Preanalysis Storage Conditions Influence the Measurement of Brain-Derived Neurotrophic Factor Levels in Peripheral Blood

Tsuchimine, Shoko; Sugawara, Norio; Ishioka, Masamichi; Yasui‐Furukori, Norio

doi:10.1159/000358061

Cited by 49 publications

(52 citation statements)

References 17 publications

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“…Plasma BDNF levels change within a day and can be influenced by environmental factors and correlate positively with platelets activation state [46,47,48] and might well reflect the physio-pathological condition of the body [49]. However, plasma BDNF is quite unstable [50] and sensitive to preparation procedure [51,52,53]. …”

Section: Discussionmentioning

confidence: 99%

“…Concerning methodological aspects, it is well-known that different anticoagulants, temperatures, and delays in sample centrifugation, as well as conditions and time of sample storage influence the measurements of BDNF [46,51,53,61]. Similarly, the ELISA kits used to measure BDNF have different performance in terms of detection range, sensitivity, and inter-assay variations [62].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Association between Obesity and Circulating Brain-Derived Neurotrophic Factor (BDNF) Levels: Systematic Review of Literature and Meta-Analysis

Sandrini

Minno

Amadio

et al. 2018

IJMS

108

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Reduction in brain-derived neurotrophic factor (BDNF) expression in the brain as well as mutations in BDNF gene and/or of its receptor are associated to obesity in both human and animal models. However, the association between circulating levels of BDNF and obesity is still not defined. To answer this question, we performed a meta-analysis carrying out a systematic search in electronic databases. Ten studies (307 obese patients and 236 controls) were included in the analysis. Our data show that obese patients have levels of BDNF similar to those of controls (SMD: 0.01, 95% CI: −0.28, 0.30, p = 0.94). The lack of difference was further confirmed both in studies in which BDNF levels were assessed in serum (MD: −0.93 ng/mL, 95% CI: −3.34, 1.48, p = 0.45) and in plasma (MD: 0.15 ng/mL, 95% CI: −0.09, 0.39, p = 0.23). Data evaluation has shown that some bias might affect BDNF measurements (e.g., subject recruitment, procedures of sampling, handling, and storage), leading to a difficult interpretation of the results. Standardization of the procedures is still needed to reach strong, affordable, and reliable conclusions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Association between Obesity and Circulating Brain-Derived Neurotrophic Factor (BDNF) Levels: Systematic Review of Literature and Meta-Analysis

Sandrini

Minno

Amadio

et al. 2018

IJMS

108

View full text Add to dashboard Cite

show abstract

“…In plasma (EDTA, He, citrate) BDNF concentrations increased over 48 h of storage at 4 or 25 ℃, while in serum they were stable (184). BDNF has a short half-life in plasma of rats (0.92 min) (185).…”

Section: Pre-analytical Factorsmentioning

confidence: 98%

Measuring myokines with cardiovascular functions: pre-analytical variables affecting the analytical output

Lombardi¹,

Sansoni²,

Banfi³

2017

Ann. Transl. Med.

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Abstract:In the last few years, a growing number of molecules have been associated to an endocrine function of the skeletal muscle. Circulating myokine levels, in turn, have been associated with several pathophysiological conditions including the cardiovascular ones. However, data from different studies are often not completely comparable or even discordant. This would be due, at least in part, to the whole set of situations related to the preparation of the patient prior to blood sampling, blood sampling procedure, processing and/or store. This entire process constitutes the pre-analytical phase. The importance of the preanalytical phase is often not considered. However, in routine diagnostics, the 70% of the errors are in this phase. Moreover, errors during the pre-analytical phase are carried over in the analytical phase and affects the final output. In research, for example, when samples are collected over a long time and by different laboratories, a standardized procedure for sample collecting and the correct procedure for sample storage are acknowledged. In this review, we discuss the pre-analytical variables potentially affecting the measurement of myokines with cardiovascular functions.

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“…Nevertheless, studies investigating BDNF lack reproducibility [3]. Confounders, which are often not taken into account, include the pre-analytical treatment of samples [4] and reproducibility of the analytical method [5]. …”

Section: Introductionmentioning

confidence: 99%

“…High positive correlations between serum and plasma BDNF concentrations in healthy volunteers have been reported [7]. However, other studies could not reproduce these findings [4,8]. Physical exercise, which was found to increase BDNF concentrations, influenced BDNF concentrations in serum to a higher degree than in plasma [9].…”

Section: Introductionmentioning

confidence: 99%

Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations

Polyakova

Schlögl

Sacher

et al. 2017

IJMS

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Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at −80 °C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to rs = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations.

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Preanalysis Storage Conditions Influence the Measurement of Brain-Derived Neurotrophic Factor Levels in Peripheral Blood

Cited by 49 publications

References 17 publications

Association between Obesity and Circulating Brain-Derived Neurotrophic Factor (BDNF) Levels: Systematic Review of Literature and Meta-Analysis

Association between Obesity and Circulating Brain-Derived Neurotrophic Factor (BDNF) Levels: Systematic Review of Literature and Meta-Analysis

Measuring myokines with cardiovascular functions: pre-analytical variables affecting the analytical output

Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations

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