Pre infusion, post thaw CD34+ peripheral blood stem cell enumeration as a predictor of haematopoietic engraftment in autologous haematopoietic cell transplantation

D’Rozario, James; Parisotto, Robin; Stapleton, Jennifer; Gidley, Alison; Owen, Denise

doi:10.1016/j.transci.2014.02.021

Cited by 23 publications

(22 citation statements)

References 29 publications

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“…Similar concern had been reported recently regarding the poor correlation of potency results in cord blood stem cells obtained from attached segments with those from the corresponding cord blood units . This stands in contrast to many authors who assume that post‐thaw flow cytometric analyses reflect the composition of cells infused and can be used to draw conclusions on the outcome in terms of time to engraftment . This seems to be justified in some, but not in all cases.…”

Section: Discussionmentioning

confidence: 50%

“…The use of post‐thaw flow cytometric potency testing is divisive but performed in many centres. While it is well accepted that the composition of the cryopreservation solution plays an important role in post‐thaw cell recovery , the influence of temperature during the 30 min staining period on cell survival is not generally known and reported post‐thaw recovery results are rather controversial . We were able to show highly significant differences in viable cell recovery when thawed cells were stained at 4°C vs. RT.…”

Section: Discussionmentioning

confidence: 68%

“…D′Rozario et al . report a 33% loss of viable CD34 + cells and Reich‐Slotky et al . an 86% recovery, but only 57%–74% viability .…”

Section: Introductionmentioning

confidence: 96%

“…Colony‐forming unit (CFU) assays are used as well as flow cytometric analyses to determine the CD34 cell number infused into patients. In addition, various freezing media and different dimethyl‐sulphoxide (DMSO) concentrations, with or without the addition of starch, are described . Clarke et al ., Stylianou et al .…”

Section: Introductionmentioning

confidence: 99%

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Relevance of flow cytometric enumeration of post‐thaw leucocytes: influence of temperature during cell staining on viable cell recovery

et al. 2016

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 68%

“…D′Rozario et al . report a 33% loss of viable CD34 + cells and Reich‐Slotky et al . an 86% recovery, but only 57%–74% viability .…”

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Relevance of flow cytometric enumeration of post‐thaw leucocytes: influence of temperature during cell staining on viable cell recovery

et al. 2016

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“…Regarding engraftment, we found that higher postthaw CD34+ cell counts were associated with faster engraftment of ANCs, PLTs, and RBCs. PLT engraftment was the most sensitive parameter, as already described by others . For completeness of cellular engraftment data, we also assessed RBC engraftment, and found that it depended on CD34+ count infused.…”

Section: Discussionmentioning

confidence: 99%

The value of the post‐thaw CD34+ count with and without DMSO removal in the setting of autologous stem cell transplantation

et al. 2018

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BACKGROUND CD34+ cell count correlates with engraftment potency after autologous stem cell transplantation. Assessment of CD34+ mainly occurs after apheresis and before cryopreservation with dimethyl sulfoxide (DMSO). The influence of postthaw CD34+ cell numbers over time to engraftment is not well studied, and determination of postthaw CD34+ cell counts is challenging for a variety of reasons. The aim of this retrospective study was to systematically assess the value of postthaw CD34+ cell counts in autologous grafts with and without DMSO removal. STUDY DESIGN AND METHODS Between January 2008 and December 2015, 236 adult patients underwent a total of 292 autologous stem cell transplantations. Median age at transplantation was 56 years, and the main indication was multiple myeloma (60%). DMSO removal was done in 96 grafts (33%), either by centrifugation or by Sepax method. RESULTS Patients receiving grafts containing DMSO showed a significantly faster platelet (p = 0.02) and RBC (p = 0.001) engraftment. DMSO removal was not associated with fewer infusion‐related adverse events. We observed a good correlation between CD34+ cell count after apheresis and CD34+ cell count after thawing/washing (r = 0.931). Ninety grafts (31%) showed a significant loss of viable CD34+ cells, which translated into a delayed engraftment. CONCLUSION DMSO removal was associated with delayed platelet and RBC engraftment without preventing adverse events. CD34+ cell enumeration after thawing remains difficult to perform, but grafts showing higher cell loss during cryopreservation and thawing are associated with slower engraftment. Prospective studies on the role of DMSO removal and postthaw CD34+ enumeration using defined protocols are needed.

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A critical assessment of dose effects of post‐thaw CD34 on autologous stem cell transplantation treatment of haematological malignancies

Duarte¹,

Butler

Atkinson³

et al. 2023

eJHaem

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A consensus threshold of pre‐cryopreservation CD34‐positive cells (CD34s) has been used as the minimum dose to initiate autologous stem cell transplantation (ASCT). Advances in cryopreservation posed a debate whether post‐thaw CD34s might be a superior surrogate instead. We addressed the debate in this retrospective study of 217 adult ASCTs in five different haematological malignancies treated at a single centre. We showed that post‐thaw CD34s was highly correlated with pre‐cryopreservation CD34s (r = 0.97) and explained ∼2.2% (p = 0.003) of the variation of the post‐thaw total nucleated cell viability that however had no power to predict engraftment outcomes. After stratifying the ASCT cases into four dose groups based on post‐thaw CD34s reinfused, stepwise multivariate regression analyses detected significant effects in dose group and interactions with diseases for neutrophil and platelet recovery respectively. The significant dose effects and interactions were triggered by two technical outliers in the low dose group, and disappeared in the repeated regressions after exclusion of the outliers where disease and age were the significant predictors remained. Our data clearly support the validity of the consensus threshold in ASCT applications but also highlight neglected conditions where monitoring post‐thaw CD34s and clinical attributes are valuable.

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Pre infusion, post thaw CD34+ peripheral blood stem cell enumeration as a predictor of haematopoietic engraftment in autologous haematopoietic cell transplantation

Cited by 23 publications

References 29 publications

Relevance of flow cytometric enumeration of post‐thaw leucocytes: influence of temperature during cell staining on viable cell recovery

Relevance of flow cytometric enumeration of post‐thaw leucocytes: influence of temperature during cell staining on viable cell recovery

The value of the post‐thaw CD34+ count with and without DMSO removal in the setting of autologous stem cell transplantation

A critical assessment of dose effects of post‐thaw CD34 on autologous stem cell transplantation treatment of haematological malignancies

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