Precise in situ localization of NCAM, ETS1, and D11S29 on human meiotic chromosomes

Bello, M. Josefa; Salagnon, N.; Rey, Juan A.; Guichaoua, Marie-Roberte; Bergé-Lefranc, Jean-Louis; Jordan, Bertrand; Luciani, J. M.

doi:10.1159/000132828

Cited by 14 publications

(3 citation statements)

References 11 publications

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“…This gene localization is distinct from that for CD6 on chromosome 11 (40). Chromosome 11 also carries the neural cell adhesion molecule locus at 11q23.1 encoding the neural cell adhesion molecule (41). Of the genes closely related to ALCAM, the gene for the melanoma-associated glycoprotein MUC18 (42) has not been mapped, but another member of the Ig superfamily of adhesion molecules, B-CAM, which encodes the human F8/G253 antigen, is located on chromosome 19q13.2q-13.3 (43).…”

Section: Cos-cd6mentioning

confidence: 85%

Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.

Bowen

Patel

et al. 1995

The Journal of Experimental Medicine

355

280

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SummaryAntibody-blocking studies have demonstrated the role of CD6 in thymocyte-thymic epithelial (TE) cell adhesion. Here we report that CD6 expressed by COS cells mediates adhesion to TE cells and that this interaction is specifically blocked with an anti-CD6 monodonal antibody (mAb) or with a mAb 04-81) that recognized a TE cell antigen. We isolated and expressed a cDNA clone encoding this antigen and show that COS cells transfected with this cDNA bind a CD6 immunoglobulin fusion protein (CD6-Rg). This antigen, which we named ALCAM (activated leukocyte-cell adhesion molecule) because of its expression on activated leukocytes, appears to be the human homologue of the chicken neural adhesion molecule BEN/SC-1/DM-GRASP. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. We prepared an ALCAM-Rg fusion protein and showed that it binds to COS cell transfectants expressing CD6, demonstrating that AI.CAM is a CD6 ligand. The observations that ALCAM is also expressed by activated leukocytes and that both ALCAM and CD6 are expressed in the brain suggest that ALCAM-CD6 interactions may play a role in the binding of T and B cells to activated leukocytes, as well as in interactions between cells of the nervous system.

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Section: Cos-cd6mentioning

confidence: 85%

Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.

Bowen

Patel

et al. 1995

The Journal of Experimental Medicine

355

280

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“…. 1986Bello et al, 1989). As shown by the present study, meiotic chromosomes can also be used in combination with FISH to obtain a more precise sublo calization of the signal, and this precision may even be further augmented by using less-condensed female pachytene chromo somes (Jhanwar et al.…”

Section: Discussionmentioning

confidence: 65%

Assignment of the human gene for pregnancy-associated plasma protein A (PAPPA) to 9q33.1 by fluorescence in situ hybridization to mitotic and meiotic chromosomes

Silahtaroglu¹,

Tümer

Kristensen³

et al. 1993

Cytogenet Genome Res

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Low levels of pregnancy-associated plasma protein A (PAPPA) during the first trimester has been suggested as a biochemical indicator of pregnancies with aneuploid fetuses. Furthermore, the complete absence of PAPPA in pregnancies associated with Cornelia de Lange syndrome (CL) has suggested a causal connection between PAPPA and the development of CL. We have assigned the locus for PAPPA to chromosome region 9q33.1 on mitotic and meiotic chromosomes by fluorescence in situ hybridization, using a 3.7-kb partial PAPPA cDNA probe.

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“…The two adjacent 5S rDNA loci can be more clearly resolved in pachytene chromosome preparations. The use of pachytene preparations in order to resolve DNA target sites for loci that are close together in a chromosomal subregion has previously been reported for human loci (Bello et al, 1989, Hultén & Armstrong, 1994. FISH provides an efficient method for identifying the number and physical location of repeated sequences.…”

Section: Discussionmentioning

confidence: 99%

Physical mapping of DNA repetitive sequences to mitotic and meiotic chromosomes of Brassica oleracea var. alboglabra by fluorescence in situ hybridization

et al. 1998

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As part of a programme to integrate the genetic and physical chromosome maps of Brassica oleracea we have carried out fluorescence in situ hybridization (FISH) to mitotic and meiotic chromosomes of B. oleracea var. alboglabra, using repeat sequence DNA probes. We have confirmed that there are three 18S-5.8S-25S rDNA sites in the haploid genome and accurately determined their locations for the first time; two occur subtelomerically on the short arms of the two satellited acrocentric chromosomes (chromosomes 4 and 7), whereas the third site is adjacent to the centromere on the short arm of a large submetacentric chromosome (chromosome 2). 5S rDNA sequences are located on the long arm of this same chromosome, with closely adjacent major and minor loci. A highly repeated sequence (pBcKB4) colocalizes with the pericentromeric heterochromatin on all chromosomes, but six chromosomes exhibit very strong signals whereas the remaining three show only weak signals. The construction of a partial karyotype for B. oleracea var. alboglabra, based on several major cytogenetical landmarks provided by FISH localization of repetitive DNA sequences, provides a valuable framework for the future physical mapping of nonrepetitive sequences.

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Precise in situ localization of NCAM, ETS1, and D11S29 on human meiotic chromosomes

Cited by 14 publications

References 11 publications

Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.

Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.

Assignment of the human gene for pregnancy-associated plasma protein A (PAPPA) to 9q33.1 by fluorescence in situ hybridization to mitotic and meiotic chromosomes

Physical mapping of DNA repetitive sequences to mitotic and meiotic chromosomes of Brassica oleracea var. alboglabra by fluorescence in situ hybridization

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