Pre-hatching exposure to N2B27 medium improves post-hatching development of bovine embryos in vitro

Ramos‐Ibeas, Priscila; Pérez-Gómez, Alba; González-Brusi, Leopoldo; Quiroga, Alejandra C.; Bermejo-Álvarez, Pablo

doi:10.1016/j.theriogenology.2023.04.018

Cited by 8 publications

(2 citation statements)

References 37 publications

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“…Bovine trophoblast stem cell lines are also available, and these cells have the capacity to proliferate as mononucleated TE or differentiate into binucleated TE [168]. Lastly, recent strides have been made in exploring the origins of EPI-PE differentiation using extended cultures with media formulations used in primed embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture systems [169][170][171]. These media formulations permit blastocysts to undergo complete HYPO migration while promoting EPI cell survival, albeit at a slower rate than what occurs in vivo.…”

Section: Elongationmentioning

confidence: 99%

Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle

Speckhart

Oliver

Ealy

2023

Animals

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Several key developmental events are associated with early embryonic pregnancy losses in beef and dairy cows. These developmental problems are observed at a greater frequency in pregnancies generated from in-vitro-produced bovine embryos. This review describes critical problems that arise during oocyte maturation, fertilization, early embryonic development, compaction and blastulation, embryonic cell lineage specification, elongation, gastrulation, and placentation. Additionally, discussed are potential remediation strategies, but unfortunately, corrective actions are not available for several of the problems being discussed. Further research is needed to produce bovine embryos that have a greater likelihood of surviving to term.

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Section: Elongationmentioning

confidence: 99%

Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle

Speckhart

Oliver

Ealy

2023

Animals

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“…For Experiment 2 (effect of AA during post-hatching culture), presumptive zygotes were cultured in 25 μL drops of SOF medium supplemented with 5% fetal calf serum under mineral oil up to Day 7. Beyond Day 7, embryo culture was performed as described in Ramos-Ibeas et al (2020) but starting exposure to N2B27 medium at an earlier stage (Day 7), a modification that improves post-hatching developmental rates (Ramos-Ibeas et al 2023). N2B27 medium is composed of 1:1 Neurobasal and DMEM/F12 media supplemented with penicillin/streptomycin, 2 mM glutamine, N2 and B27 supplements (Gibco).…”

Section: In Vitro Production Of Bovine Blastocystsmentioning

confidence: 99%

Effect of arachidonic acid on pre- and post-hatching in vitro bovine embryo development

González-Brusi

Pérez-Gómez

Quiroga

et al. 2023

Reprod. Fertil. Dev.

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Context Arachidonic acid (AA) is the precursor of prostaglandins, which may play autocrine roles during early embryo development. Aims To test the developmental effects of addition of AA to pre- and post-hatching culture media on in vitro–produced bovine embryos. Methods Pre-hatching effects of AA were tested by culturing bovine zygotes in synthetic oviductal fluid (SOF) supplemented with 100 or 333 μM AA. Post-hatching effects of AA were tested by culturing Day 7 blastocysts in N2B27 supplemented with 5, 10, 20 or 100 μM AA up to Day 12. Key results Pre-hatching development to blastocyst was completely abrogated at 333 μM AA, whereas blastocyst rates and cell numbers were not altered at 100 μM AA. Impaired post-hatching development was observed at 100 μM AA, whereas no effect on survival rates was noted at 5, 10 and 20 μM AA. However, a significant reduction in Day 12 embryo size was observed at 10 and 20 μM AA. Hypoblast migration, epiblast survival and formation of embryonic-disc-like structures were unaffected at 5–10 μM AA. AA exposure downregulated the genes PTGIS, PPARG, LDHA and SCD in Day 12 embryos. Conclusions Pre-hatching embryos are mostly irresponsive to AA, whereas AA was observed to have negative effects during early post-hatching development. Implications AA does not improve in vitro bovine embryo development and is not required up to early post-hatching stages.

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Early Cell Lineage Formation in Mammals: Complexity, Species Diversity, and Susceptibility to Disruptions Impacting Embryo Viability

Latham

2024

Molecular Reproduction Devel

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The emergence of the earliest cell lineages in mammalian embryos is a complex process that utilizes an extensive network of chromatin regulators, transcription factors, cell polarity regulators, and cellular signaling pathways. These factors and pathways operate over a protracted period of time as embryos cleave, undergo compaction, and form blastocysts. The first cell fate specification event separates the pluripotent inner cell mass from the trophectoderm lineage. The second event separates pluripotent epiblast from hypoblast. This review summarizes over 50 years of study of these early lineage forming events, addressing the complexity of the network of interacting molecules, cellular functions and pathways that drive them, interspecies differences, and aspects of these mechanisms that likely underlie their high susceptibility to disruption by numerous environmental factors that can compromise embryo viability, such as maternal health and diet, environmental toxins, and other stressors.

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Pre-hatching exposure to N2B27 medium improves post-hatching development of bovine embryos in vitro

Cited by 8 publications

References 37 publications

Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle

Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle

Effect of arachidonic acid on pre- and post-hatching in vitro bovine embryo development

Early Cell Lineage Formation in Mammals: Complexity, Species Diversity, and Susceptibility to Disruptions Impacting Embryo Viability

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