Precision phenotyping across the life cycle to validate and decipher drought-adaptive QTLs of wild emmer wheat (Triticum turgidum ssp. dicoccoides) introduced into elite wheat varieties

Abstract

Drought events or the combination of drought and heat conditions are expected to become more frequent due to global warming, and wheat yields may fall below their long-term average. One way to increase climate-resilience of modern high-yielding varieties is by their genetic improvement with beneficial alleles from crop wild relatives. In the present study, the effect of two beneficial QTLs introgressed from wild emmer wheat and incorporated in the three wheat varieties BarNir, Zahir and Uzan was studied under well-watered conditions and under drought stress using non-destructive High-throughput Phenotyping (HTP) throughout the life cycle in a single pot-experiment. Plants were daily imaged with RGB top and side view cameras and watered automatically. Further, at two time points, the quantum yield of photosystem II was measured with a top view FluorCam. The QTL carrying near isogenic lines (NILs) were compared with their corresponding parents by t-test for all non-invasively obtained traits and for the manually determined agronomic and yield parameters. Data quality of phenotypic traits (repeatability) in the controlled HTP experiment was above 85% throughout the life cycle and at maturity. Drought stress had a strong effect on growth in all wheat genotypes causing biomass reduction from 2% up to 70% at early and late points in the drought period, respectively. At maturity, the drought caused 47–55% decreases in yield-related traits grain weight, straw weight and total biomass and reduced TKW by 10%, while water use efficiency (WUE) increased under drought by 29%. The yield-enhancing effect of the introgressed QTLs under drought conditions that were previously demonstrated under field/screenhouse conditions in Israel, could be mostly confirmed in a greenhouse pot experiment using HTP. Daily precision phenotyping enabled to decipher the mode of action of the QTLs in the different genetic backgrounds throughout the entire wheat life cycle. Daily phenotyping allowed a precise determination of the timing and size of the QTLs effect (s) and further yielded information about which image-derived traits are informative at which developmental stage of wheat during the entire life cycle. Maximum height and estimated biovolume were reached about a week after heading, so experiments that only aim at exploring these traits would not need a longer observation period. To obtain information on different onset and progress of senescence, the CVa curves represented best the ongoing senescence of plants. The QTL on 7A in the BarNir background was found to improve yield under drought by increased biomass growth, a higher photosynthetic performance, a higher WUE and a “stay green effect.”

Engaging Precision Phenotyping to Scrutinize Vegetative Drought Tolerance and Recovery in Chickpea Plant Genetic Resources

“…However, in our study, when plants recovered from drought, photosynthetic capacity had not been permanently damaged and ΦPSIIh returned to well-watered levels, similar to a field study with soybean (G. max) and drought stress [16]. Similarly, [47] ΦPSIIr was higher under drought stress.…”

“…The HTP system used in this study has already proven useful for studies to test the biomass development and color change of wheat Near Isogenic Lines throughout the life cycle in phases of varying drought stress and also the biomass development of barley PGR under drought stress in the vegetative stage [47,55]. The greenhouse conditions met the temperature requirements for chickpea and were combined with an irrigation system that simulates drought stress followed by recovery during vegetative development.…”

“…Color-related traits have been used previously to represent physiological responses to challenging environmental conditions in HTP in wheat (T. aestivum; T. durum), barley (H. vulgare), and maize (Z. mays), as well as rice (O. sativa) and lettuce (Lactuca sativa) [47,54,55,61,62].…”

“…Each pot (18.5 cm height × 14.9 cm diameter) was filled with Klasman substrate No. 2 described in [47]. After 14 days of pre-cultivation in a regular greenhouse chamber outside the HTP system at 24 • C during the day and 20 • C during the night, with a relative humidity of 67% during the day and 76% during the night, a daylight period of greenhouse lights of 15 h (from 6 am to 9 pm), and manual watering, the plants were transferred to the greenhouse with the HTP system with the same growing conditions.…”

“…HTP has the strength to assess the temporal dynamics of agriculturally important traits such as biomass or plant height and reveal traits related to plant physiology such as WUE and the efficiency of photosynthesis [50,54]. Furthermore, color-related information was used to study the progress of drought stress and senescence in barley (H. vulgare), rice (Oryza sativa), maize (Z. mays), and wheat (Triticum aestivum, T. durum) [47,[53][54][55].…”