied by a late increase in bone marrow (BM) granulocyte number (7,8,29).…”

Розвиток Імунної Відповіді При Стафілококовій Пневмонії (Частина 1)

“…Взаимодействие SE с эпителиальными клетками слизистой оболочки способствует их активации и продукции хемокинов, рекрутиру-ющих нейтрофилы и макрофаги [21]. Альвеоляр-ные макрофаги под влиянием SE усиливают про-дукцию IL-8/СХСL8, рекрутирующего нейтро-филы; эотаксина, рекрутирующего эозинофилы; и IL-6 [14,39].…”

Enhancement of the pulmonary allergic granulocyte recruitment in rats exposed to DMTI-II, a Kunitz-type inhibitor isolated from Dimorphandra mollis seeds

“…Fourteen days later, sensitized (and non-sensitized) rats were intranasally challenged with 1 mg of OVA (0.2 ml) or instilled with phosphate-buffered saline (PBS; 0.2 ml). Bronchoalveolar lavage (BAL) fluid, bone marrow, and circulating blood were obtained at selected times thereafter, and processed according to our previous experience [28,29].…”