Pre‐implantation diagnosis

Monk, Marilyn

doi:10.1002/bies.950080603

Cited by 10 publications

(1 citation statement)

References 24 publications

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“…An alternative involves sex selection of preimplantation embryos. This would allow the transfer of female embryos to the mother's uterus after in vitro fertilization (IVF), avoiding the need for distressing therapeutic abortion of wanted pregnancies (Penketh and McLaren, 1987;Whittingham and Penketh, 1987;McLaren, 1987;Monk, 1988).…”

Section: Introductionmentioning

confidence: 99%

Rapid sexing of human embryos by non‐radioactive in situ hybridization: Potential for preimplantation diagnosis of X‐linked disorders

Penketh¹,

Delhanty²,

Berghe³

et al. 1989

Prenatal Diagnosis

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Sixty spare human embryos at various stages of preimplantation development were prepared for cytogenetic analysis. Fluorescent staining of those with metaphases allowed scoring for the presence of a Y chromosome. In situ hybridization was then performed using a biotinylated Y-specific sequence, and the probe was detected by a standard streptavidin-linked alkaline phosphatase system. This enabled comparison of the chromosomal sex with that obtained after in situ hybridization in 28 embryos, and the sexing result obtained by the two methods was concordant in all cases. A further 21 embryos in which no metaphase chromosomes were obtained were sexed by biotinylated in situ hybridization only. Overall, 66 per cent of male interphase nuclei demonstrated a Y-specific hybridization signal. Results were obtained in under 24 h, which may permit the sexing of an embryo biopsied during cleavage and the transfer of sexed embryos at the blastocyst stage to the mother's uterus in the same cycle as oocytes are collected for in vitro fertilization.

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Section: Introductionmentioning

confidence: 99%

Rapid sexing of human embryos by non‐radioactive in situ hybridization: Potential for preimplantation diagnosis of X‐linked disorders

Penketh¹,

Delhanty²,

Berghe³

et al. 1989

Prenatal Diagnosis

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Preimplantation sexing and diagnosis of hypoxanthine phosphoribosyl transferase deficiency in mice by biochemical microassay

et al. 1990

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Hypoxanthine phosphoribosyl transferase (HPRT)-deficient male embryos derived from heterozygous (carrier) female mice were diagnosed by biochemical microassay of X-chromosome-coded HPRT activity in a single cell taken from the 8-cell embryo or in 5-10 cells sampled from the blastocyst. In the latter procedure, carrier female blastocysts could also be distinguished from affected males, and normal males and females, as having intermediate HPRT activity in the sampled trophectoderm cells. During the assay procedures, the operated preimplantation embryos were cultured. They were then transferred, in batches as diagnosed, to recipient females. The resulting fetuses were sexed by gonad morphology and assayed for HPRT activity. All those identified as HPRT-negative embryos by biopsy at the 8-cell or blastocyst stages were indeed HPRT-negative males. The heterozygous females were also correctly identified by the trophectoderm biopsy procedure. The sex of an embryo can also be diagnosed by HPRT activity dosage in a single blastomere taken from 8-cell embryos from a normal mating and cultured for 12 hours before assay. Both X chromosomes are active in female morulae and the blastomeres sampled from female preimplantation embryos have twice the X-coded HPRT activity compared to those from the male embryos. The accuracy of this procedure for sexing was again verified by transfer of the putative male and putative female embryos into recipient females.

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Sexing the Pre-embryo

West¹

1991

The Embryo

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Pre‐implantation diagnosis

Cited by 10 publications

References 24 publications

Rapid sexing of human embryos by non‐radioactive in situ hybridization: Potential for preimplantation diagnosis of X‐linked disorders

Rapid sexing of human embryos by non‐radioactive in situ hybridization: Potential for preimplantation diagnosis of X‐linked disorders

Preimplantation sexing and diagnosis of hypoxanthine phosphoribosyl transferase deficiency in mice by biochemical microassay

Sexing the Pre-embryo

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