Precise simultaneous quantification of methadone and cocaine in rat serum and brain tissue samples following their successive i.p. administration

Nakhla, D. S.; Hussein, Lobna A.; Magdy, Nancy; Abdallah, Inas A.; Hassan, Hazem E.

doi:10.1016/j.jchromb.2017.01.048

Cited by 6 publications

(3 citation statements)

References 48 publications

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“…2017 Identification of five substituted phenethylamine derivatives 5-MAPDB, 5-AEDB, MDMA methylene homolog, 6-Br-MDMA, and 5-APB-NBOMe by LC-QTOF-MS, GC-MS and NMR [ 1282 ]; analysis of cutting agents in Australian seizures of cocaine and heroin over six years [ 1283 ]; HPLC with dual UV detection for the simultaneous quantification of methadone and cocaine [ 1284 ]; fast determination of codeine, orphenadrine, promethazine, scopolamine, tramadol, and paracetamol in pharmaceutical formulations by CE [ 1285 ]; fentanyl and U-47700 in Norco pills bearing a Watson imprint [ 1286 ]; identification and analytical characterization of U-47700-Et and 4-F-Pentedrone [ 1287 ]; SERS, Raman, and DFT analyses of fentanyl and carfentanil [ 1288 , 1289 ]; cold EI based fast GC-MS analysis of cocaine and heroin (focus on the optimization of flow programming) [ 1290 ]; analysis of cutting agents in cocaine and heroin drug seizures in Australian [ 1283 ], determination of LSD and 25h-NBOMe by Square Wave Voltammetry [ 1291 ]; SERS methods detect trace levels of Cocaine, Heroin, Methamphetamine, and THC [ 1292 ]; colorimetric biosensor to detect methamphetamine and cocaine in biological and environmental matrices [ 1293 ] electrochemical oxidation of morphine and codeine by the application of a novel glassy carbon electrode modified with a hydroxyapatite-Fe3O4 nanoparticles/multiwalled carbon nanotubes composite (HA-FeNPs-MWCNTs/GCE) [ 1294 ]; stability of morphine and methadone in syringes analyzed by HPLC [ 1295 ]; synthetic agents off the darknet: a case of U-47700 and phenazepam abuse [ 1296 ]; method for determining synthetic sedative-hypnotics and sleep inducers, including barbital, benzodiazepam, zolpidem, and first-generation antihistamines, in adulterated products using Quadrupole-Orbitrap MS and UHPLC-MS/MS [ 1297 ];…”

Section: Routine and Improved Analyses Of Abused Substancesmentioning

confidence: 99%

Interpol review of controlled substances 2016–2019

Jones

Comparin

2020

Forensic Science International: Synergy

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Section: Routine and Improved Analyses Of Abused Substancesmentioning

confidence: 99%

Interpol review of controlled substances 2016–2019

Jones

Comparin

2020

Forensic Science International: Synergy

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“…Most tissue distribution studies are performed in animals models [ 16 , 17 ], while poor information is available about distribution in humans [ 18 , 19 , 20 ]. In addition, the currently available observations in humans are mainly focused on toxicological/forensic qualitative or semi-quantitative applications, serving more than a quantitative purpose in the context of pain management.…”

Section: Introductionmentioning

confidence: 99%

A Novel UHPLC-MS/MS Method for the Quantification of Seven Opioids in Different Human Tissues

et al. 2023

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Background: Opioids are considered the cornerstone of pain management: they show good efficacy as a first-line therapy for moderate to severe cancer pain. Since pharmacokinetic/pharmacodynamic information about the tissue-specific effect and toxicity of opioids is still scarce, their quantification in post-mortem autoptic specimens could give interesting insights. Methods: We describe an ultra-high-performance liquid chromatography coupled with tandem mass spectrometry method for the simultaneous quantification of methadone, morphine, oxycodone, hydrocodone, oxymorphone, hydromorphone and fentanyl in several tissues: liver, brain, kidney, abdominal adipose tissue, lung and blood plasma. The presented method has been applied on 28 autoptic samples from different organs obtained from four deceased PLWH who used opioids for palliative care during terminal disease. Results: Sample preparation was based on tissue weighing, disruption, sonication with drug extraction medium and a protein precipitation protocol. The extracts were then dried, reconstituted and injected onto the LX50 QSight 220 (Perkin Elmer, Milan, Italy) system. Separation was obtained by a 7 min gradient run at 40 °C with a Kinetex Biphenyl 2.6 µm, 2.1 × 100 mm. Concerning the analyzed samples, higher opioids concentrations were observed in tissues than in plasma. Particularly, O-MOR and O-COD showed higher concentrations in kidney and liver than other tissues (>15–20 times greater) and blood plasma (>100 times greater). Conclusions: Results in terms of linearity, accuracy, precision, recovery and matrix effect fitted the recommendations of FDA and EMA guidelines, and the sensitivity was high enough to allow successful application on human autoptic specimens from an ethically approved clinical study, confirming its eligibility for post-mortem pharmacological/toxicological studies.

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“…MDN causes up to 32% of opioid analgesics poisoning. [3][4][5][6][7][8] Medical research has shown that the required administered methadone dosage should be based on an evaluation performed by a qualified clinician through a recognized clinical process. 9,10 To achieve optimum treatment, new analytical methods and time-saving sample preparation techniques for the determination of methadone in blood extract samples have been used.…”

Section: Introductionmentioning

confidence: 99%

Optimization of New Sample Preparation Technique for the Determination of Methadone and Codeine in Plasma Sample by GC-FID

Jamil

2020

J. Braz. Chem. Soc.

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A continuous sample drop flow microextraction (CSDF-ME) technique was modified for the pretreatment of plasma samples in the determination of methadone and codeine. The limit of detection and linear range for methadone were 15.0 µg L −1 and 0.1-10.0 mg L −1 , respectively. The detection limit and linear range for codeine were 25.0 µg L −1 and 0.2-15.0 mg L −1 , respectively. The extraction recoveries were 41.6-52.1%, and the enrichment factors ranged from 65 to 80 for codeine and methadone, respectively. Intra-and interday precisions of the technique were calculated for codeine and methadone concentrations of 0.4 mg L −1 and were 4.2-7.6% and 2.5-5.1%, respectively. Also, the accuracy (error%) of method was in the range −10 to +7.5%. The applicability of the CSDF-ME-gas chromatography-flame ionization detector (GC-FID) technique was illustrated by the determination of the mentioned drugs in human plasma. The attained relative recoveries of methadone and codeine in the 90.0-107.5% range demonstrated the excellent capability of the optimized technique for the microextraction of the mentioned drugs from plasma. The CSDF-ME-GC-FID is a rapid, repeatable and very simple technique that requires a low sample amount.

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Precise simultaneous quantification of methadone and cocaine in rat serum and brain tissue samples following their successive i.p. administration

Cited by 6 publications

References 48 publications

Interpol review of controlled substances 2016–2019

Interpol review of controlled substances 2016–2019

A Novel UHPLC-MS/MS Method for the Quantification of Seven Opioids in Different Human Tissues

Optimization of New Sample Preparation Technique for the Determination of Methadone and Codeine in Plasma Sample by GC-FID

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